Ultrasound-assisted extraction, optimization, isolation, and antioxidant activity analysis of flavonoids from Astragalus membranaceus stems and leaves
Astragalus membranaceus is a medicinal and edible species in China, with a variety of biological activities. This study evaluated the reuse potential of A. membranaceus waste as a source of food antioxidants. Antioxidant and antifungal activities of flavonoids, polysaccharides, and saponins from A....
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417722002863 |
| _version_ | 1811189377612644352 |
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| author | Liyan Cui Zhennan Ma Defu Wang Yanbing Niu |
| author_facet | Liyan Cui Zhennan Ma Defu Wang Yanbing Niu |
| author_sort | Liyan Cui |
| collection | DOAJ |
| description | Astragalus membranaceus is a medicinal and edible species in China, with a variety of biological activities. This study evaluated the reuse potential of A. membranaceus waste as a source of food antioxidants. Antioxidant and antifungal activities of flavonoids, polysaccharides, and saponins from A. membranaceus stems and leaves were evaluated. Results showed that inhibition rate of flavonoids on six tested fungi reaches 100 % at a concentration of 5 mg/mL, and the antioxidant test demonstrated satisfactory antioxidant activity. On this basis, an extremely economical ultrasonic-assisted extraction of flavonoids from A. membranaceus stems and leaves was developed and optimized via response surface methodology (RSM). Optimized conditions included an extraction time of 35 min, ethanol concentration of 75 %, liquid–solid ratio of 40 mL/g, and extraction temperature of 58 °C, in which the extraction yield of flavonoids was 22.0270 ± 2.5739 mg/g. The total flavonoids were separated and purified using activity-guided isolation technology, and frac. ccd with strong antioxidant activity were analyzed via HPLC-MS/MS. Results showed that main components are isoquercitrin and astragalin. This study can provide a potential innovative application for the development of natural food antioxidants from A. membranaceus waste. |
| first_indexed | 2024-04-11T14:34:39Z |
| format | Article |
| id | doaj.art-d561c4a449fc40c1a79000fe4dc84f0c |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-04-11T14:34:39Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-d561c4a449fc40c1a79000fe4dc84f0c2022-12-22T04:18:24ZengElsevierUltrasonics Sonochemistry1350-41772022-11-0190106190Ultrasound-assisted extraction, optimization, isolation, and antioxidant activity analysis of flavonoids from Astragalus membranaceus stems and leavesLiyan Cui0Zhennan Ma1Defu Wang2Yanbing Niu3College of Grassland Science, Shanxi Agricultural University, Taigu 030801, Shanxi, PR ChinaCollege of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi, PR ChinaCollege of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi, PR ChinaCollege of Grassland Science, Shanxi Agricultural University, Taigu 030801, Shanxi, PR China; College of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi, PR China; Corresponding author at: College of Grassland Science, Shanxi Agricultural University, Taigu 030801, Shanxi, PR China.Astragalus membranaceus is a medicinal and edible species in China, with a variety of biological activities. This study evaluated the reuse potential of A. membranaceus waste as a source of food antioxidants. Antioxidant and antifungal activities of flavonoids, polysaccharides, and saponins from A. membranaceus stems and leaves were evaluated. Results showed that inhibition rate of flavonoids on six tested fungi reaches 100 % at a concentration of 5 mg/mL, and the antioxidant test demonstrated satisfactory antioxidant activity. On this basis, an extremely economical ultrasonic-assisted extraction of flavonoids from A. membranaceus stems and leaves was developed and optimized via response surface methodology (RSM). Optimized conditions included an extraction time of 35 min, ethanol concentration of 75 %, liquid–solid ratio of 40 mL/g, and extraction temperature of 58 °C, in which the extraction yield of flavonoids was 22.0270 ± 2.5739 mg/g. The total flavonoids were separated and purified using activity-guided isolation technology, and frac. ccd with strong antioxidant activity were analyzed via HPLC-MS/MS. Results showed that main components are isoquercitrin and astragalin. This study can provide a potential innovative application for the development of natural food antioxidants from A. membranaceus waste.http://www.sciencedirect.com/science/article/pii/S1350417722002863Astragalus membranaceus stems and leavesFlavonoidsAnti-fungal and antioxidant activityUltrasound-assisted extraction and optimizationActivity-guided isolationMolecular docking |
| spellingShingle | Liyan Cui Zhennan Ma Defu Wang Yanbing Niu Ultrasound-assisted extraction, optimization, isolation, and antioxidant activity analysis of flavonoids from Astragalus membranaceus stems and leaves Ultrasonics Sonochemistry Astragalus membranaceus stems and leaves Flavonoids Anti-fungal and antioxidant activity Ultrasound-assisted extraction and optimization Activity-guided isolation Molecular docking |
| title | Ultrasound-assisted extraction, optimization, isolation, and antioxidant activity analysis of flavonoids from Astragalus membranaceus stems and leaves |
| title_full | Ultrasound-assisted extraction, optimization, isolation, and antioxidant activity analysis of flavonoids from Astragalus membranaceus stems and leaves |
| title_fullStr | Ultrasound-assisted extraction, optimization, isolation, and antioxidant activity analysis of flavonoids from Astragalus membranaceus stems and leaves |
| title_full_unstemmed | Ultrasound-assisted extraction, optimization, isolation, and antioxidant activity analysis of flavonoids from Astragalus membranaceus stems and leaves |
| title_short | Ultrasound-assisted extraction, optimization, isolation, and antioxidant activity analysis of flavonoids from Astragalus membranaceus stems and leaves |
| title_sort | ultrasound assisted extraction optimization isolation and antioxidant activity analysis of flavonoids from astragalus membranaceus stems and leaves |
| topic | Astragalus membranaceus stems and leaves Flavonoids Anti-fungal and antioxidant activity Ultrasound-assisted extraction and optimization Activity-guided isolation Molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S1350417722002863 |
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