Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism
Summary Pichia pastoris KM71H (MutS) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP‐powered efflux pump which is expressed properly, but at very low concentration, using the conventional induction strategy. Evaluation of different induc...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-11-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13420 |
| _version_ | 1819080757996421120 |
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| author | Wan‐cang Liu Fei Zhou Di Xia Joseph Shiloach |
| author_facet | Wan‐cang Liu Fei Zhou Di Xia Joseph Shiloach |
| author_sort | Wan‐cang Liu |
| collection | DOAJ |
| description | Summary Pichia pastoris KM71H (MutS) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP‐powered efflux pump which is expressed properly, but at very low concentration, using the conventional induction strategy. Evaluation of different induction strategies indicated that it was possible to increase Pgp expression by inducing the culture with 20% media containing 2.5% methanol. By quantifying methanol, formaldehyde, hydrogen peroxide and formate, and by measuring alcohol oxidase, catalase, formaldehyde dehydrogenase, formate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase and α‐ketoglutarate dehydrogenases, it was possible to correlate Pgp expression to the induction strategy. Inducing the culture by adding methanol with fresh media was associated with decreases in formaldehyde and hydrogen peroxide, and increases in formaldehyde dehydrogenase, formate dehydrogenase, isocitrate dehydrogenase and α‐ketoglutarate dehydrogenases. At these conditions, Pgp expression was 1400‐fold higher, an indication that Pgp expression is affected by increases in formaldehyde and hydrogen peroxide. It is possible that Pgp is responsible for this behaviour, since the increased metabolite concentrations and decreased enzymatic activities were not observed when parental Pichia was subjected to the same growth conditions. This report adds information on methanol metabolism during expression of Pgp from P. pastoris MutS strain and suggests an expression procedure for hard‐to‐express proteins from P. pastoris. |
| first_indexed | 2024-12-21T19:49:58Z |
| format | Article |
| id | doaj.art-d54b4b6ab4c74d93ab7c38ccec71f4c1 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-21T19:49:58Z |
| publishDate | 2019-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-d54b4b6ab4c74d93ab7c38ccec71f4c12022-12-21T18:52:14ZengWileyMicrobial Biotechnology1751-79152019-11-011261226123610.1111/1751-7915.13420Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolismWan‐cang Liu0Fei Zhou1Di Xia2Joseph Shiloach3Biotechnology Core Laboratory National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) National Institutes of Health (NIH) Bethesda MD 20892 USALaboratory of Cell Biology Center for Cancer Research (CCR) National Cancer Institute (NCI) National Institutes of Health (NIH) Bethesda MD 20892 USALaboratory of Cell Biology Center for Cancer Research (CCR) National Cancer Institute (NCI) National Institutes of Health (NIH) Bethesda MD 20892 USABiotechnology Core Laboratory National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) National Institutes of Health (NIH) Bethesda MD 20892 USASummary Pichia pastoris KM71H (MutS) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP‐powered efflux pump which is expressed properly, but at very low concentration, using the conventional induction strategy. Evaluation of different induction strategies indicated that it was possible to increase Pgp expression by inducing the culture with 20% media containing 2.5% methanol. By quantifying methanol, formaldehyde, hydrogen peroxide and formate, and by measuring alcohol oxidase, catalase, formaldehyde dehydrogenase, formate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase and α‐ketoglutarate dehydrogenases, it was possible to correlate Pgp expression to the induction strategy. Inducing the culture by adding methanol with fresh media was associated with decreases in formaldehyde and hydrogen peroxide, and increases in formaldehyde dehydrogenase, formate dehydrogenase, isocitrate dehydrogenase and α‐ketoglutarate dehydrogenases. At these conditions, Pgp expression was 1400‐fold higher, an indication that Pgp expression is affected by increases in formaldehyde and hydrogen peroxide. It is possible that Pgp is responsible for this behaviour, since the increased metabolite concentrations and decreased enzymatic activities were not observed when parental Pichia was subjected to the same growth conditions. This report adds information on methanol metabolism during expression of Pgp from P. pastoris MutS strain and suggests an expression procedure for hard‐to‐express proteins from P. pastoris.https://doi.org/10.1111/1751-7915.13420 |
| spellingShingle | Wan‐cang Liu Fei Zhou Di Xia Joseph Shiloach Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism Microbial Biotechnology |
| title | Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism |
| title_full | Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism |
| title_fullStr | Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism |
| title_full_unstemmed | Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism |
| title_short | Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism |
| title_sort | expression of multidrug transporter p glycoprotein in pichia pastoris affects the host s methanol metabolism |
| url | https://doi.org/10.1111/1751-7915.13420 |
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