Genetic Diversity of <i>Colletotrichum</i> spp. Causing Grape Anthracnose in Zhejiang, China
Anthracnose is a fungal disease that seriously threatens grape production and quality. Multiple <i>Colletotrichum</i> species are detected in anthracnose grapes in vineyards. In this study, diseased grapes were collected in four counties in Zhejiang, and 43 <i>Colletotrichum</i&...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/4/952 |
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| author | Boyang Ye Jingqun Zhang Xiangyang Chen Wenfei Xiao Jianyan Wu Hong Yu Chuanqing Zhang |
| author_facet | Boyang Ye Jingqun Zhang Xiangyang Chen Wenfei Xiao Jianyan Wu Hong Yu Chuanqing Zhang |
| author_sort | Boyang Ye |
| collection | DOAJ |
| description | Anthracnose is a fungal disease that seriously threatens grape production and quality. Multiple <i>Colletotrichum</i> species are detected in anthracnose grapes in vineyards. In this study, diseased grapes were collected in four counties in Zhejiang, and 43 <i>Colletotrichum</i> isolates were obtained. Multi-genes (ITS, <i>TUB2</i>, <i>ACT</i>, <i>CHS-I</i>, and <i>GAPDH</i>) and morphological characteristic analyses showed that <i>C. fructicola</i> (40 isolates, 93%), <i>C. aenigma</i> (two isolates, 4.7%), and <i>C. pseudoacutatum</i> (one isolate, 2.3%) were the cause of grape anthracnose in Zhejiang. Among the three <i>Colletotrichum</i> species, <i>C. fructicola</i> was the prevalent and dominant species in all sampled counties; <i>C. pseudoacutatum</i> was first identified as the pathogen responsible for grape anthracnose. There were significant differences in the sporulation among the three <i>Colletotrichum</i> species, as well as in the spore germination. Pathogenicity testing showed that all species can infect grapes, resulting in anthracnose. On the other hand, the virulence of species was varied and may be associated with their spore germination. This is the first study to characterize the <i>Colletotrichum</i> species causing grape anthracnose in Zhejiang Province and reveal that <i>C. fructicola</i> is the dominant species. The determination of <i>Colletotrichum</i> species associated with grape anthracnose may contribute to the study of epidemiology and development of an efficient strategy for controlling anthracnose in the vineyards. |
| first_indexed | 2024-03-11T05:20:19Z |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T05:20:19Z |
| publishDate | 2023-03-01 |
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| series | Agronomy |
| spelling | doaj.art-d55a33ed4e5e4cda8742b4e3792b2a342023-11-17T17:55:26ZengMDPI AGAgronomy2073-43952023-03-0113495210.3390/agronomy13040952Genetic Diversity of <i>Colletotrichum</i> spp. Causing Grape Anthracnose in Zhejiang, ChinaBoyang Ye0Jingqun Zhang1Xiangyang Chen2Wenfei Xiao3Jianyan Wu4Hong Yu5Chuanqing Zhang6College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaResearch Institute for the Agriculture Science of Hangzhou, Hangzhou 310013, ChinaCollege of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaResearch Institute for the Agriculture Science of Hangzhou, Hangzhou 310013, ChinaCollege of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaAnthracnose is a fungal disease that seriously threatens grape production and quality. Multiple <i>Colletotrichum</i> species are detected in anthracnose grapes in vineyards. In this study, diseased grapes were collected in four counties in Zhejiang, and 43 <i>Colletotrichum</i> isolates were obtained. Multi-genes (ITS, <i>TUB2</i>, <i>ACT</i>, <i>CHS-I</i>, and <i>GAPDH</i>) and morphological characteristic analyses showed that <i>C. fructicola</i> (40 isolates, 93%), <i>C. aenigma</i> (two isolates, 4.7%), and <i>C. pseudoacutatum</i> (one isolate, 2.3%) were the cause of grape anthracnose in Zhejiang. Among the three <i>Colletotrichum</i> species, <i>C. fructicola</i> was the prevalent and dominant species in all sampled counties; <i>C. pseudoacutatum</i> was first identified as the pathogen responsible for grape anthracnose. There were significant differences in the sporulation among the three <i>Colletotrichum</i> species, as well as in the spore germination. Pathogenicity testing showed that all species can infect grapes, resulting in anthracnose. On the other hand, the virulence of species was varied and may be associated with their spore germination. This is the first study to characterize the <i>Colletotrichum</i> species causing grape anthracnose in Zhejiang Province and reveal that <i>C. fructicola</i> is the dominant species. The determination of <i>Colletotrichum</i> species associated with grape anthracnose may contribute to the study of epidemiology and development of an efficient strategy for controlling anthracnose in the vineyards.https://www.mdpi.com/2073-4395/13/4/952grape anthracnose<i>Colletotrichum</i> speciesmulti-gene analysis<i>C. fructicola</i><i>C. pseudoacutatum</i>spore germination |
| spellingShingle | Boyang Ye Jingqun Zhang Xiangyang Chen Wenfei Xiao Jianyan Wu Hong Yu Chuanqing Zhang Genetic Diversity of <i>Colletotrichum</i> spp. Causing Grape Anthracnose in Zhejiang, China Agronomy grape anthracnose <i>Colletotrichum</i> species multi-gene analysis <i>C. fructicola</i> <i>C. pseudoacutatum</i> spore germination |
| title | Genetic Diversity of <i>Colletotrichum</i> spp. Causing Grape Anthracnose in Zhejiang, China |
| title_full | Genetic Diversity of <i>Colletotrichum</i> spp. Causing Grape Anthracnose in Zhejiang, China |
| title_fullStr | Genetic Diversity of <i>Colletotrichum</i> spp. Causing Grape Anthracnose in Zhejiang, China |
| title_full_unstemmed | Genetic Diversity of <i>Colletotrichum</i> spp. Causing Grape Anthracnose in Zhejiang, China |
| title_short | Genetic Diversity of <i>Colletotrichum</i> spp. Causing Grape Anthracnose in Zhejiang, China |
| title_sort | genetic diversity of i colletotrichum i spp causing grape anthracnose in zhejiang china |
| topic | grape anthracnose <i>Colletotrichum</i> species multi-gene analysis <i>C. fructicola</i> <i>C. pseudoacutatum</i> spore germination |
| url | https://www.mdpi.com/2073-4395/13/4/952 |
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