Using the Intrinsic Fluorescence of DNA to Characterize Aptamer Binding
The reliable, readily accessible and label-free measurement of aptamer binding remains a challenge in the field. Recent reports have shown large changes in the intrinsic fluorescence of DNA upon the formation of G-quadruplex and i-motif structures. In this work, we examined whether DNA intrinsic flu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/22/7809 |
| _version_ | 1797464452190175232 |
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| author | Chang Lu Anand Lopez Jinkai Zheng Juewen Liu |
| author_facet | Chang Lu Anand Lopez Jinkai Zheng Juewen Liu |
| author_sort | Chang Lu |
| collection | DOAJ |
| description | The reliable, readily accessible and label-free measurement of aptamer binding remains a challenge in the field. Recent reports have shown large changes in the intrinsic fluorescence of DNA upon the formation of G-quadruplex and i-motif structures. In this work, we examined whether DNA intrinsic fluorescence can be used for studying aptamer binding. First, DNA hybridization resulted in a drop in the fluorescence, which was observed for A30/T30 and a 24-mer random DNA sequence. Next, a series of DNA aptamers were studied. Cortisol and Hg<sup>2+</sup> induced fluorescence increases for their respective aptamers. For the cortisol aptamer, the length of the terminal stem needs to be short to produce a fluorescence change. However, caffeine and adenosine failed to produce a fluorescence change, regardless of the stem length. Overall, using the intrinsic fluorescence of DNA may be a reliable and accessible method to study a limited number of aptamers that can produce fluorescence changes. |
| first_indexed | 2024-03-09T18:08:20Z |
| format | Article |
| id | doaj.art-d550ee17c4e5451ba9ae266cdcbdcf73 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:08:20Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d550ee17c4e5451ba9ae266cdcbdcf732023-11-24T09:21:31ZengMDPI AGMolecules1420-30492022-11-012722780910.3390/molecules27227809Using the Intrinsic Fluorescence of DNA to Characterize Aptamer BindingChang Lu0Anand Lopez1Jinkai Zheng2Juewen Liu3Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaDepartment of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, CanadaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaDepartment of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, CanadaThe reliable, readily accessible and label-free measurement of aptamer binding remains a challenge in the field. Recent reports have shown large changes in the intrinsic fluorescence of DNA upon the formation of G-quadruplex and i-motif structures. In this work, we examined whether DNA intrinsic fluorescence can be used for studying aptamer binding. First, DNA hybridization resulted in a drop in the fluorescence, which was observed for A30/T30 and a 24-mer random DNA sequence. Next, a series of DNA aptamers were studied. Cortisol and Hg<sup>2+</sup> induced fluorescence increases for their respective aptamers. For the cortisol aptamer, the length of the terminal stem needs to be short to produce a fluorescence change. However, caffeine and adenosine failed to produce a fluorescence change, regardless of the stem length. Overall, using the intrinsic fluorescence of DNA may be a reliable and accessible method to study a limited number of aptamers that can produce fluorescence changes.https://www.mdpi.com/1420-3049/27/22/7809intrinsic fluorescenceDNAaptamerbinding |
| spellingShingle | Chang Lu Anand Lopez Jinkai Zheng Juewen Liu Using the Intrinsic Fluorescence of DNA to Characterize Aptamer Binding Molecules intrinsic fluorescence DNA aptamer binding |
| title | Using the Intrinsic Fluorescence of DNA to Characterize Aptamer Binding |
| title_full | Using the Intrinsic Fluorescence of DNA to Characterize Aptamer Binding |
| title_fullStr | Using the Intrinsic Fluorescence of DNA to Characterize Aptamer Binding |
| title_full_unstemmed | Using the Intrinsic Fluorescence of DNA to Characterize Aptamer Binding |
| title_short | Using the Intrinsic Fluorescence of DNA to Characterize Aptamer Binding |
| title_sort | using the intrinsic fluorescence of dna to characterize aptamer binding |
| topic | intrinsic fluorescence DNA aptamer binding |
| url | https://www.mdpi.com/1420-3049/27/22/7809 |
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