Gene expression of HSP90 and HSP70 in four silkworm hybrids (Bombyx mori L.) in response to severe thermal shock
Usually silkworm egg producers provide several silkworm hybrids with different qualities of productivity and viability traits. This study was conducted to compare the expression of HSP70 and HSP90 genes among different silkworm hybrids. In the fourth day of fifth larval instar of two Japanese matern...
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2017-03-01
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| Series: | Invertebrate Survival Journal |
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| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/23 |
| _version_ | 1818904851115933696 |
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| author | S F Mousavi S H Hosseini Moghaddam N Ghavi Hossein-Zadeh S Z Mirhosseini |
| author_facet | S F Mousavi S H Hosseini Moghaddam N Ghavi Hossein-Zadeh S Z Mirhosseini |
| author_sort | S F Mousavi |
| collection | DOAJ |
| description | Usually silkworm egg producers provide several silkworm hybrids with different qualities of productivity and viability traits. This study was conducted to compare the expression of HSP70 and HSP90 genes among different silkworm hybrids. In the fourth day of fifth larval instar of two Japanese maternal parents (103×104 and 107×110) and two Chinese maternal parents (110×107 and 104×103), silkworm larval fat body was sampled from heat shock (45 °C for 35 min) and non-heat shock groups. Sampling was done at 0, 2, 4 and 24 h after heat shock. Gene expression of HSP70 and HSP90 (target genes) were measured by qRT-PCR using RPL27a as reference gene. The prolonged heat shock (8 h at 39 °C) was utilized to examine the thermal tolerance of larvae in comparison with control group (24°C). The results showed both HSP70 and HSP90 have been up-regulated in the treated larvae. The hybrids with Japanese female parents (107×110 and 103×104) were more sensitive than two others; moreover in these hybrids, HSP90 and HSP70 were expressed significantly more than others after heat exposure. The maximum expression was occurred in the time zero, then a decreasing trend was observed over time. Comparison of larval mortality among four hybrids revealed that (103×104) and (110×107) hybrids had the highest and the lowest mortality rate. |
| first_indexed | 2024-12-19T21:14:00Z |
| format | Article |
| id | doaj.art-d5695b1f53fd4cb08e6f41a718532d7f |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-19T21:14:00Z |
| publishDate | 2017-03-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-d5695b1f53fd4cb08e6f41a718532d7f2022-12-21T20:05:26ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2017-03-01141Gene expression of HSP90 and HSP70 in four silkworm hybrids (Bombyx mori L.) in response to severe thermal shockS F Mousavi0S H Hosseini Moghaddam1N Ghavi Hossein-Zadeh2S Z Mirhosseini3Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDepartment of Animal Science and Department of Sericulture, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDepartment of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranDepartment of Animal Science and Department of Sericulture, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranUsually silkworm egg producers provide several silkworm hybrids with different qualities of productivity and viability traits. This study was conducted to compare the expression of HSP70 and HSP90 genes among different silkworm hybrids. In the fourth day of fifth larval instar of two Japanese maternal parents (103×104 and 107×110) and two Chinese maternal parents (110×107 and 104×103), silkworm larval fat body was sampled from heat shock (45 °C for 35 min) and non-heat shock groups. Sampling was done at 0, 2, 4 and 24 h after heat shock. Gene expression of HSP70 and HSP90 (target genes) were measured by qRT-PCR using RPL27a as reference gene. The prolonged heat shock (8 h at 39 °C) was utilized to examine the thermal tolerance of larvae in comparison with control group (24°C). The results showed both HSP70 and HSP90 have been up-regulated in the treated larvae. The hybrids with Japanese female parents (107×110 and 103×104) were more sensitive than two others; moreover in these hybrids, HSP90 and HSP70 were expressed significantly more than others after heat exposure. The maximum expression was occurred in the time zero, then a decreasing trend was observed over time. Comparison of larval mortality among four hybrids revealed that (103×104) and (110×107) hybrids had the highest and the lowest mortality rate.https://isj02.unimore.it/index.php/ISJ/article/view/23gene expressionheat shock proteinsreal-time PCRsilkworm hybridsthermotolerance |
| spellingShingle | S F Mousavi S H Hosseini Moghaddam N Ghavi Hossein-Zadeh S Z Mirhosseini Gene expression of HSP90 and HSP70 in four silkworm hybrids (Bombyx mori L.) in response to severe thermal shock Invertebrate Survival Journal gene expression heat shock proteins real-time PCR silkworm hybrids thermotolerance |
| title | Gene expression of HSP90 and HSP70 in four silkworm hybrids (Bombyx mori L.) in response to severe thermal shock |
| title_full | Gene expression of HSP90 and HSP70 in four silkworm hybrids (Bombyx mori L.) in response to severe thermal shock |
| title_fullStr | Gene expression of HSP90 and HSP70 in four silkworm hybrids (Bombyx mori L.) in response to severe thermal shock |
| title_full_unstemmed | Gene expression of HSP90 and HSP70 in four silkworm hybrids (Bombyx mori L.) in response to severe thermal shock |
| title_short | Gene expression of HSP90 and HSP70 in four silkworm hybrids (Bombyx mori L.) in response to severe thermal shock |
| title_sort | gene expression of hsp90 and hsp70 in four silkworm hybrids bombyx mori l in response to severe thermal shock |
| topic | gene expression heat shock proteins real-time PCR silkworm hybrids thermotolerance |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/23 |
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