Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesis
Endothelin-1 (ET-1) induces contraction, proliferation, and collagen synthesis of activated hepatic stellate cells and is a potent mediator of portal hypertension. Endothelin-converting enzyme-1 (ECE-1) generates ET-1 from the inactive precursor big-endothelin-1. The cellular distribution and activi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-05-01
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| Series: | Animal Cells and Systems |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19768354.2019.1595141 |
| _version_ | 1819082648618795008 |
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| author | Tae-Jun Cho Hyo-Jung Kim Jaejin Cho |
| author_facet | Tae-Jun Cho Hyo-Jung Kim Jaejin Cho |
| author_sort | Tae-Jun Cho |
| collection | DOAJ |
| description | Endothelin-1 (ET-1) induces contraction, proliferation, and collagen synthesis of activated hepatic stellate cells and is a potent mediator of portal hypertension. Endothelin-converting enzyme-1 (ECE-1) generates ET-1 from the inactive precursor big-endothelin-1. The cellular distribution and activity of ECE-1 in the liver is unknown. Hepatic fibrogenesis was induced in rats by CCl4 administration and secondary biliary cirrhosis after 6 weeks of complete bile duct occlusion (BDO). The tissue ET-1 and ET receptor protein levels were quantified, the ECE-1 isoform mRNAs were measured by RNase protection assay and ECE-1 activity was analyzed. ECE-1a and -b mRNA were upregulated in biliary cirrhosis and in CCl4-injured livers, whereas ECE-1c mRNA remained unchanged. ECE-1 activity was increased after BDO and peaked at 12 h after acute CCl4-intoxication. Tissue levels of ET-1, ETA- and ETB receptors were elevated 7-, 5-, and 4.6-fold in cirrhotic rats, respectively. ECE-1 activity increased following BDO and acute CCl4-intoxication. In conclusion, ECE-1a and -b RNAs are upregulated in fibrogenesis, indicating that these isoforms play a central role in ET-1 generation during fibrogenesis and portal hypertension. |
| first_indexed | 2024-12-21T20:20:01Z |
| format | Article |
| id | doaj.art-d552e579b9404b6486b22e3fe673e89f |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-12-21T20:20:01Z |
| publishDate | 2019-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-d552e579b9404b6486b22e3fe673e89f2022-12-21T18:51:30ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852019-05-0123317017510.1080/19768354.2019.15951411595141Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesisTae-Jun Cho0Hyo-Jung Kim1Jaejin Cho2School of Dentistry, Seoul National University South KoreaSeoul National UniversitySchool of Dentistry, Seoul National University South KoreaEndothelin-1 (ET-1) induces contraction, proliferation, and collagen synthesis of activated hepatic stellate cells and is a potent mediator of portal hypertension. Endothelin-converting enzyme-1 (ECE-1) generates ET-1 from the inactive precursor big-endothelin-1. The cellular distribution and activity of ECE-1 in the liver is unknown. Hepatic fibrogenesis was induced in rats by CCl4 administration and secondary biliary cirrhosis after 6 weeks of complete bile duct occlusion (BDO). The tissue ET-1 and ET receptor protein levels were quantified, the ECE-1 isoform mRNAs were measured by RNase protection assay and ECE-1 activity was analyzed. ECE-1a and -b mRNA were upregulated in biliary cirrhosis and in CCl4-injured livers, whereas ECE-1c mRNA remained unchanged. ECE-1 activity was increased after BDO and peaked at 12 h after acute CCl4-intoxication. Tissue levels of ET-1, ETA- and ETB receptors were elevated 7-, 5-, and 4.6-fold in cirrhotic rats, respectively. ECE-1 activity increased following BDO and acute CCl4-intoxication. In conclusion, ECE-1a and -b RNAs are upregulated in fibrogenesis, indicating that these isoforms play a central role in ET-1 generation during fibrogenesis and portal hypertension.http://dx.doi.org/10.1080/19768354.2019.1595141Endothelin-converting enzyme-1endothelin-1hepatic fibrogenesisendothelin receptor |
| spellingShingle | Tae-Jun Cho Hyo-Jung Kim Jaejin Cho Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesis Animal Cells and Systems Endothelin-converting enzyme-1 endothelin-1 hepatic fibrogenesis endothelin receptor |
| title | Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesis |
| title_full | Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesis |
| title_fullStr | Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesis |
| title_full_unstemmed | Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesis |
| title_short | Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesis |
| title_sort | endothelin converting enzyme 1 expression in acute and chronic liver injury in fibrogenesis |
| topic | Endothelin-converting enzyme-1 endothelin-1 hepatic fibrogenesis endothelin receptor |
| url | http://dx.doi.org/10.1080/19768354.2019.1595141 |
| work_keys_str_mv | AT taejuncho endothelinconvertingenzyme1expressioninacuteandchronicliverinjuryinfibrogenesis AT hyojungkim endothelinconvertingenzyme1expressioninacuteandchronicliverinjuryinfibrogenesis AT jaejincho endothelinconvertingenzyme1expressioninacuteandchronicliverinjuryinfibrogenesis |