Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 Cells
Benzo[a]pyrene (B[a]P) is a potentially hepatotoxic group-1 carcinogen taken up by the body through ingestion of daily foods. B[a]P is widely known to cause DNA and protein damages, which are closely related to cell transformation. Accordingly, studies on natural bioactive compounds that attenuate s...
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MDPI AG
2021-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/8/1314 |
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| author | Seung-Cheol Lee Seung-Cheol Jee Min Kim Soee Kim Min Kyoung Shin Yunkyung Kim Jung-Suk Sung |
| author_facet | Seung-Cheol Lee Seung-Cheol Jee Min Kim Soee Kim Min Kyoung Shin Yunkyung Kim Jung-Suk Sung |
| author_sort | Seung-Cheol Lee |
| collection | DOAJ |
| description | Benzo[a]pyrene (B[a]P) is a potentially hepatotoxic group-1 carcinogen taken up by the body through ingestion of daily foods. B[a]P is widely known to cause DNA and protein damages, which are closely related to cell transformation. Accordingly, studies on natural bioactive compounds that attenuate such chemical-induced toxicities have significant impacts on public health. This study aimed to uncover the mechanism of curcumin, the major curcuminoid in turmeric (<i>Curcuma longa</i>), in modulating the lipid accumulation and oxidative stress mediated by B[a]P cytotoxicity in HepG2 cells. Curcumin treatment reduced the B[a]P-induced lipid accumulation and reactive oxygen spicies (ROS) upregulation and recovered the cell viability. Cytochrome P450 family 1 subfamily A polypeptide 1 (CYP1A1) and Cytochrome P450 subfamily B polypeptide 1 (CYP1B1) downregulation resulting from decreased aryl hydrocarbon receptor (AhR) translocation into nuclei attenuated the effects of B[a]P-induced lipid accumulation and repressed cell viability, respectively. Moreover, the curcumin-induced reduction in ROS generation decreased the nuclear translocation of Nuclear factor erythroid-2-related factor 2 (Nrf2) and the expression of phase-II detoxifying enzymes. These results indicate that curcumin suppresses B[a]P-induced lipid accumulation and ROS generation which can potentially induce nonalcoholic fatty liver disease (NAFLD) and can shed a light on the detoxifying effect of curcumin. |
| first_indexed | 2024-03-10T09:03:44Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T09:03:44Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-d565b895ab1d4991aa8a128bdfcb81c62023-11-22T06:37:20ZengMDPI AGAntioxidants2076-39212021-08-01108131410.3390/antiox10081314Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 CellsSeung-Cheol Lee0Seung-Cheol Jee1Min Kim2Soee Kim3Min Kyoung Shin4Yunkyung Kim5Jung-Suk Sung6Department of Life Science, Dongguk University-Seoul, Goyang 10326, KoreaDepartment of Life Science, Dongguk University-Seoul, Goyang 10326, KoreaDepartment of Life Science, Dongguk University-Seoul, Goyang 10326, KoreaDepartment of Life Science, Dongguk University-Seoul, Goyang 10326, KoreaDepartment of Life Science, Dongguk University-Seoul, Goyang 10326, KoreaDepartment of Life Science, Dongguk University-Seoul, Goyang 10326, KoreaDepartment of Life Science, Dongguk University-Seoul, Goyang 10326, KoreaBenzo[a]pyrene (B[a]P) is a potentially hepatotoxic group-1 carcinogen taken up by the body through ingestion of daily foods. B[a]P is widely known to cause DNA and protein damages, which are closely related to cell transformation. Accordingly, studies on natural bioactive compounds that attenuate such chemical-induced toxicities have significant impacts on public health. This study aimed to uncover the mechanism of curcumin, the major curcuminoid in turmeric (<i>Curcuma longa</i>), in modulating the lipid accumulation and oxidative stress mediated by B[a]P cytotoxicity in HepG2 cells. Curcumin treatment reduced the B[a]P-induced lipid accumulation and reactive oxygen spicies (ROS) upregulation and recovered the cell viability. Cytochrome P450 family 1 subfamily A polypeptide 1 (CYP1A1) and Cytochrome P450 subfamily B polypeptide 1 (CYP1B1) downregulation resulting from decreased aryl hydrocarbon receptor (AhR) translocation into nuclei attenuated the effects of B[a]P-induced lipid accumulation and repressed cell viability, respectively. Moreover, the curcumin-induced reduction in ROS generation decreased the nuclear translocation of Nuclear factor erythroid-2-related factor 2 (Nrf2) and the expression of phase-II detoxifying enzymes. These results indicate that curcumin suppresses B[a]P-induced lipid accumulation and ROS generation which can potentially induce nonalcoholic fatty liver disease (NAFLD) and can shed a light on the detoxifying effect of curcumin.https://www.mdpi.com/2076-3921/10/8/1314curcuminlipid accumulationB[a]P toxicityROSCYP1A1CYP1B1 |
| spellingShingle | Seung-Cheol Lee Seung-Cheol Jee Min Kim Soee Kim Min Kyoung Shin Yunkyung Kim Jung-Suk Sung Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 Cells Antioxidants curcumin lipid accumulation B[a]P toxicity ROS CYP1A1 CYP1B1 |
| title | Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 Cells |
| title_full | Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 Cells |
| title_fullStr | Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 Cells |
| title_full_unstemmed | Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 Cells |
| title_short | Curcumin Suppresses the Lipid Accumulation and Oxidative Stress Induced by Benzo[a]pyrene Toxicity in HepG2 Cells |
| title_sort | curcumin suppresses the lipid accumulation and oxidative stress induced by benzo a pyrene toxicity in hepg2 cells |
| topic | curcumin lipid accumulation B[a]P toxicity ROS CYP1A1 CYP1B1 |
| url | https://www.mdpi.com/2076-3921/10/8/1314 |
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