Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method
This study aimed to determine the resistance profile and the nature of multidrug resistance in Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E.coli) against several classes of antibiotics. Positive isolates of ESBL-producing E.coli were tested for antibiotic sensitivity using t...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2020-05-01
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| Series: | Buletin Peternakan |
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| Online Access: | https://jurnal.ugm.ac.id/buletinpeternakan/article/view/51347 |
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| author | Freshinta Jellia Wibisono Bambang Sumiarto Tri Untari Mustofa Helmi Effendi Dian Ayu Permatasari Adiana Mutamsari Witaningrum |
| author_facet | Freshinta Jellia Wibisono Bambang Sumiarto Tri Untari Mustofa Helmi Effendi Dian Ayu Permatasari Adiana Mutamsari Witaningrum |
| author_sort | Freshinta Jellia Wibisono |
| collection | DOAJ |
| description | This study aimed to determine the resistance profile and the nature of multidrug resistance in Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E.coli) against several classes of antibiotics. Positive isolates of ESBL-producing E.coli were tested for antibiotic sensitivity using the VITEK® 2 compact method which then analyzed automatically. The results showed an antibiotic resistance profile against ESBL-producing E.coli showed the highest level of antibiotics in beta lactam, amoxicillin, ampicillin, cefazolin, cefotaxime, and ceftriaxone at 100%. Subsequent results found a relatively high level of resistance in the antibiotics aztreonam (86.36%), trimethoprim/sulfamethoxazole (77.27%), gentamicin (72.73%), and ciprofloxacin (68.18%). Antibiotics from carbapenem groups such as ertapenem and memenem, and antibiotics from the aminoglycosides (amicasin) and tigecycline groups of tetracycline still showed a high sensitivity level of 100%. The most common resistance patterns found in ESBL-producing E.coli isolates are AM/AMP/KZ/CTX/CRO/ATM/GM/CIP as much as 22.73%, and AM/AMP/KZ/CTX/CRO/ATM/GM/CIP/SXT patterns of 18.2%. The results of multi-class antibiotic resistance showed that 86.36% had multidrug resistance. The highest multidrug resistance pattern in ESBL-producing E.coli occurred with a BL/AG/Q/SP pattern of 50%. Other patterns of multidrug resistance in ESBL-producing E.coli that can be found in this study are, the BL/AG/Q/SP pattern is 18.20%, the BL/AG/Q/SP pattern is 13.64%, and the BL/AG/Q pattern is 4.55%. The high profile of resistance and the nature of multidrug resistance in ESBL-producing E.coli has the potential to spread these resistant genes, thus risking the use of antibiotics as a public health therapy and animal health, therefore further evaluation and control are needed. |
| first_indexed | 2024-12-12T07:11:45Z |
| format | Article |
| id | doaj.art-d5661de8ce864a7f9bfd94ec6701475d |
| institution | Directory Open Access Journal |
| issn | 0126-4400 2407-876X |
| language | English |
| last_indexed | 2024-12-12T07:11:45Z |
| publishDate | 2020-05-01 |
| publisher | Universitas Gadjah Mada |
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| series | Buletin Peternakan |
| spelling | doaj.art-d5661de8ce864a7f9bfd94ec6701475d2022-12-22T00:33:36ZengUniversitas Gadjah MadaBuletin Peternakan0126-44002407-876X2020-05-0144210.21059/buletinpeternak.v44i2.5134726888Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact MethodFreshinta Jellia Wibisono0Bambang Sumiarto1Tri Untari2Mustofa Helmi Effendi3Dian Ayu Permatasari4Adiana Mutamsari Witaningrum5Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, IndonesiaDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, IndonesiaDepartment of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, IndonesiaDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya, 60115, IndonesiaDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya, 60115, IndonesiaDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya, 60115, IndonesiaThis study aimed to determine the resistance profile and the nature of multidrug resistance in Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E.coli) against several classes of antibiotics. Positive isolates of ESBL-producing E.coli were tested for antibiotic sensitivity using the VITEK® 2 compact method which then analyzed automatically. The results showed an antibiotic resistance profile against ESBL-producing E.coli showed the highest level of antibiotics in beta lactam, amoxicillin, ampicillin, cefazolin, cefotaxime, and ceftriaxone at 100%. Subsequent results found a relatively high level of resistance in the antibiotics aztreonam (86.36%), trimethoprim/sulfamethoxazole (77.27%), gentamicin (72.73%), and ciprofloxacin (68.18%). Antibiotics from carbapenem groups such as ertapenem and memenem, and antibiotics from the aminoglycosides (amicasin) and tigecycline groups of tetracycline still showed a high sensitivity level of 100%. The most common resistance patterns found in ESBL-producing E.coli isolates are AM/AMP/KZ/CTX/CRO/ATM/GM/CIP as much as 22.73%, and AM/AMP/KZ/CTX/CRO/ATM/GM/CIP/SXT patterns of 18.2%. The results of multi-class antibiotic resistance showed that 86.36% had multidrug resistance. The highest multidrug resistance pattern in ESBL-producing E.coli occurred with a BL/AG/Q/SP pattern of 50%. Other patterns of multidrug resistance in ESBL-producing E.coli that can be found in this study are, the BL/AG/Q/SP pattern is 18.20%, the BL/AG/Q/SP pattern is 13.64%, and the BL/AG/Q pattern is 4.55%. The high profile of resistance and the nature of multidrug resistance in ESBL-producing E.coli has the potential to spread these resistant genes, thus risking the use of antibiotics as a public health therapy and animal health, therefore further evaluation and control are needed.https://jurnal.ugm.ac.id/buletinpeternakan/article/view/51347esbl-producing escherichia colimultidrug resistancevitek® 2 compact method |
| spellingShingle | Freshinta Jellia Wibisono Bambang Sumiarto Tri Untari Mustofa Helmi Effendi Dian Ayu Permatasari Adiana Mutamsari Witaningrum Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method Buletin Peternakan esbl-producing escherichia coli multidrug resistance vitek® 2 compact method |
| title | Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method |
| title_full | Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method |
| title_fullStr | Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method |
| title_full_unstemmed | Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method |
| title_short | Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method |
| title_sort | resistance profile of extended spectrum beta lactamase producing escherichia coli bacteria using vitek r 2 compact method |
| topic | esbl-producing escherichia coli multidrug resistance vitek® 2 compact method |
| url | https://jurnal.ugm.ac.id/buletinpeternakan/article/view/51347 |
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