MiR-219-5p suppresses cell proliferation and cell cycle progression in esophageal squamous cell carcinoma by targeting CCNA2
Abstract Background We investigated the potential regulatory role of miR-219-5p in esophageal squamous cell carcinoma (ESCC) and looked at the underlying mechanisms in ESCC. Methods Real-time PCR was used to determine the levels of miR-219-5p in ESCC tissues and cell lines. The effects of miR-219-5p...
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | Cellular & Molecular Biology Letters |
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| Online Access: | http://link.springer.com/article/10.1186/s11658-018-0129-6 |
| _version_ | 1830297227188240384 |
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| author | Qiang Ma |
| author_facet | Qiang Ma |
| author_sort | Qiang Ma |
| collection | DOAJ |
| description | Abstract Background We investigated the potential regulatory role of miR-219-5p in esophageal squamous cell carcinoma (ESCC) and looked at the underlying mechanisms in ESCC. Methods Real-time PCR was used to determine the levels of miR-219-5p in ESCC tissues and cell lines. The effects of miR-219-5p and cyclin A2 (CCNA2) on cell proliferation and cell cycle progression were evaluated using MTT, colony formation and flow cytometry assays with ESCC cell lines EC9706 and TE-9. Bioinformatics techniques and the luciferase reporter assay were applied to validate CCNA2 as the miR-219-5p target in ESCC cells. The mRNA and protein levels of CCNA2 were measured using real-time PCR and western blotting. Results MiR-219-5p expression was significantly lower in ESCC tissues and cells than in healthy tissues. Upregulation of miR-219-5p repressed cell proliferation and induced cell cycle arrest at the G2/M phase. CCNA2 was identified and confirmed as a direct downstream target of miR-219-5p and its expression negatively correlated with miR-219-5p profiles in ESCC tissues. Knockdown of CCNA2 potentiated the effects of miR-219-5p on cell proliferation and cell cycle distribution. Conclusions Our results demonstrate that miR-219-5p might function as a tumor suppressor by directly targeting CCNA2 expression. It could serve as a new therapeutic target for ESCC. |
| first_indexed | 2024-12-19T07:29:16Z |
| format | Article |
| id | doaj.art-d5429af927c74401a3ea6181eed03ef5 |
| institution | Directory Open Access Journal |
| issn | 1425-8153 1689-1392 |
| language | English |
| last_indexed | 2024-12-19T07:29:16Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Cellular & Molecular Biology Letters |
| spelling | doaj.art-d5429af927c74401a3ea6181eed03ef52022-12-21T20:30:45ZengBMCCellular & Molecular Biology Letters1425-81531689-13922019-02-0124111310.1186/s11658-018-0129-6MiR-219-5p suppresses cell proliferation and cell cycle progression in esophageal squamous cell carcinoma by targeting CCNA2Qiang Ma0Department of Oncology, People’s Hospital of Xintai CityAbstract Background We investigated the potential regulatory role of miR-219-5p in esophageal squamous cell carcinoma (ESCC) and looked at the underlying mechanisms in ESCC. Methods Real-time PCR was used to determine the levels of miR-219-5p in ESCC tissues and cell lines. The effects of miR-219-5p and cyclin A2 (CCNA2) on cell proliferation and cell cycle progression were evaluated using MTT, colony formation and flow cytometry assays with ESCC cell lines EC9706 and TE-9. Bioinformatics techniques and the luciferase reporter assay were applied to validate CCNA2 as the miR-219-5p target in ESCC cells. The mRNA and protein levels of CCNA2 were measured using real-time PCR and western blotting. Results MiR-219-5p expression was significantly lower in ESCC tissues and cells than in healthy tissues. Upregulation of miR-219-5p repressed cell proliferation and induced cell cycle arrest at the G2/M phase. CCNA2 was identified and confirmed as a direct downstream target of miR-219-5p and its expression negatively correlated with miR-219-5p profiles in ESCC tissues. Knockdown of CCNA2 potentiated the effects of miR-219-5p on cell proliferation and cell cycle distribution. Conclusions Our results demonstrate that miR-219-5p might function as a tumor suppressor by directly targeting CCNA2 expression. It could serve as a new therapeutic target for ESCC.http://link.springer.com/article/10.1186/s11658-018-0129-6Esophageal squamous cell carcinomamiR-219-5pCCNA2Cell proliferationG2/M phase arrest |
| spellingShingle | Qiang Ma MiR-219-5p suppresses cell proliferation and cell cycle progression in esophageal squamous cell carcinoma by targeting CCNA2 Cellular & Molecular Biology Letters Esophageal squamous cell carcinoma miR-219-5p CCNA2 Cell proliferation G2/M phase arrest |
| title | MiR-219-5p suppresses cell proliferation and cell cycle progression in esophageal squamous cell carcinoma by targeting CCNA2 |
| title_full | MiR-219-5p suppresses cell proliferation and cell cycle progression in esophageal squamous cell carcinoma by targeting CCNA2 |
| title_fullStr | MiR-219-5p suppresses cell proliferation and cell cycle progression in esophageal squamous cell carcinoma by targeting CCNA2 |
| title_full_unstemmed | MiR-219-5p suppresses cell proliferation and cell cycle progression in esophageal squamous cell carcinoma by targeting CCNA2 |
| title_short | MiR-219-5p suppresses cell proliferation and cell cycle progression in esophageal squamous cell carcinoma by targeting CCNA2 |
| title_sort | mir 219 5p suppresses cell proliferation and cell cycle progression in esophageal squamous cell carcinoma by targeting ccna2 |
| topic | Esophageal squamous cell carcinoma miR-219-5p CCNA2 Cell proliferation G2/M phase arrest |
| url | http://link.springer.com/article/10.1186/s11658-018-0129-6 |
| work_keys_str_mv | AT qiangma mir2195psuppressescellproliferationandcellcycleprogressioninesophagealsquamouscellcarcinomabytargetingccna2 |