Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand
Zanthoxylum limonella (Dennst.) Alston. (Rutaceae) or Ma-khwaen is one of the medicinal plants in Thai traditional medicine. To investigate the pharmacognostic specifications and chemical constituents of Z. limonella fruits and seeds. Fruits and seeds of Z. limonella were collected from 15 sources t...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
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| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2016;volume=7;issue=4;spage=134;epage=138;aulast=Charoensup |
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| author | Rawiwan Charoensup Thidarat Duangyod Pravaree Phuneerub Chatubhong Singharachai |
| author_facet | Rawiwan Charoensup Thidarat Duangyod Pravaree Phuneerub Chatubhong Singharachai |
| author_sort | Rawiwan Charoensup |
| collection | DOAJ |
| description | Zanthoxylum limonella (Dennst.) Alston. (Rutaceae) or Ma-khwaen is one of the medicinal plants in Thai traditional medicine. To investigate the pharmacognostic specifications and chemical constituents of Z. limonella fruits and seeds. Fruits and seeds of Z. limonella were collected from 15 sources throughout Thailand; then examined the pharmacognostic specification following WHO guideline of quality control method for medicinal plant materials. Microscopic determination of Z. limonella powders demonstrated fragment of mesocarp, fragment of brown vitta, oil glands, fragment of endocarp, and endosperm containing oil globule, trichome and pale brown stone cells. Stomatal index and pellucid dots in mm 2 were 19.87 and 4.2 respectively. Physico-chemical parameters unveiled that loss on drying, water content, total ash, and acid-insoluble ash should be not >17.90%, 9.18%, 4.50%, and 0.60% of dried weight respectively; while ethanol, water, and hexane extractive values and volatile oil content should be not <2.24%, 2.27%, 1.57% and 9.63% of dried weight respectively. R f values of thin-layer chromatographic fingerprint of Z. limonella fruits and seeds ethanolic extract were 0.38, 0.45, 0.90, and 0.97 detected ultraviolet (UV) light 254 nm, 0.30, 0.44, 0.67, and 0.77 detected UV light 366 nm, and 0.24, 0.73, 0.78, and 0.93 detected 10% sulfuric acid. There are three main chemical compounds in Z. limonella oil including limonene (43.63%), (+)-sabinene (16.72%), and terpinen-4-ol (10.95%). The result gained from pharmacognostic specifications and chemical fingerprints could be used as standardization data of Z. limonella fruits and seeds to apply or provide for guarantee of quality. |
| first_indexed | 2024-12-11T19:01:25Z |
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| id | doaj.art-d552847172964b86850e99a37ae29c1f |
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| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-12-11T19:01:25Z |
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| publisher | Wolters Kluwer Medknow Publications |
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| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-d552847172964b86850e99a37ae29c1f2022-12-22T00:54:01ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942016-01-017413413810.4103/2231-4040.191421Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in ThailandRawiwan CharoensupThidarat DuangyodPravaree PhuneerubChatubhong SingharachaiZanthoxylum limonella (Dennst.) Alston. (Rutaceae) or Ma-khwaen is one of the medicinal plants in Thai traditional medicine. To investigate the pharmacognostic specifications and chemical constituents of Z. limonella fruits and seeds. Fruits and seeds of Z. limonella were collected from 15 sources throughout Thailand; then examined the pharmacognostic specification following WHO guideline of quality control method for medicinal plant materials. Microscopic determination of Z. limonella powders demonstrated fragment of mesocarp, fragment of brown vitta, oil glands, fragment of endocarp, and endosperm containing oil globule, trichome and pale brown stone cells. Stomatal index and pellucid dots in mm 2 were 19.87 and 4.2 respectively. Physico-chemical parameters unveiled that loss on drying, water content, total ash, and acid-insoluble ash should be not >17.90%, 9.18%, 4.50%, and 0.60% of dried weight respectively; while ethanol, water, and hexane extractive values and volatile oil content should be not <2.24%, 2.27%, 1.57% and 9.63% of dried weight respectively. R f values of thin-layer chromatographic fingerprint of Z. limonella fruits and seeds ethanolic extract were 0.38, 0.45, 0.90, and 0.97 detected ultraviolet (UV) light 254 nm, 0.30, 0.44, 0.67, and 0.77 detected UV light 366 nm, and 0.24, 0.73, 0.78, and 0.93 detected 10% sulfuric acid. There are three main chemical compounds in Z. limonella oil including limonene (43.63%), (+)-sabinene (16.72%), and terpinen-4-ol (10.95%). The result gained from pharmacognostic specifications and chemical fingerprints could be used as standardization data of Z. limonella fruits and seeds to apply or provide for guarantee of quality.http://www.japtr.org/article.asp?issn=2231-4040;year=2016;volume=7;issue=4;spage=134;epage=138;aulast=CharoensupGas chromatography/mass spectrometrypharmacognostic specificationphysico-chemical parameterthin-layer chromatographic fingerprintZanthoxylum limonella |
| spellingShingle | Rawiwan Charoensup Thidarat Duangyod Pravaree Phuneerub Chatubhong Singharachai Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand Journal of Advanced Pharmaceutical Technology & Research Gas chromatography/mass spectrometry pharmacognostic specification physico-chemical parameter thin-layer chromatographic fingerprint Zanthoxylum limonella |
| title | Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand |
| title_full | Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand |
| title_fullStr | Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand |
| title_full_unstemmed | Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand |
| title_short | Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand |
| title_sort | pharmacognostic specification of zanthoxylum limonella dennst alston fruits and seeds in thailand |
| topic | Gas chromatography/mass spectrometry pharmacognostic specification physico-chemical parameter thin-layer chromatographic fingerprint Zanthoxylum limonella |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2016;volume=7;issue=4;spage=134;epage=138;aulast=Charoensup |
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