Abietane Diterpenoids from the Hairy Roots of <i>Salvia corrugata</i>
<i>Salvia corrugata</i> Vahl. is an interesting source of abietane and <i>abeo</i>-abietane compounds that showed antibacterial, antitumor, and cytotoxic activities. The aim of the study was to obtain transformed roots of <i>S. corrugata</i> and to evaluate the pr...
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MDPI AG
2021-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/17/5144 |
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| author | Roméo Arago Dougué Kentsop Valeria Iobbi Giuliana Donadio Barbara Ruffoni Nunziatina De Tommasi Angela Bisio |
| author_facet | Roméo Arago Dougué Kentsop Valeria Iobbi Giuliana Donadio Barbara Ruffoni Nunziatina De Tommasi Angela Bisio |
| author_sort | Roméo Arago Dougué Kentsop |
| collection | DOAJ |
| description | <i>Salvia corrugata</i> Vahl. is an interesting source of abietane and <i>abeo</i>-abietane compounds that showed antibacterial, antitumor, and cytotoxic activities. The aim of the study was to obtain transformed roots of <i>S. corrugata</i> and to evaluate the production of terpenoids in comparison with in vivo root production. Hairy roots were initiated from leaf explants by infection with ATCC 15834 <i>Agrobacterium rhizogenes</i> onto hormone-free Murashige and Skoog (MS) solid medium. Transformation was confirmed by polymerase chain reaction analysis of <i>rolC</i> and <i>virC1</i> genes. The biomass production was obtained in hormone-free liquid MS medium using Temporary Immersion System bioreactor RITA<sup>®</sup>. The chromatographic separation of the methanolic extract of the untransformed roots afforded horminone, ferruginol, 7-<i>O</i>-acetylhorminone and 7-<i>O</i>-methylhorminone. Agastol and ferruginol were isolated and quantified from the hairy roots. The amount of these metabolites indicated that the hairy roots of <i>S. corrugata</i> can be considered a source of these compounds. |
| first_indexed | 2024-03-10T08:07:18Z |
| format | Article |
| id | doaj.art-d549116e1dce4495942a306a0a6b8981 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:07:18Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d549116e1dce4495942a306a0a6b89812023-11-22T10:59:47ZengMDPI AGMolecules1420-30492021-08-012617514410.3390/molecules26175144Abietane Diterpenoids from the Hairy Roots of <i>Salvia corrugata</i>Roméo Arago Dougué Kentsop0Valeria Iobbi1Giuliana Donadio2Barbara Ruffoni3Nunziatina De Tommasi4Angela Bisio5Dipartimento di Farmacia, Università di Genova, Viale Cembrano 4, 16148 Genova, ItalyDipartimento di Farmacia, Università di Genova, Viale Cembrano 4, 16148 Genova, ItalyDipartimento di Farmacia, Università di Salerno, Via Giovanni Paolo II 132, 84084 Salerno, ItalyConsiglio per la Ricerca e la Sperimentazione in Agricoltura—CREA Centro di Ricerca Orticoltura e Florovivaismo, Corso degli Inglesi, 508, 18038 Sanremo, ItalyDipartimento di Farmacia, Università di Salerno, Via Giovanni Paolo II 132, 84084 Salerno, ItalyDipartimento di Farmacia, Università di Genova, Viale Cembrano 4, 16148 Genova, Italy<i>Salvia corrugata</i> Vahl. is an interesting source of abietane and <i>abeo</i>-abietane compounds that showed antibacterial, antitumor, and cytotoxic activities. The aim of the study was to obtain transformed roots of <i>S. corrugata</i> and to evaluate the production of terpenoids in comparison with in vivo root production. Hairy roots were initiated from leaf explants by infection with ATCC 15834 <i>Agrobacterium rhizogenes</i> onto hormone-free Murashige and Skoog (MS) solid medium. Transformation was confirmed by polymerase chain reaction analysis of <i>rolC</i> and <i>virC1</i> genes. The biomass production was obtained in hormone-free liquid MS medium using Temporary Immersion System bioreactor RITA<sup>®</sup>. The chromatographic separation of the methanolic extract of the untransformed roots afforded horminone, ferruginol, 7-<i>O</i>-acetylhorminone and 7-<i>O</i>-methylhorminone. Agastol and ferruginol were isolated and quantified from the hairy roots. The amount of these metabolites indicated that the hairy roots of <i>S. corrugata</i> can be considered a source of these compounds.https://www.mdpi.com/1420-3049/26/17/5144in vitro tissue culturetemporary immersion system (TIS)abietane diterpenoidsferruginolagastol |
| spellingShingle | Roméo Arago Dougué Kentsop Valeria Iobbi Giuliana Donadio Barbara Ruffoni Nunziatina De Tommasi Angela Bisio Abietane Diterpenoids from the Hairy Roots of <i>Salvia corrugata</i> Molecules in vitro tissue culture temporary immersion system (TIS) abietane diterpenoids ferruginol agastol |
| title | Abietane Diterpenoids from the Hairy Roots of <i>Salvia corrugata</i> |
| title_full | Abietane Diterpenoids from the Hairy Roots of <i>Salvia corrugata</i> |
| title_fullStr | Abietane Diterpenoids from the Hairy Roots of <i>Salvia corrugata</i> |
| title_full_unstemmed | Abietane Diterpenoids from the Hairy Roots of <i>Salvia corrugata</i> |
| title_short | Abietane Diterpenoids from the Hairy Roots of <i>Salvia corrugata</i> |
| title_sort | abietane diterpenoids from the hairy roots of i salvia corrugata i |
| topic | in vitro tissue culture temporary immersion system (TIS) abietane diterpenoids ferruginol agastol |
| url | https://www.mdpi.com/1420-3049/26/17/5144 |
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