Extract of Celosia leptostachya Leaves Inhibits Cobra Venom Toxins
Background: Celosia leptostachya belongs to Amaranthaceae plant family. Its leaves are used traditionally in the treatment of conditions, such as convulsion, eye infection and most notably to cure snakebites. This study investigated the inhibiting effect of the extract of C. leptostachya leaves agai...
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2023-05-01
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| Series: | Iranian Journal of Toxicology |
| Subjects: | |
| Online Access: | http://ijt.arakmu.ac.ir/article-1-1198-en.pdf |
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| author | Eugene O. Ohanme Godwin C. Akuodor Casimir C. Ofor Kenneth E. Etu Mansur A. Ramalan Donatus O. Anele Omotayo O. Erejuwa |
| author_facet | Eugene O. Ohanme Godwin C. Akuodor Casimir C. Ofor Kenneth E. Etu Mansur A. Ramalan Donatus O. Anele Omotayo O. Erejuwa |
| author_sort | Eugene O. Ohanme |
| collection | DOAJ |
| description | Background: Celosia leptostachya belongs to Amaranthaceae plant family. Its leaves are used traditionally in the treatment of conditions, such as convulsion, eye infection and most notably to cure snakebites. This study investigated the inhibiting effect of the extract of C. leptostachya leaves against cobra snake venom in mice.
Methods: We used 36 albino mice of mixed gender, weighing 20-25 g. They were divided into six groups of six rats each. Each rat was pre-treated with 0.4 mg/kg of cobra snake venom, and was subsequently given a graded dose of zero, 50, 100, 150, 200, or 250 mg/kg of the ethanol extract of C. leptostachya leaves. The animals were monitored for the survival rate. Various inhibition assays were performed to estimate the activities of acetyl cholinesterase, protease and adenosine triphosphatase of cobra venom, in the presence of 100-300 µg of the plant extract.
Results: The extract inhibited the effects of cobra venom significantly after the intraperitoneal injection of the venom and extract at 20 minutes intervals. The results demonstrated that 100% of the mice survived if they received 100-250 mg/kg of the extract while only 83.3% survived with the extract at 50 mg/kg. The extract inhibited the venom’s acetyl cholinesterase, protease and adenosine triphosphatase. The inhibition occurred at higher percentages if the extract was given at 300 mg/kg.
Conclusion: The plant extract significantly inhibited the snake venom, and its acetyl cholinesterase, protease and adenosine triphosphatase that mediated the venom’s toxicity. |
| first_indexed | 2024-03-13T03:03:01Z |
| format | Article |
| id | doaj.art-d52c0f8bcd17438598f6a1dc04dd6b8c |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-03-13T03:03:01Z |
| publishDate | 2023-05-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-d52c0f8bcd17438598f6a1dc04dd6b8c2023-06-27T10:58:20ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592023-05-01172127136Extract of Celosia leptostachya Leaves Inhibits Cobra Venom ToxinsEugene O. Ohanme0Godwin C. Akuodor1Casimir C. Ofor2Kenneth E. Etu3Mansur A. Ramalan4Donatus O. Anele5Omotayo O. Erejuwa6 Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, Alex Ekwueme Federal University, Ndifu- Alike, Ikwo, Ebonyi State Nigeria. Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nigeria. Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, Ebonyi State University, Abakaliki, Nigeria. Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, Alex Ekwueme Federal University, Ndifu- Alike, Ikwo, Ebonyi State Nigeria. Department of Pharmacology and Therapeutics, College of Health Sciences, Bayero University, Kano, Nigeria. Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, Abia State University, Uturu, Nigeria. Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, Alex Ekwueme Federal University, Ndifu- Alike, Ikwo, Ebonyi State Nigeria. Background: Celosia leptostachya belongs to Amaranthaceae plant family. Its leaves are used traditionally in the treatment of conditions, such as convulsion, eye infection and most notably to cure snakebites. This study investigated the inhibiting effect of the extract of C. leptostachya leaves against cobra snake venom in mice. Methods: We used 36 albino mice of mixed gender, weighing 20-25 g. They were divided into six groups of six rats each. Each rat was pre-treated with 0.4 mg/kg of cobra snake venom, and was subsequently given a graded dose of zero, 50, 100, 150, 200, or 250 mg/kg of the ethanol extract of C. leptostachya leaves. The animals were monitored for the survival rate. Various inhibition assays were performed to estimate the activities of acetyl cholinesterase, protease and adenosine triphosphatase of cobra venom, in the presence of 100-300 µg of the plant extract. Results: The extract inhibited the effects of cobra venom significantly after the intraperitoneal injection of the venom and extract at 20 minutes intervals. The results demonstrated that 100% of the mice survived if they received 100-250 mg/kg of the extract while only 83.3% survived with the extract at 50 mg/kg. The extract inhibited the venom’s acetyl cholinesterase, protease and adenosine triphosphatase. The inhibition occurred at higher percentages if the extract was given at 300 mg/kg. Conclusion: The plant extract significantly inhibited the snake venom, and its acetyl cholinesterase, protease and adenosine triphosphatase that mediated the venom’s toxicity.http://ijt.arakmu.ac.ir/article-1-1198-en.pdfanti-snake venomcelosia leptostachyaeffective doseinhibitory effect |
| spellingShingle | Eugene O. Ohanme Godwin C. Akuodor Casimir C. Ofor Kenneth E. Etu Mansur A. Ramalan Donatus O. Anele Omotayo O. Erejuwa Extract of Celosia leptostachya Leaves Inhibits Cobra Venom Toxins Iranian Journal of Toxicology anti-snake venom celosia leptostachya effective dose inhibitory effect |
| title | Extract of Celosia leptostachya Leaves Inhibits Cobra Venom Toxins |
| title_full | Extract of Celosia leptostachya Leaves Inhibits Cobra Venom Toxins |
| title_fullStr | Extract of Celosia leptostachya Leaves Inhibits Cobra Venom Toxins |
| title_full_unstemmed | Extract of Celosia leptostachya Leaves Inhibits Cobra Venom Toxins |
| title_short | Extract of Celosia leptostachya Leaves Inhibits Cobra Venom Toxins |
| title_sort | extract of celosia leptostachya leaves inhibits cobra venom toxins |
| topic | anti-snake venom celosia leptostachya effective dose inhibitory effect |
| url | http://ijt.arakmu.ac.ir/article-1-1198-en.pdf |
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