Multiple Fingerprints and Spectrum-Effect Relationship of Polysaccharides from <i>Saposhnikoviae Radix</i>
PMP-HPLC, FT-IR, and HPSEC fingerprints of 10 batches of polysaccharides from <i>Saposhnikoviae Radix</i> with different production areas and harvest times have been prepared, and the chemometrics analysis was performed. The anti-allergic activity of 10 batches of <i>Saposhnikoviae...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/16/5278 |
| _version_ | 1797443107415916544 |
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| author | Mengqi Yu Guang Xu Ming Qin Yanling Li Yuying Guo Qun Ma |
| author_facet | Mengqi Yu Guang Xu Ming Qin Yanling Li Yuying Guo Qun Ma |
| author_sort | Mengqi Yu |
| collection | DOAJ |
| description | PMP-HPLC, FT-IR, and HPSEC fingerprints of 10 batches of polysaccharides from <i>Saposhnikoviae Radix</i> with different production areas and harvest times have been prepared, and the chemometrics analysis was performed. The anti-allergic activity of 10 batches of <i>Saposhnikoviae Radix</i> polysaccharide (SP) was evaluated, and the spectrum-effect relationship of the 10 batches of SP was analyzed by gray correlation degree with the chromatographic fingerprint as the independent variable. The results showed that the PMP-HPLC, HPSEC, and FT-IR fingerprints of 10 batches of SP had a high similarity. Two monosaccharides (rhamnose and galactose), the polysaccharide fragment Mn = 8.67 × 10<sup>6</sup>~9.56 × 10<sup>6</sup> Da, and the FT-IR absorption peak of 892 cm<sup>−1</sup> can be used as the quality control markers of SPs. All 10 batches of SP could significantly inhibit the release of β-HEX in RBL-231 cells, and the polysaccharides harvested from Inner Mongolia in the winter had the best anti-allergic activity. The spectrum-effect relationship model showed that the monosaccharide composition and molecular weight were related to the anti-allergic activity of the SPs. Multiple fingerprints combined with spectrum-effect relationship analysis can evaluate and control the quality of SPs from the aspects of overall quality and efficacy, which has more application value. |
| first_indexed | 2024-03-09T12:51:21Z |
| format | Article |
| id | doaj.art-d5678cf5c81c4eb5bb79176656e046ba |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T12:51:21Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d5678cf5c81c4eb5bb79176656e046ba2023-11-30T22:05:31ZengMDPI AGMolecules1420-30492022-08-012716527810.3390/molecules27165278Multiple Fingerprints and Spectrum-Effect Relationship of Polysaccharides from <i>Saposhnikoviae Radix</i>Mengqi Yu0Guang Xu1Ming Qin2Yanling Li3Yuying Guo4Qun Ma5Department of Pharmacy of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaDepartment of Pharmacy of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaDepartment of Pharmacy of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaDepartment of Pharmacy of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaDepartment of Pharmacy of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaDepartment of Pharmacy of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaPMP-HPLC, FT-IR, and HPSEC fingerprints of 10 batches of polysaccharides from <i>Saposhnikoviae Radix</i> with different production areas and harvest times have been prepared, and the chemometrics analysis was performed. The anti-allergic activity of 10 batches of <i>Saposhnikoviae Radix</i> polysaccharide (SP) was evaluated, and the spectrum-effect relationship of the 10 batches of SP was analyzed by gray correlation degree with the chromatographic fingerprint as the independent variable. The results showed that the PMP-HPLC, HPSEC, and FT-IR fingerprints of 10 batches of SP had a high similarity. Two monosaccharides (rhamnose and galactose), the polysaccharide fragment Mn = 8.67 × 10<sup>6</sup>~9.56 × 10<sup>6</sup> Da, and the FT-IR absorption peak of 892 cm<sup>−1</sup> can be used as the quality control markers of SPs. All 10 batches of SP could significantly inhibit the release of β-HEX in RBL-231 cells, and the polysaccharides harvested from Inner Mongolia in the winter had the best anti-allergic activity. The spectrum-effect relationship model showed that the monosaccharide composition and molecular weight were related to the anti-allergic activity of the SPs. Multiple fingerprints combined with spectrum-effect relationship analysis can evaluate and control the quality of SPs from the aspects of overall quality and efficacy, which has more application value.https://www.mdpi.com/1420-3049/27/16/5278<i>Saposhnikoviae Radix</i> polysaccharidemultiple fingerprintsquality controlanti-allergic activityspectrum-effect relationship |
| spellingShingle | Mengqi Yu Guang Xu Ming Qin Yanling Li Yuying Guo Qun Ma Multiple Fingerprints and Spectrum-Effect Relationship of Polysaccharides from <i>Saposhnikoviae Radix</i> Molecules <i>Saposhnikoviae Radix</i> polysaccharide multiple fingerprints quality control anti-allergic activity spectrum-effect relationship |
| title | Multiple Fingerprints and Spectrum-Effect Relationship of Polysaccharides from <i>Saposhnikoviae Radix</i> |
| title_full | Multiple Fingerprints and Spectrum-Effect Relationship of Polysaccharides from <i>Saposhnikoviae Radix</i> |
| title_fullStr | Multiple Fingerprints and Spectrum-Effect Relationship of Polysaccharides from <i>Saposhnikoviae Radix</i> |
| title_full_unstemmed | Multiple Fingerprints and Spectrum-Effect Relationship of Polysaccharides from <i>Saposhnikoviae Radix</i> |
| title_short | Multiple Fingerprints and Spectrum-Effect Relationship of Polysaccharides from <i>Saposhnikoviae Radix</i> |
| title_sort | multiple fingerprints and spectrum effect relationship of polysaccharides from i saposhnikoviae radix i |
| topic | <i>Saposhnikoviae Radix</i> polysaccharide multiple fingerprints quality control anti-allergic activity spectrum-effect relationship |
| url | https://www.mdpi.com/1420-3049/27/16/5278 |
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