Selection against BALB/c strain cells in mouse chimaeras
It has been shown previously that BALB/c strain embryos tend to contribute poorly to mouse aggregation chimaeras. In the present study we showed that BALB/c cells were not preferentially allocated to any extraembryonic lineages of mouse aggregation chimaeras, but their contribution decreased during...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2018-01-01
|
| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/7/1/bio030189 |
| _version_ | 1818941342768693248 |
|---|---|
| author | Pin-Chi Tang Gillian E. MacKay Jean H. Flockhart Margaret A. Keighren Anna Kopakaki John D. West |
| author_facet | Pin-Chi Tang Gillian E. MacKay Jean H. Flockhart Margaret A. Keighren Anna Kopakaki John D. West |
| author_sort | Pin-Chi Tang |
| collection | DOAJ |
| description | It has been shown previously that BALB/c strain embryos tend to contribute poorly to mouse aggregation chimaeras. In the present study we showed that BALB/c cells were not preferentially allocated to any extraembryonic lineages of mouse aggregation chimaeras, but their contribution decreased during the early postimplantation period and they were significantly depleted by E8.5. The development of BALB/c strain preimplantation embryos lagged behind embryos from some other strains and the contribution that BALB/c and other embryos made to chimaeras correlated with their developmental stage at E2.5. This relationship suggests that the poor contribution of BALB/c embryos to aggregation chimaeras is at least partly a consequence of generalised selection related to slow or delayed preimplantation development. The suitability of BALB/c embryos for maximising the ES cell contribution to mouse ES cell chimaeras is also discussed. |
| first_indexed | 2024-12-20T06:54:01Z |
| format | Article |
| id | doaj.art-d54e671682e74f508a1721de9e029a0e |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-20T06:54:01Z |
| publishDate | 2018-01-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-d54e671682e74f508a1721de9e029a0e2022-12-21T19:49:25ZengThe Company of BiologistsBiology Open2046-63902018-01-017110.1242/bio.030189030189Selection against BALB/c strain cells in mouse chimaerasPin-Chi Tang0Gillian E. MacKay1Jean H. Flockhart2Margaret A. Keighren3Anna Kopakaki4John D. West5 Genes and Development Group, Centre for Integrative Physiology, Clinical Sciences, University of Edinburgh Medical School, Hugh Robson Building, George Square, Edinburgh, EH8 9XD, UK Genes and Development Group, Centre for Integrative Physiology, Clinical Sciences, University of Edinburgh Medical School, Hugh Robson Building, George Square, Edinburgh, EH8 9XD, UK Genes and Development Group, Centre for Integrative Physiology, Clinical Sciences, University of Edinburgh Medical School, Hugh Robson Building, George Square, Edinburgh, EH8 9XD, UK Genes and Development Group, Centre for Integrative Physiology, Clinical Sciences, University of Edinburgh Medical School, Hugh Robson Building, George Square, Edinburgh, EH8 9XD, UK Obstetrics and Gynaecology Section, Clinical Sciences, University of Edinburgh Medical School, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK Genes and Development Group, Centre for Integrative Physiology, Clinical Sciences, University of Edinburgh Medical School, Hugh Robson Building, George Square, Edinburgh, EH8 9XD, UK It has been shown previously that BALB/c strain embryos tend to contribute poorly to mouse aggregation chimaeras. In the present study we showed that BALB/c cells were not preferentially allocated to any extraembryonic lineages of mouse aggregation chimaeras, but their contribution decreased during the early postimplantation period and they were significantly depleted by E8.5. The development of BALB/c strain preimplantation embryos lagged behind embryos from some other strains and the contribution that BALB/c and other embryos made to chimaeras correlated with their developmental stage at E2.5. This relationship suggests that the poor contribution of BALB/c embryos to aggregation chimaeras is at least partly a consequence of generalised selection related to slow or delayed preimplantation development. The suitability of BALB/c embryos for maximising the ES cell contribution to mouse ES cell chimaeras is also discussed.http://bio.biologists.org/content/7/1/bio030189BALB/c mouse strainMouse embryoCell selectionChimaeraChimera |
| spellingShingle | Pin-Chi Tang Gillian E. MacKay Jean H. Flockhart Margaret A. Keighren Anna Kopakaki John D. West Selection against BALB/c strain cells in mouse chimaeras Biology Open BALB/c mouse strain Mouse embryo Cell selection Chimaera Chimera |
| title | Selection against BALB/c strain cells in mouse chimaeras |
| title_full | Selection against BALB/c strain cells in mouse chimaeras |
| title_fullStr | Selection against BALB/c strain cells in mouse chimaeras |
| title_full_unstemmed | Selection against BALB/c strain cells in mouse chimaeras |
| title_short | Selection against BALB/c strain cells in mouse chimaeras |
| title_sort | selection against balb c strain cells in mouse chimaeras |
| topic | BALB/c mouse strain Mouse embryo Cell selection Chimaera Chimera |
| url | http://bio.biologists.org/content/7/1/bio030189 |
| work_keys_str_mv | AT pinchitang selectionagainstbalbcstraincellsinmousechimaeras AT gillianemackay selectionagainstbalbcstraincellsinmousechimaeras AT jeanhflockhart selectionagainstbalbcstraincellsinmousechimaeras AT margaretakeighren selectionagainstbalbcstraincellsinmousechimaeras AT annakopakaki selectionagainstbalbcstraincellsinmousechimaeras AT johndwest selectionagainstbalbcstraincellsinmousechimaeras |