Response of Oxidative Stress and Antioxidant System in Pea Plants Exposed to Drought and Boron Nanoparticles
Pea plants are sensitive to water shortages, making them less attractive to farmers. Hoping to reduce the adverse effects of drought on peas and considering the benefits of boron, this study aimed to investigate the impact of boron nanoparticles on the antioxidant system and oxidative stress biomark...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/2/528 |
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| author | Rūta Sutulienė Aušra Brazaitytė Stanisław Małek Michał Jasik Giedrė Samuolienė |
| author_facet | Rūta Sutulienė Aušra Brazaitytė Stanisław Małek Michał Jasik Giedrė Samuolienė |
| author_sort | Rūta Sutulienė |
| collection | DOAJ |
| description | Pea plants are sensitive to water shortages, making them less attractive to farmers. Hoping to reduce the adverse effects of drought on peas and considering the benefits of boron, this study aimed to investigate the impact of boron nanoparticles on the antioxidant system and oxidative stress biomarkers in drought-stressed peas. Experiments were performed in a greenhouse. Pea plants were treated with a suspension of B<sub>2</sub>O<sub>3</sub> nanoparticles at 12.5, 25, and 50 ppm concentrations before ten days of water shortage. Drought effects were induced by maintaining 30% substrate moisture. This study investigated the properties of the nanoparticle suspension and different application methods for spraying and watering pea plants. The effects of B<sub>2</sub>O<sub>3</sub> nanoparticles and drought were determined on pea growth indicators, oxidative stress biomarkers, and enzymatic and non-enzymatic antioxidants. Spraying with B<sub>2</sub>O<sub>3</sub> nanoparticles at 12.5 ppm most effectively stimulated phenol accumulation; FRAP, DPPH, and ABTS antioxidant capacity; and APX, SOD, GPX, and CAT enzyme activity in pea leaves exposed to drought. In addition, B<sub>2</sub>O<sub>3</sub> nanoparticles reduced the amount of MDA and H<sub>2</sub>O<sub>2</sub> in pea plants grown on a substrate with insufficient moisture. The most substantial positive effect was found on peas affected by drought after spraying them with 12.5 ppm of B<sub>2</sub>O<sub>3</sub> nanoparticles. B<sub>2</sub>O<sub>3</sub> nanoparticles positively affected the pea height, leaf area, number of nodules, and yield. |
| first_indexed | 2024-03-11T09:14:24Z |
| format | Article |
| id | doaj.art-d544b612bfdc42778a2d0e8e1201de03 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T09:14:24Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-d544b612bfdc42778a2d0e8e1201de032023-11-16T18:49:31ZengMDPI AGAntioxidants2076-39212023-02-0112252810.3390/antiox12020528Response of Oxidative Stress and Antioxidant System in Pea Plants Exposed to Drought and Boron NanoparticlesRūta Sutulienė0Aušra Brazaitytė1Stanisław Małek2Michał Jasik3Giedrė Samuolienė4Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, Kaunas District, LT-54333 Babtai, LithuaniaInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, Kaunas District, LT-54333 Babtai, LithuaniaDepartment of Ecology and Silviculture, Faculty of Forestry, University of Agriculture in Krakow, Al. 29-go Listopada 46, 31-425 Kraków, PolandDepartment of Ecology and Silviculture, Faculty of Forestry, University of Agriculture in Krakow, Al. 29-go Listopada 46, 31-425 Kraków, PolandInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, Kaunas District, LT-54333 Babtai, LithuaniaPea plants are sensitive to water shortages, making them less attractive to farmers. Hoping to reduce the adverse effects of drought on peas and considering the benefits of boron, this study aimed to investigate the impact of boron nanoparticles on the antioxidant system and oxidative stress biomarkers in drought-stressed peas. Experiments were performed in a greenhouse. Pea plants were treated with a suspension of B<sub>2</sub>O<sub>3</sub> nanoparticles at 12.5, 25, and 50 ppm concentrations before ten days of water shortage. Drought effects were induced by maintaining 30% substrate moisture. This study investigated the properties of the nanoparticle suspension and different application methods for spraying and watering pea plants. The effects of B<sub>2</sub>O<sub>3</sub> nanoparticles and drought were determined on pea growth indicators, oxidative stress biomarkers, and enzymatic and non-enzymatic antioxidants. Spraying with B<sub>2</sub>O<sub>3</sub> nanoparticles at 12.5 ppm most effectively stimulated phenol accumulation; FRAP, DPPH, and ABTS antioxidant capacity; and APX, SOD, GPX, and CAT enzyme activity in pea leaves exposed to drought. In addition, B<sub>2</sub>O<sub>3</sub> nanoparticles reduced the amount of MDA and H<sub>2</sub>O<sub>2</sub> in pea plants grown on a substrate with insufficient moisture. The most substantial positive effect was found on peas affected by drought after spraying them with 12.5 ppm of B<sub>2</sub>O<sub>3</sub> nanoparticles. B<sub>2</sub>O<sub>3</sub> nanoparticles positively affected the pea height, leaf area, number of nodules, and yield.https://www.mdpi.com/2076-3921/12/2/528peadroughtboronnanoparticlesantioxidantsoxidative stress |
| spellingShingle | Rūta Sutulienė Aušra Brazaitytė Stanisław Małek Michał Jasik Giedrė Samuolienė Response of Oxidative Stress and Antioxidant System in Pea Plants Exposed to Drought and Boron Nanoparticles Antioxidants pea drought boron nanoparticles antioxidants oxidative stress |
| title | Response of Oxidative Stress and Antioxidant System in Pea Plants Exposed to Drought and Boron Nanoparticles |
| title_full | Response of Oxidative Stress and Antioxidant System in Pea Plants Exposed to Drought and Boron Nanoparticles |
| title_fullStr | Response of Oxidative Stress and Antioxidant System in Pea Plants Exposed to Drought and Boron Nanoparticles |
| title_full_unstemmed | Response of Oxidative Stress and Antioxidant System in Pea Plants Exposed to Drought and Boron Nanoparticles |
| title_short | Response of Oxidative Stress and Antioxidant System in Pea Plants Exposed to Drought and Boron Nanoparticles |
| title_sort | response of oxidative stress and antioxidant system in pea plants exposed to drought and boron nanoparticles |
| topic | pea drought boron nanoparticles antioxidants oxidative stress |
| url | https://www.mdpi.com/2076-3921/12/2/528 |
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