Hydrogen Production by Immobilized <i>Rhodopseudomonas</i> sp. Cells in Calcium Alginate Beads
The present investigation concerns the potentiality of <i>Rhodopseudomonas</i> sp. cells to produce clean energy such as molecular hydrogen (H<sub>2</sub>). The abovementioned goal could be reached by improving the capability of purple non-sulfur bacteria to produce H<sub&...
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MDPI AG
2022-11-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/15/22/8355 |
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| author | Eleftherios Touloupakis Angeliki Chatziathanasiou Demetrios F. Ghanotakis Pietro Carlozzi Isabella Pecorini |
| author_facet | Eleftherios Touloupakis Angeliki Chatziathanasiou Demetrios F. Ghanotakis Pietro Carlozzi Isabella Pecorini |
| author_sort | Eleftherios Touloupakis |
| collection | DOAJ |
| description | The present investigation concerns the potentiality of <i>Rhodopseudomonas</i> sp. cells to produce clean energy such as molecular hydrogen (H<sub>2</sub>). The abovementioned goal could be reached by improving the capability of purple non-sulfur bacteria to produce H<sub>2</sub> via a photofermentative process through the enzyme nitrogenase. <i>Rhodopseudomonas</i> sp. cells were immobilized in calcium alginate gel beads and cultured in a cylindrical photobioreactor at a working volume of 0.22 L. The semi-continuous process, which lasted for 11 days, was interspersed with the washing of the beads with the aim of increasing the H<sub>2</sub> production rate. The maximum H<sub>2</sub> production rate reached 5.25 ± 0.93 mL/h with a total output of 505 mL. The productivity was 40.9 μL (of H<sub>2</sub>)/mg (of cells)/h or 10.2 mL (of H<sub>2</sub>)/L (of culture)/h with a light conversion efficiency of 1.20%. |
| first_indexed | 2024-03-09T18:22:52Z |
| format | Article |
| id | doaj.art-d5688fdee0d443c88455c88100e4c689 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-09T18:22:52Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-d5688fdee0d443c88455c88100e4c6892023-11-24T08:11:48ZengMDPI AGEnergies1996-10732022-11-011522835510.3390/en15228355Hydrogen Production by Immobilized <i>Rhodopseudomonas</i> sp. Cells in Calcium Alginate BeadsEleftherios Touloupakis0Angeliki Chatziathanasiou1Demetrios F. Ghanotakis2Pietro Carlozzi3Isabella Pecorini4Research Institute on Terrestrial Ecosystems, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyDepartment of Chemistry, University of Crete, Voutes Campus, 70013 Heraklion, GreeceDepartment of Chemistry, University of Crete, Voutes Campus, 70013 Heraklion, GreeceResearch Institute on Terrestrial Ecosystems, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyDESTEC—Department of Energy, Systems, Territory and Construction Engineering, University of Pisa, 56122 Pisa, ItalyThe present investigation concerns the potentiality of <i>Rhodopseudomonas</i> sp. cells to produce clean energy such as molecular hydrogen (H<sub>2</sub>). The abovementioned goal could be reached by improving the capability of purple non-sulfur bacteria to produce H<sub>2</sub> via a photofermentative process through the enzyme nitrogenase. <i>Rhodopseudomonas</i> sp. cells were immobilized in calcium alginate gel beads and cultured in a cylindrical photobioreactor at a working volume of 0.22 L. The semi-continuous process, which lasted for 11 days, was interspersed with the washing of the beads with the aim of increasing the H<sub>2</sub> production rate. The maximum H<sub>2</sub> production rate reached 5.25 ± 0.93 mL/h with a total output of 505 mL. The productivity was 40.9 μL (of H<sub>2</sub>)/mg (of cells)/h or 10.2 mL (of H<sub>2</sub>)/L (of culture)/h with a light conversion efficiency of 1.20%.https://www.mdpi.com/1996-1073/15/22/8355<i>Rhodopseudomonas</i> sp.photobioreactorcalcium alginatephotofermentationhydrogen production |
| spellingShingle | Eleftherios Touloupakis Angeliki Chatziathanasiou Demetrios F. Ghanotakis Pietro Carlozzi Isabella Pecorini Hydrogen Production by Immobilized <i>Rhodopseudomonas</i> sp. Cells in Calcium Alginate Beads Energies <i>Rhodopseudomonas</i> sp. photobioreactor calcium alginate photofermentation hydrogen production |
| title | Hydrogen Production by Immobilized <i>Rhodopseudomonas</i> sp. Cells in Calcium Alginate Beads |
| title_full | Hydrogen Production by Immobilized <i>Rhodopseudomonas</i> sp. Cells in Calcium Alginate Beads |
| title_fullStr | Hydrogen Production by Immobilized <i>Rhodopseudomonas</i> sp. Cells in Calcium Alginate Beads |
| title_full_unstemmed | Hydrogen Production by Immobilized <i>Rhodopseudomonas</i> sp. Cells in Calcium Alginate Beads |
| title_short | Hydrogen Production by Immobilized <i>Rhodopseudomonas</i> sp. Cells in Calcium Alginate Beads |
| title_sort | hydrogen production by immobilized i rhodopseudomonas i sp cells in calcium alginate beads |
| topic | <i>Rhodopseudomonas</i> sp. photobioreactor calcium alginate photofermentation hydrogen production |
| url | https://www.mdpi.com/1996-1073/15/22/8355 |
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