Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections
Abstract Objective Multi-drug resistance (MDR) has notably increased in community acquired uropathogens causing urinary tract infections (UTIs), predominantly Escherichia coli. Uropathogenic E. coli causes 80% of uncomplicated community acquired UTIs, particularly in pre-menopausal women. Considerin...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-024-03221-8 |
| _version_ | 1827321582784086016 |
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| author | Ayesha Khan Viqar Sayeed Saraf Fariha Siddiqui Tahira Batool Zobia Noreen Sundus Javed Aftab Ahmad Wadi B. Alonazi Muhammad Ibrahim Sandra Pucciarelli Habib Bokhari |
| author_facet | Ayesha Khan Viqar Sayeed Saraf Fariha Siddiqui Tahira Batool Zobia Noreen Sundus Javed Aftab Ahmad Wadi B. Alonazi Muhammad Ibrahim Sandra Pucciarelli Habib Bokhari |
| author_sort | Ayesha Khan |
| collection | DOAJ |
| description | Abstract Objective Multi-drug resistance (MDR) has notably increased in community acquired uropathogens causing urinary tract infections (UTIs), predominantly Escherichia coli. Uropathogenic E. coli causes 80% of uncomplicated community acquired UTIs, particularly in pre-menopausal women. Considering this high prevalence and the potential to spread antimicrobial resistant genes, the current study was conducted to investigate the presence of clinically important strains of E. coli in Pakistani women having uncomplicated cystitis and pyelonephritis. Women belonging to low-income groups were exclusively included in the study. Seventy-four isolates from urine samples were processed, phylotyped, and screened for the presence of two Single Nucleotide Polymorphisms (SNPs) particularly associated with a clinically important clonal group A of E. coli (CgA) followed by antibiotic susceptibility testing and genome sequence analysis. Results Phylogroup B2 was most prevalent in patients and 44% of isolates were positive for the presence of CgA specific SNPs in Fumarate hydratase and DNA gyrase subunit B genes. Antibiotic susceptibility testing showed widespread resistance to trimethoprim-sulfamethoxazole and extended-spectrum beta-lactamase production. The infection analysis revealed the phylogroup B2 to be more pathogenic as compared to the other groups. The genome sequence of E. coli strain U17 revealed genes encoding virulence, multidrug resistance, and host colonization mechanisms. Conclusions Our research findings not only validate the significant occurrence of multidrug-resistant clonal group A E. coli (CgA) in premenopausal Pakistani women suffering from cystitis and pyelonephritis but also reveal the presence of genes associated withvirulence, and drug efflux pumps. The detection of highly pathogenic, antimicrobial-resistant phylogroup B2 and CgA E. coli strains is likely to help in understanding the epidemiology of the pathogen and may ultimately help to reduce the impact of these strains on human health. Furthermore, the findings of this study will particularly help to reduce the prevalence of uncomplicated UTIs and the cost associated with their treatment in women belonging to low-income groups. |
| first_indexed | 2024-04-25T01:08:45Z |
| format | Article |
| id | doaj.art-d569851a467e477d9dfa481176f26b3c |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-25T01:08:45Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-d569851a467e477d9dfa481176f26b3c2024-03-10T12:07:14ZengBMCBMC Microbiology1471-21802024-03-0124111210.1186/s12866-024-03221-8Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infectionsAyesha Khan0Viqar Sayeed Saraf1Fariha Siddiqui2Tahira Batool3Zobia Noreen4Sundus Javed5Aftab Ahmad6Wadi B. Alonazi7Muhammad Ibrahim8Sandra Pucciarelli9Habib Bokhari10Microbiology and Public Health Laboratory, Department of Biosciences, COMSATS University IslamabadMicrobiology and Public Health Laboratory, Department of Biosciences, COMSATS University IslamabadDepartment of Biosciences, Shifa Tameer e Millat UniversityMicrobiology and Public Health Laboratory, Department of Biosciences, COMSATS University IslamabadMicrobiology and Public Health Laboratory, Department of Biosciences, COMSATS University IslamabadMicrobiology and Public Health Laboratory, Department of Biosciences, COMSATS University IslamabadDepartment of Microbiology, Kohsar University MurreeHealth Administration Department, College of Business Administration, King Saud UniversityDepartment of Microbiology, Kohsar University MurreeSchool of Biosciences and Veterinary Medicine, University of CamerinoDepartment of Microbiology, Kohsar University MurreeAbstract Objective Multi-drug resistance (MDR) has notably increased in community acquired uropathogens causing urinary tract infections (UTIs), predominantly Escherichia coli. Uropathogenic E. coli causes 80% of uncomplicated community acquired UTIs, particularly in pre-menopausal women. Considering this high prevalence and the potential to spread antimicrobial resistant genes, the current study was conducted to investigate the presence of clinically important strains of E. coli in Pakistani women having uncomplicated cystitis and pyelonephritis. Women belonging to low-income groups were exclusively included in the study. Seventy-four isolates from urine samples were processed, phylotyped, and screened for the presence of two Single Nucleotide Polymorphisms (SNPs) particularly associated with a clinically important clonal group A of E. coli (CgA) followed by antibiotic susceptibility testing and genome sequence analysis. Results Phylogroup B2 was most prevalent in patients and 44% of isolates were positive for the presence of CgA specific SNPs in Fumarate hydratase and DNA gyrase subunit B genes. Antibiotic susceptibility testing showed widespread resistance to trimethoprim-sulfamethoxazole and extended-spectrum beta-lactamase production. The infection analysis revealed the phylogroup B2 to be more pathogenic as compared to the other groups. The genome sequence of E. coli strain U17 revealed genes encoding virulence, multidrug resistance, and host colonization mechanisms. Conclusions Our research findings not only validate the significant occurrence of multidrug-resistant clonal group A E. coli (CgA) in premenopausal Pakistani women suffering from cystitis and pyelonephritis but also reveal the presence of genes associated withvirulence, and drug efflux pumps. The detection of highly pathogenic, antimicrobial-resistant phylogroup B2 and CgA E. coli strains is likely to help in understanding the epidemiology of the pathogen and may ultimately help to reduce the impact of these strains on human health. Furthermore, the findings of this study will particularly help to reduce the prevalence of uncomplicated UTIs and the cost associated with their treatment in women belonging to low-income groups.https://doi.org/10.1186/s12866-024-03221-8Urinary tract infectionsLow-income groupPremenopausal womenE. ColiPhylotypesGalleria infection model |
| spellingShingle | Ayesha Khan Viqar Sayeed Saraf Fariha Siddiqui Tahira Batool Zobia Noreen Sundus Javed Aftab Ahmad Wadi B. Alonazi Muhammad Ibrahim Sandra Pucciarelli Habib Bokhari Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections BMC Microbiology Urinary tract infections Low-income group Premenopausal women E. Coli Phylotypes Galleria infection model |
| title | Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections |
| title_full | Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections |
| title_fullStr | Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections |
| title_full_unstemmed | Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections |
| title_short | Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections |
| title_sort | multidrug resistance among uropathogenic clonal group a e coli isolates from pakistani women with uncomplicated urinary tract infections |
| topic | Urinary tract infections Low-income group Premenopausal women E. Coli Phylotypes Galleria infection model |
| url | https://doi.org/10.1186/s12866-024-03221-8 |
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