Streptococcus pneumoniae in saliva of Dutch primary school children.
While nasopharyngeal sampling is the gold standard for the detection of Streptococcus pneumoniae carriage, historically seen, saliva sampling also seems highly sensitive for pneumococcal detection. We investigated S. pneumoniae carriage in saliva from fifty schoolchildren by conventional and molecul...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4094488?pdf=render |
| _version_ | 1818933109932949504 |
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| author | Anne L Wyllie Mei Ling J N Chu Mariëlle H B Schellens Jody van Engelsdorp Gastelaars Marc D Jansen Arie van der Ende Debby Bogaert Elisabeth A M Sanders Krzysztof Trzciński |
| author_facet | Anne L Wyllie Mei Ling J N Chu Mariëlle H B Schellens Jody van Engelsdorp Gastelaars Marc D Jansen Arie van der Ende Debby Bogaert Elisabeth A M Sanders Krzysztof Trzciński |
| author_sort | Anne L Wyllie |
| collection | DOAJ |
| description | While nasopharyngeal sampling is the gold standard for the detection of Streptococcus pneumoniae carriage, historically seen, saliva sampling also seems highly sensitive for pneumococcal detection. We investigated S. pneumoniae carriage in saliva from fifty schoolchildren by conventional and molecular methods. Saliva was first culture-enriched for pneumococci, after which, DNA was extracted from all bacterial growth and tested by quantitative-PCR (qPCR) for pneumococcus-specific genes lytA and piaA. Next, serotype composition of the samples was determined by serotype-specific qPCRs, conventional-PCRs (cPCR) and sequencing of cPCR amplicons. Although only 2 (4%) of 50 samples were positive by conventional diagnostic culture, 44 (88%) were positive for pneumococci by qPCR. In total, we detected the presence of at least 81 pneumococcal strains representing 20 serotypes in samples from 44 carriers with 23 carriers (52%) positive for multiple (up to 6) serotypes. The number of serotypes detected per sample correlated with pneumococcal abundance. This study shows that saliva could be used as a tool for future pneumococcal surveillance studies. Furthermore, high rates of pneumococcal carriage and co-carriage of multiple pneumococcal strains together with a large number of serotypes in circulation suggests a ubiquitous presence of S. pneumoniae in saliva of school-aged children. Our results also suggest that factors promoting pneumococcal carriage within individual hosts may weaken competitive interactions between S. pneumoniae strains. |
| first_indexed | 2024-12-20T04:43:10Z |
| format | Article |
| id | doaj.art-d53b51b2aec24774af5e5c82ec951f19 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T04:43:10Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d53b51b2aec24774af5e5c82ec951f192022-12-21T19:53:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10204510.1371/journal.pone.0102045Streptococcus pneumoniae in saliva of Dutch primary school children.Anne L WyllieMei Ling J N ChuMariëlle H B SchellensJody van Engelsdorp GastelaarsMarc D JansenArie van der EndeDebby BogaertElisabeth A M SandersKrzysztof TrzcińskiWhile nasopharyngeal sampling is the gold standard for the detection of Streptococcus pneumoniae carriage, historically seen, saliva sampling also seems highly sensitive for pneumococcal detection. We investigated S. pneumoniae carriage in saliva from fifty schoolchildren by conventional and molecular methods. Saliva was first culture-enriched for pneumococci, after which, DNA was extracted from all bacterial growth and tested by quantitative-PCR (qPCR) for pneumococcus-specific genes lytA and piaA. Next, serotype composition of the samples was determined by serotype-specific qPCRs, conventional-PCRs (cPCR) and sequencing of cPCR amplicons. Although only 2 (4%) of 50 samples were positive by conventional diagnostic culture, 44 (88%) were positive for pneumococci by qPCR. In total, we detected the presence of at least 81 pneumococcal strains representing 20 serotypes in samples from 44 carriers with 23 carriers (52%) positive for multiple (up to 6) serotypes. The number of serotypes detected per sample correlated with pneumococcal abundance. This study shows that saliva could be used as a tool for future pneumococcal surveillance studies. Furthermore, high rates of pneumococcal carriage and co-carriage of multiple pneumococcal strains together with a large number of serotypes in circulation suggests a ubiquitous presence of S. pneumoniae in saliva of school-aged children. Our results also suggest that factors promoting pneumococcal carriage within individual hosts may weaken competitive interactions between S. pneumoniae strains.http://europepmc.org/articles/PMC4094488?pdf=render |
| spellingShingle | Anne L Wyllie Mei Ling J N Chu Mariëlle H B Schellens Jody van Engelsdorp Gastelaars Marc D Jansen Arie van der Ende Debby Bogaert Elisabeth A M Sanders Krzysztof Trzciński Streptococcus pneumoniae in saliva of Dutch primary school children. PLoS ONE |
| title | Streptococcus pneumoniae in saliva of Dutch primary school children. |
| title_full | Streptococcus pneumoniae in saliva of Dutch primary school children. |
| title_fullStr | Streptococcus pneumoniae in saliva of Dutch primary school children. |
| title_full_unstemmed | Streptococcus pneumoniae in saliva of Dutch primary school children. |
| title_short | Streptococcus pneumoniae in saliva of Dutch primary school children. |
| title_sort | streptococcus pneumoniae in saliva of dutch primary school children |
| url | http://europepmc.org/articles/PMC4094488?pdf=render |
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