Mechanism of Gynecological Tiaoqi Jiedu Formula in Treatment of Endometritis with Dampness and Heat: A Prospective Laboratory-Based Mice Model Study
Background: Endometritis is a common gynecological disease characterized by inflammation of the endometrium. The gynecological Tiaoqi Jiedu formula has been widely used to treat endometritis with dampness and heat. However, the mechanism of action remains unclear. Methods: A mouse model of endometri...
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| Format: | Article |
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IMR Press
2023-12-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/50/12/10.31083/j.ceog5012276 |
| _version_ | 1797365408269860864 |
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| author | Jixiang Chen Chuanmei Zhong Wensi Yu Yan Lv Ning Li |
| author_facet | Jixiang Chen Chuanmei Zhong Wensi Yu Yan Lv Ning Li |
| author_sort | Jixiang Chen |
| collection | DOAJ |
| description | Background: Endometritis is a common gynecological disease characterized by inflammation of the endometrium. The gynecological Tiaoqi Jiedu formula has been widely used to treat endometritis with dampness and heat. However, the mechanism of action remains unclear. Methods: A mouse model of endometritis with dampness and heat was established. The pathological changes of the uterus and tongue were detected by hematoxylin and eosin (HE) staining. Gastric aquaporin 3 (AQP3) and uterine cluster of differentiation 14 (CD14) protein were detected by immunohistochemistry. Concentrations of serum inflammatory factors interleukin-6 (IL-6), IL-1β, tumor necrosis factor α (TNF-α), IL-10, and IL-8 were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA levels and protein expressions of nucleotide binding oligomerization domain- (NOD-), leucine-rich repeat- (LRR-) and NOD-like receptor thermal protein domain associated protein 3 (NLRP3), gasdermin D (GSDMD), caspase-1, toll-like receptor 4 (TLR4), p65, and phosphorylation of p65 (p-p65) were determined by real-time polymerase chain reaction (PCR) and Western Blot, respectively. Results: In the mouse model of endometritis, the medium- and high-doses of Tiaoqi Jiedu formula, and western medicine significantly downregulated the inflammatory factors IL-6, IL-1β, TNF-α, and IL-8. It upregulated the anti-inflammatory factor IL-10, and inhibited pyroptosis and the expression of key proteins in the TLR4/nuclear factor-κB (NF-κB) signaling pathway. Conclusions: The Tiaoqi Jiedu formula demonstrated anti-inflammatory, anti-pyroptosis, and protective effects in endometritis. It has the potential to be a therapeutic option for the treatment of endometritis. |
| first_indexed | 2024-03-08T16:48:27Z |
| format | Article |
| id | doaj.art-d53ccf0b4e6c4f67bca424de2f4bb321 |
| institution | Directory Open Access Journal |
| issn | 0390-6663 |
| language | English |
| last_indexed | 2024-03-08T16:48:27Z |
| publishDate | 2023-12-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj.art-d53ccf0b4e6c4f67bca424de2f4bb3212024-01-05T07:27:09ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632023-12-01501227610.31083/j.ceog5012276S0390-6663(23)02184-XMechanism of Gynecological Tiaoqi Jiedu Formula in Treatment of Endometritis with Dampness and Heat: A Prospective Laboratory-Based Mice Model StudyJixiang Chen0Chuanmei Zhong1Wensi Yu2Yan Lv3Ning Li4Department of Obstetrics and Gynecology, Nanning Second Maternal and Child Health Hospital, 530001 Nanning, Guangxi, ChinaDepartment of Gynecology, Guangxi University of Chinese Medicine, 530001 Nanning, Guangxi, ChinaDepartment of Gynecology, Guangxi University of Chinese Medicine, 530001 Nanning, Guangxi, ChinaDepartment of Obstetrics and Gynecology, Songzi Maternal and Child Health Hospital, 434200 Jinzhou, Hubei, ChinaDepartment of Gynecology, Guangxi International Zhuang Medical Hospital Affiliated to Guangxi University of Chinese Medicine, 530001 Nanning, Guangxi, ChinaBackground: Endometritis is a common gynecological disease characterized by inflammation of the endometrium. The gynecological Tiaoqi Jiedu formula has been widely used to treat endometritis with dampness and heat. However, the mechanism of action remains unclear. Methods: A mouse model of endometritis with dampness and heat was established. The pathological changes of the uterus and tongue were detected by hematoxylin and eosin (HE) staining. Gastric aquaporin 3 (AQP3) and uterine cluster of differentiation 14 (CD14) protein were detected by immunohistochemistry. Concentrations of serum inflammatory factors interleukin-6 (IL-6), IL-1β, tumor necrosis factor α (TNF-α), IL-10, and IL-8 were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA levels and protein expressions of nucleotide binding oligomerization domain- (NOD-), leucine-rich repeat- (LRR-) and NOD-like receptor thermal protein domain associated protein 3 (NLRP3), gasdermin D (GSDMD), caspase-1, toll-like receptor 4 (TLR4), p65, and phosphorylation of p65 (p-p65) were determined by real-time polymerase chain reaction (PCR) and Western Blot, respectively. Results: In the mouse model of endometritis, the medium- and high-doses of Tiaoqi Jiedu formula, and western medicine significantly downregulated the inflammatory factors IL-6, IL-1β, TNF-α, and IL-8. It upregulated the anti-inflammatory factor IL-10, and inhibited pyroptosis and the expression of key proteins in the TLR4/nuclear factor-κB (NF-κB) signaling pathway. Conclusions: The Tiaoqi Jiedu formula demonstrated anti-inflammatory, anti-pyroptosis, and protective effects in endometritis. It has the potential to be a therapeutic option for the treatment of endometritis.https://www.imrpress.com/journal/CEOG/50/12/10.31083/j.ceog5012276endometritisgynecologytiaoqi jiedu formulalipopolysaccharideinflammatory factorsanimal studies |
| spellingShingle | Jixiang Chen Chuanmei Zhong Wensi Yu Yan Lv Ning Li Mechanism of Gynecological Tiaoqi Jiedu Formula in Treatment of Endometritis with Dampness and Heat: A Prospective Laboratory-Based Mice Model Study Clinical and Experimental Obstetrics & Gynecology endometritis gynecology tiaoqi jiedu formula lipopolysaccharide inflammatory factors animal studies |
| title | Mechanism of Gynecological Tiaoqi Jiedu Formula in Treatment of Endometritis with Dampness and Heat: A Prospective Laboratory-Based Mice Model Study |
| title_full | Mechanism of Gynecological Tiaoqi Jiedu Formula in Treatment of Endometritis with Dampness and Heat: A Prospective Laboratory-Based Mice Model Study |
| title_fullStr | Mechanism of Gynecological Tiaoqi Jiedu Formula in Treatment of Endometritis with Dampness and Heat: A Prospective Laboratory-Based Mice Model Study |
| title_full_unstemmed | Mechanism of Gynecological Tiaoqi Jiedu Formula in Treatment of Endometritis with Dampness and Heat: A Prospective Laboratory-Based Mice Model Study |
| title_short | Mechanism of Gynecological Tiaoqi Jiedu Formula in Treatment of Endometritis with Dampness and Heat: A Prospective Laboratory-Based Mice Model Study |
| title_sort | mechanism of gynecological tiaoqi jiedu formula in treatment of endometritis with dampness and heat a prospective laboratory based mice model study |
| topic | endometritis gynecology tiaoqi jiedu formula lipopolysaccharide inflammatory factors animal studies |
| url | https://www.imrpress.com/journal/CEOG/50/12/10.31083/j.ceog5012276 |
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