Simultaneous detection of Ca2+ and diacylglycerol signaling in living cells.
Phospholipase C produces two second messengers--diacylglycerol (DAG), which remains in the membrane, and inositol triphosphate (IP(3)), which triggers the release of calcium ions (Ca(2+)) from intracellular stores. Genetically encoded sensors based on a single circularly permuted fluorescent protein...
Main Authors: | Paul Tewson, Mara Westenberg, Yongxin Zhao, Robert E Campbell, Anne Marie Quinn, Thomas E Hughes |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3422227?pdf=render |
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