| Summary: | <i>Salvia corrugata</i> Vahl. is an interesting source of abietane and <i>abeo</i>-abietane compounds that showed antibacterial, antitumor, and cytotoxic activities. The aim of the study was to obtain transformed roots of <i>S. corrugata</i> and to evaluate the production of terpenoids in comparison with in vivo root production. Hairy roots were initiated from leaf explants by infection with ATCC 15834 <i>Agrobacterium rhizogenes</i> onto hormone-free Murashige and Skoog (MS) solid medium. Transformation was confirmed by polymerase chain reaction analysis of <i>rolC</i> and <i>virC1</i> genes. The biomass production was obtained in hormone-free liquid MS medium using Temporary Immersion System bioreactor RITA<sup>®</sup>. The chromatographic separation of the methanolic extract of the untransformed roots afforded horminone, ferruginol, 7-<i>O</i>-acetylhorminone and 7-<i>O</i>-methylhorminone. Agastol and ferruginol were isolated and quantified from the hairy roots. The amount of these metabolites indicated that the hairy roots of <i>S. corrugata</i> can be considered a source of these compounds.
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