The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase
The effect of three types of polysaccharides (agar-agar, carrageenan, hydroxypropyl methylcellulose) on the activity and stability during storage at given temperature conditions of the enzyme preparation L-phenylalanine ammonia-lyase was studied. It was found that the most suitable storage temperatu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019367556 |
| _version_ | 1830284183846518784 |
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| author | Olga Babich Lyubov Dyshlyuk Svetlana Noskova Alexander Prosekov Svetlana Ivanova Valery Pavsky |
| author_facet | Olga Babich Lyubov Dyshlyuk Svetlana Noskova Alexander Prosekov Svetlana Ivanova Valery Pavsky |
| author_sort | Olga Babich |
| collection | DOAJ |
| description | The effect of three types of polysaccharides (agar-agar, carrageenan, hydroxypropyl methylcellulose) on the activity and stability during storage at given temperature conditions of the enzyme preparation L-phenylalanine ammonia-lyase was studied. It was found that the most suitable storage temperature for encapsulated L-phenylalanine-ammonia-lyase is room temperature up to 25 °C for all samples of capsules from plant polysaccharides. Samples of capsules with agar-agar and hydroxypropyl methylcellulose under different temperature conditions inhibited the decrease in enzyme activity, which in other samples of capsules reached 90% in 6 months of storage. In samples of capsules with carrageenan at temperatures of 4 °C and 30 °C, there was a significant decrease in the activity of the enzyme preparation. Selection of capsule samples from plant polysaccharides suitable for L-phenylalanine-ammonia-lyase replacement therapy is done after studying the mechanisms of capsule destruction under conditions close to the conditions of the gastrointestinal tract, to which the next stage of our research will be devoted. |
| first_indexed | 2024-12-19T03:14:43Z |
| format | Article |
| id | doaj.art-d554d9d8f6174221a42df0050d661530 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-19T03:14:43Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-d554d9d8f6174221a42df0050d6615302022-12-21T20:37:55ZengElsevierHeliyon2405-84402020-01-0161e03096The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyaseOlga Babich0Lyubov Dyshlyuk1Svetlana Noskova2Alexander Prosekov3Svetlana Ivanova4Valery Pavsky5Laboratory of Biocatalysis, Kemerovo State University, Krasnaya Street 6, Kemerovo, 650043, Russia; Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, Kaliningrad, 236016, RussiaResearch Institute of Biotechnology, Kemerovo State University, Krasnaya Street 6, Kemerovo, 650043, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, Kaliningrad, 236016, RussiaLaboratory of Biocatalysis, Kemerovo State University, Krasnaya Street 6, Kemerovo, 650043, RussiaResearch Institute of Biotechnology, Kemerovo State University, Krasnaya Street 6, Kemerovo, 650043, Russia; Department of General Mathematics and Informatics, Kemerovo State University, Krasnaya Street 6, Kemerovo 650043, Russia; Corresponding author.Research Institute of Biotechnology, Kemerovo State University, Krasnaya Street 6, Kemerovo, 650043, Russia; Department of General Mathematics and Informatics, Kemerovo State University, Krasnaya Street 6, Kemerovo 650043, RussiaThe effect of three types of polysaccharides (agar-agar, carrageenan, hydroxypropyl methylcellulose) on the activity and stability during storage at given temperature conditions of the enzyme preparation L-phenylalanine ammonia-lyase was studied. It was found that the most suitable storage temperature for encapsulated L-phenylalanine-ammonia-lyase is room temperature up to 25 °C for all samples of capsules from plant polysaccharides. Samples of capsules with agar-agar and hydroxypropyl methylcellulose under different temperature conditions inhibited the decrease in enzyme activity, which in other samples of capsules reached 90% in 6 months of storage. In samples of capsules with carrageenan at temperatures of 4 °C and 30 °C, there was a significant decrease in the activity of the enzyme preparation. Selection of capsule samples from plant polysaccharides suitable for L-phenylalanine-ammonia-lyase replacement therapy is done after studying the mechanisms of capsule destruction under conditions close to the conditions of the gastrointestinal tract, to which the next stage of our research will be devoted.http://www.sciencedirect.com/science/article/pii/S2405844019367556Pharmaceutical chemistryBiotechnologyPharmaceutical scienceCapsulesNatural polysaccharidesEnzyme preparation |
| spellingShingle | Olga Babich Lyubov Dyshlyuk Svetlana Noskova Alexander Prosekov Svetlana Ivanova Valery Pavsky The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase Heliyon Pharmaceutical chemistry Biotechnology Pharmaceutical science Capsules Natural polysaccharides Enzyme preparation |
| title | The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase |
| title_full | The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase |
| title_fullStr | The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase |
| title_full_unstemmed | The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase |
| title_short | The effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of L-phenylalanine-ammonia-lyase |
| title_sort | effectiveness of plant hydrocolloids at maintaining the quality characteristics of the encapsulated form of l phenylalanine ammonia lyase |
| topic | Pharmaceutical chemistry Biotechnology Pharmaceutical science Capsules Natural polysaccharides Enzyme preparation |
| url | http://www.sciencedirect.com/science/article/pii/S2405844019367556 |
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