Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy
The existence of more of 16,000 varieties of quinoa accessions around the world has caused a disregard on their structural and phytochemical characteristics. Most of such accessions belong to cultivars settled in Colombia. The goal of this research was to evaluate the structural attributes and antio...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/10/10/2159 |
| _version_ | 1797513375382503424 |
|---|---|
| author | Miguel García-Parra Diego Roa-Acosta Víctor García-Londoño Brigitte Moreno-Medina Jesús Bravo-Gomez |
| author_facet | Miguel García-Parra Diego Roa-Acosta Víctor García-Londoño Brigitte Moreno-Medina Jesús Bravo-Gomez |
| author_sort | Miguel García-Parra |
| collection | DOAJ |
| description | The existence of more of 16,000 varieties of quinoa accessions around the world has caused a disregard on their structural and phytochemical characteristics. Most of such accessions belong to cultivars settled in Colombia. The goal of this research was to evaluate the structural attributes and antioxidant capacities from six quinoa cultivars with high productive potential from central regions in Colombia. This study used middle-range infrared spectroscopy (IR-MIR) to determine the proteins, starch and lipids distinctive to quinoa grains. Ultra-high-performance liquid chromatography electrospray ionization Orbitrap, along with high-resolution mass spectrometry (UHPLC/ESI-Orbitrap MS), were also used to identify the existence of polyphenols in cultivars. The antioxidant capacity was determined through DPPH, ABTS and FRAP. The spectrums exhibited significant variances on the transmittance bands associated with 2922 cm<sup>−1</sup>, 1016 cm<sup>−1</sup> and 1633 cm<sup>−1</sup>. Moreover, the intensity variations on the peaks from the secondary protein structure were identified, mainly on the bands associated with β-Sheet-1 and -2, random coil α elice and β-turns-2 and -3. Changes found in the ratios 996 cm<sup>−1</sup>/1014 cm<sup>−1</sup> and 1041 cm<sup>−1</sup>/1014 cm<sup>−1</sup> were associated with the crystalline/amorphous affinity. Regarding the antioxidant capacity, great differences were identified (<i>p</i> < 0.001) mainly through FRAP methods, while the phenolic acids and flavonoids were determined by UHPLC/ESI-Orbitrap MS techniques. The presence of apigenin and pinocembrin on grains was reported for the first time. Titicaca and Nariño were the most phytochemically diverse quinoa seeds. |
| first_indexed | 2024-03-10T06:15:41Z |
| format | Article |
| id | doaj.art-d52c03e8f9fc4a6ba13510da1e2a0b12 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T06:15:41Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d52c03e8f9fc4a6ba13510da1e2a0b122023-11-22T19:44:48ZengMDPI AGPlants2223-77472021-10-011010215910.3390/plants10102159Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS SpectroscopyMiguel García-Parra0Diego Roa-Acosta1Víctor García-Londoño2Brigitte Moreno-Medina3Jesús Bravo-Gomez4Doctoral Program in Agriculture and Agroindustrial Science, Agriculture Department, Universidad del Cauca, Popayán 190002, ColombiaAgroindustry Department, Faculty of Agricultural Sciences, Universidad del Cauca, Popayán 190002, ColombiaInstitute of Polymer and Nanotechnology, Facultad de Arquitectura Diseño y Urbanismo, University of Buenos Aires-CONICET, Intendente Güiraldes 2160, Buenos Aires C1428EGA, ArgentinaFacultad de Ciencias Agropecuarias, Universidad Pedagógica y Tecnológica de Colombia (UPTC), Tunja 150002, ColombiaAgroindustry Department, Faculty of Agricultural Sciences, Universidad del Cauca, Popayán 190002, ColombiaThe existence of more of 16,000 varieties of quinoa accessions around the world has caused a disregard on their structural and phytochemical characteristics. Most of such accessions belong to cultivars settled in Colombia. The goal of this research was to evaluate the structural attributes and antioxidant capacities from six quinoa cultivars with high productive potential from central regions in Colombia. This study used middle-range infrared spectroscopy (IR-MIR) to determine the proteins, starch and lipids distinctive to quinoa grains. Ultra-high-performance liquid chromatography electrospray ionization Orbitrap, along with high-resolution mass spectrometry (UHPLC/ESI-Orbitrap MS), were also used to identify the existence of polyphenols in cultivars. The antioxidant capacity was determined through DPPH, ABTS and FRAP. The spectrums exhibited significant variances on the transmittance bands associated with 2922 cm<sup>−1</sup>, 1016 cm<sup>−1</sup> and 1633 cm<sup>−1</sup>. Moreover, the intensity variations on the peaks from the secondary protein structure were identified, mainly on the bands associated with β-Sheet-1 and -2, random coil α elice and β-turns-2 and -3. Changes found in the ratios 996 cm<sup>−1</sup>/1014 cm<sup>−1</sup> and 1041 cm<sup>−1</sup>/1014 cm<sup>−1</sup> were associated with the crystalline/amorphous affinity. Regarding the antioxidant capacity, great differences were identified (<i>p</i> < 0.001) mainly through FRAP methods, while the phenolic acids and flavonoids were determined by UHPLC/ESI-Orbitrap MS techniques. The presence of apigenin and pinocembrin on grains was reported for the first time. Titicaca and Nariño were the most phytochemically diverse quinoa seeds.https://www.mdpi.com/2223-7747/10/10/2159Amaranthaceaechromatographypolyphenolquinoa starchsecondary protein |
| spellingShingle | Miguel García-Parra Diego Roa-Acosta Víctor García-Londoño Brigitte Moreno-Medina Jesús Bravo-Gomez Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy Plants Amaranthaceae chromatography polyphenol quinoa starch secondary protein |
| title | Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy |
| title_full | Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy |
| title_fullStr | Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy |
| title_full_unstemmed | Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy |
| title_short | Structural Characterization and Antioxidant Capacity of Quinoa Cultivars Using Techniques of FT-MIR and UHPLC/ESI-Orbitrap MS Spectroscopy |
| title_sort | structural characterization and antioxidant capacity of quinoa cultivars using techniques of ft mir and uhplc esi orbitrap ms spectroscopy |
| topic | Amaranthaceae chromatography polyphenol quinoa starch secondary protein |
| url | https://www.mdpi.com/2223-7747/10/10/2159 |
| work_keys_str_mv | AT miguelgarciaparra structuralcharacterizationandantioxidantcapacityofquinoacultivarsusingtechniquesofftmiranduhplcesiorbitrapmsspectroscopy AT diegoroaacosta structuralcharacterizationandantioxidantcapacityofquinoacultivarsusingtechniquesofftmiranduhplcesiorbitrapmsspectroscopy AT victorgarcialondono structuralcharacterizationandantioxidantcapacityofquinoacultivarsusingtechniquesofftmiranduhplcesiorbitrapmsspectroscopy AT brigittemorenomedina structuralcharacterizationandantioxidantcapacityofquinoacultivarsusingtechniquesofftmiranduhplcesiorbitrapmsspectroscopy AT jesusbravogomez structuralcharacterizationandantioxidantcapacityofquinoacultivarsusingtechniquesofftmiranduhplcesiorbitrapmsspectroscopy |