The Blood Cytokine Profile of Young People with Early Ischemic Heart Disease Comorbid with Abdominal Obesity
Objective: The aim was to study the blood cytokine/chemokine profile of 25–44-year-old people with early ischemic heart disease (IHD) comorbid with abdominal obesity (AO). Methods: A cross-sectional medical examination of subjects in Novosibirsk, Russia, was conducted after random sampling of the ab...
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2020-08-01
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author | Yulia I. Ragino Veronika I. Oblaukhova Yana V. Polonskaya Natalya A. Kuzminykh Liliya V. Shcherbakova Elena V. Kashtanova |
author_facet | Yulia I. Ragino Veronika I. Oblaukhova Yana V. Polonskaya Natalya A. Kuzminykh Liliya V. Shcherbakova Elena V. Kashtanova |
author_sort | Yulia I. Ragino |
collection | DOAJ |
description | Objective: The aim was to study the blood cytokine/chemokine profile of 25–44-year-old people with early ischemic heart disease (IHD) comorbid with abdominal obesity (AO). Methods: A cross-sectional medical examination of subjects in Novosibirsk, Russia, was conducted after random sampling of the above age group. A total of 1457 subjects, 804 females and 653 males, were analyzed. The epidemiological diagnosis of IHD was made in accordance with 17 validated and functional criteria, employing exercise ECG for confirmation. Simultaneous quantitative analyses of 41 cytokines/chemokines in blood serum were performed by a multiplex assay using the HCYTMAG-60K-PX41 panel (MILLIPLEX MAP) on a Luminex 20 MAGPIX flow cytometer, with additional ELISA testing. Results: Flt3 ligand, GM-CSF, and MCP-1 were significantly associated with the relative risk of early IHD. In the presence of AO, GM-CSF, MCP-1 and IL-4 also significantly correlated with the relative risk of early IHD. By univariate regression analysis, the relative risk of early IHD was associated with lowered blood concentrations of Flt3 ligand, whereas the relative risk of early IHD in the presence of AO was associated with lowered blood concentrations of GM-CSF. Employing multivariable regression analysis, only lower blood levels of Flt3 ligand were associated with a relative risk of early IHD, whereas the relative risk of early IHD in the presence of AO was limited to lower levels of IL-4. Conclusion: Findings related to Flt3 ligand, GM-CSF, and IL-4 are consistent with the international literature. Results from the present study are partly confirmative and partly hypothesis generating. |
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spelling | doaj.art-d54430bec91e477f9653e5f04f8035e02023-11-20T10:02:40ZengMDPI AGJournal of Personalized Medicine2075-44262020-08-011038710.3390/jpm10030087The Blood Cytokine Profile of Young People with Early Ischemic Heart Disease Comorbid with Abdominal ObesityYulia I. Ragino0Veronika I. Oblaukhova1Yana V. Polonskaya2Natalya A. Kuzminykh3Liliya V. Shcherbakova4Elena V. Kashtanova5Research Institute of Internal and Preventive Medicine—Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (IIPM–Branch of IC&G SB RAS), 175/1 B. Bogatkova Str., 630089 Novosibirsk, RussiaResearch Institute of Internal and Preventive Medicine—Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (IIPM–Branch of IC&G SB RAS), 175/1 B. Bogatkova Str., 630089 Novosibirsk, RussiaResearch Institute of Internal and Preventive Medicine—Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (IIPM–Branch of IC&G SB RAS), 175/1 B. Bogatkova Str., 630089 Novosibirsk, RussiaResearch Institute of Internal and Preventive Medicine—Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (IIPM–Branch of IC&G SB RAS), 175/1 B. Bogatkova Str., 630089 Novosibirsk, RussiaResearch Institute of Internal and Preventive Medicine—Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (IIPM–Branch of IC&G SB RAS), 175/1 B. Bogatkova Str., 630089 Novosibirsk, RussiaResearch Institute of Internal and Preventive Medicine—Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (IIPM–Branch of IC&G SB RAS), 175/1 B. Bogatkova Str., 630089 Novosibirsk, RussiaObjective: The aim was to study the blood cytokine/chemokine profile of 25–44-year-old people with early ischemic heart disease (IHD) comorbid with abdominal obesity (AO). Methods: A cross-sectional medical examination of subjects in Novosibirsk, Russia, was conducted after random sampling of the above age group. A total of 1457 subjects, 804 females and 653 males, were analyzed. The epidemiological diagnosis of IHD was made in accordance with 17 validated and functional criteria, employing exercise ECG for confirmation. Simultaneous quantitative analyses of 41 cytokines/chemokines in blood serum were performed by a multiplex assay using the HCYTMAG-60K-PX41 panel (MILLIPLEX MAP) on a Luminex 20 MAGPIX flow cytometer, with additional ELISA testing. Results: Flt3 ligand, GM-CSF, and MCP-1 were significantly associated with the relative risk of early IHD. In the presence of AO, GM-CSF, MCP-1 and IL-4 also significantly correlated with the relative risk of early IHD. By univariate regression analysis, the relative risk of early IHD was associated with lowered blood concentrations of Flt3 ligand, whereas the relative risk of early IHD in the presence of AO was associated with lowered blood concentrations of GM-CSF. Employing multivariable regression analysis, only lower blood levels of Flt3 ligand were associated with a relative risk of early IHD, whereas the relative risk of early IHD in the presence of AO was limited to lower levels of IL-4. Conclusion: Findings related to Flt3 ligand, GM-CSF, and IL-4 are consistent with the international literature. Results from the present study are partly confirmative and partly hypothesis generating.https://www.mdpi.com/2075-4426/10/3/87early IHDabdominal obesityblood cytokines/chemokines complexmultiplex assayFlt3 ligandGM-CSF |
spellingShingle | Yulia I. Ragino Veronika I. Oblaukhova Yana V. Polonskaya Natalya A. Kuzminykh Liliya V. Shcherbakova Elena V. Kashtanova The Blood Cytokine Profile of Young People with Early Ischemic Heart Disease Comorbid with Abdominal Obesity Journal of Personalized Medicine early IHD abdominal obesity blood cytokines/chemokines complex multiplex assay Flt3 ligand GM-CSF |
title | The Blood Cytokine Profile of Young People with Early Ischemic Heart Disease Comorbid with Abdominal Obesity |
title_full | The Blood Cytokine Profile of Young People with Early Ischemic Heart Disease Comorbid with Abdominal Obesity |
title_fullStr | The Blood Cytokine Profile of Young People with Early Ischemic Heart Disease Comorbid with Abdominal Obesity |
title_full_unstemmed | The Blood Cytokine Profile of Young People with Early Ischemic Heart Disease Comorbid with Abdominal Obesity |
title_short | The Blood Cytokine Profile of Young People with Early Ischemic Heart Disease Comorbid with Abdominal Obesity |
title_sort | blood cytokine profile of young people with early ischemic heart disease comorbid with abdominal obesity |
topic | early IHD abdominal obesity blood cytokines/chemokines complex multiplex assay Flt3 ligand GM-CSF |
url | https://www.mdpi.com/2075-4426/10/3/87 |
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