Unravelling staphylococcal small-colony variants in cardiac implantable electronic device infections: clinical characteristics, management, and genomic insights
ObjectivesStaphylococcal small-colony variants (SCVs) are common in cardiac implantable electronic device (CIED) infections. This is the first retrospective and multi-case study on CIED infections due to staphylococcal SCVs, aiming to provide a theoretical basis for the clinical management of CIED a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-01-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1321626/full |
| _version_ | 1797362354478907392 |
|---|---|
| author | Si Liu Hongbin Chen Fangjie Xu Fengning Chen Yuyao Yin Xiaoyang Zhang Shangyu Tu Hui Wang |
| author_facet | Si Liu Hongbin Chen Fangjie Xu Fengning Chen Yuyao Yin Xiaoyang Zhang Shangyu Tu Hui Wang |
| author_sort | Si Liu |
| collection | DOAJ |
| description | ObjectivesStaphylococcal small-colony variants (SCVs) are common in cardiac implantable electronic device (CIED) infections. This is the first retrospective and multi-case study on CIED infections due to staphylococcal SCVs, aiming to provide a theoretical basis for the clinical management of CIED and device-related infections caused by staphylococcal SCVs.MethodsNinety patients with culture positive CIED infections were enrolled between 2021 and 2022. We compared the demographic and clinical characteristics of patients with and without SCVs and performed genomic studies on SCVs isolates.ResultsCompared to patients without SCVs, those with SCVs had a longer primary pacemaker implantation time and were more likely to have a history of device replacement and infection. They showed upregulated inflammatory indicators, especially higher NEUT% (52.6 vs. 26.8%, P = 0.032) and they had longer hospital stays (median 13 vs. 12 days, P = 0.012). Comparative genomics analysis was performed on Staphylococcus epidermidis wild-type and SCVs. Some genes were identified, including aap, genes encoding adhesin, CHAP domain-containing protein, LPXTG cell wall anchor domain-containing protein, and YSIRK-type signal peptide-containing protein.ConclusionStaphylococcal SCVs affect the clinical characteristics of CIED infections. The process of staphylococcal SCVs adherence, biofilm formation, and interaction with neutrophils play a vital role. |
| first_indexed | 2024-03-08T16:06:49Z |
| format | Article |
| id | doaj.art-d56e9e9a682e44a68d529441e67158c9 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-08T16:06:49Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d56e9e9a682e44a68d529441e67158c92024-01-08T05:42:49ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-01-011310.3389/fcimb.2023.13216261321626Unravelling staphylococcal small-colony variants in cardiac implantable electronic device infections: clinical characteristics, management, and genomic insightsSi Liu0Hongbin Chen1Fangjie Xu2Fengning Chen3Yuyao Yin4Xiaoyang Zhang5Shangyu Tu6Hui Wang7Department of Clinical Laboratory, Peking University People’s Hospital, Beijing, ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, ChinaDepartment of Clinical Laboratory, Urumqi Friendship Hospital, Urumqi, ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, ChinaDepartment of Clinical Laboratory, Peking University People’s Hospital, Beijing, ChinaObjectivesStaphylococcal small-colony variants (SCVs) are common in cardiac implantable electronic device (CIED) infections. This is the first retrospective and multi-case study on CIED infections due to staphylococcal SCVs, aiming to provide a theoretical basis for the clinical management of CIED and device-related infections caused by staphylococcal SCVs.MethodsNinety patients with culture positive CIED infections were enrolled between 2021 and 2022. We compared the demographic and clinical characteristics of patients with and without SCVs and performed genomic studies on SCVs isolates.ResultsCompared to patients without SCVs, those with SCVs had a longer primary pacemaker implantation time and were more likely to have a history of device replacement and infection. They showed upregulated inflammatory indicators, especially higher NEUT% (52.6 vs. 26.8%, P = 0.032) and they had longer hospital stays (median 13 vs. 12 days, P = 0.012). Comparative genomics analysis was performed on Staphylococcus epidermidis wild-type and SCVs. Some genes were identified, including aap, genes encoding adhesin, CHAP domain-containing protein, LPXTG cell wall anchor domain-containing protein, and YSIRK-type signal peptide-containing protein.ConclusionStaphylococcal SCVs affect the clinical characteristics of CIED infections. The process of staphylococcal SCVs adherence, biofilm formation, and interaction with neutrophils play a vital role.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1321626/fullsmall-colony variants (SCVs)Staphylococcus epidermidisCardiac implantable electronic device (CIED) infectionwhole-genome sequencing (WGS)epidemiological survey |
| spellingShingle | Si Liu Hongbin Chen Fangjie Xu Fengning Chen Yuyao Yin Xiaoyang Zhang Shangyu Tu Hui Wang Unravelling staphylococcal small-colony variants in cardiac implantable electronic device infections: clinical characteristics, management, and genomic insights Frontiers in Cellular and Infection Microbiology small-colony variants (SCVs) Staphylococcus epidermidis Cardiac implantable electronic device (CIED) infection whole-genome sequencing (WGS) epidemiological survey |
| title | Unravelling staphylococcal small-colony variants in cardiac implantable electronic device infections: clinical characteristics, management, and genomic insights |
| title_full | Unravelling staphylococcal small-colony variants in cardiac implantable electronic device infections: clinical characteristics, management, and genomic insights |
| title_fullStr | Unravelling staphylococcal small-colony variants in cardiac implantable electronic device infections: clinical characteristics, management, and genomic insights |
| title_full_unstemmed | Unravelling staphylococcal small-colony variants in cardiac implantable electronic device infections: clinical characteristics, management, and genomic insights |
| title_short | Unravelling staphylococcal small-colony variants in cardiac implantable electronic device infections: clinical characteristics, management, and genomic insights |
| title_sort | unravelling staphylococcal small colony variants in cardiac implantable electronic device infections clinical characteristics management and genomic insights |
| topic | small-colony variants (SCVs) Staphylococcus epidermidis Cardiac implantable electronic device (CIED) infection whole-genome sequencing (WGS) epidemiological survey |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1321626/full |
| work_keys_str_mv | AT siliu unravellingstaphylococcalsmallcolonyvariantsincardiacimplantableelectronicdeviceinfectionsclinicalcharacteristicsmanagementandgenomicinsights AT hongbinchen unravellingstaphylococcalsmallcolonyvariantsincardiacimplantableelectronicdeviceinfectionsclinicalcharacteristicsmanagementandgenomicinsights AT fangjiexu unravellingstaphylococcalsmallcolonyvariantsincardiacimplantableelectronicdeviceinfectionsclinicalcharacteristicsmanagementandgenomicinsights AT fengningchen unravellingstaphylococcalsmallcolonyvariantsincardiacimplantableelectronicdeviceinfectionsclinicalcharacteristicsmanagementandgenomicinsights AT yuyaoyin unravellingstaphylococcalsmallcolonyvariantsincardiacimplantableelectronicdeviceinfectionsclinicalcharacteristicsmanagementandgenomicinsights AT xiaoyangzhang unravellingstaphylococcalsmallcolonyvariantsincardiacimplantableelectronicdeviceinfectionsclinicalcharacteristicsmanagementandgenomicinsights AT shangyutu unravellingstaphylococcalsmallcolonyvariantsincardiacimplantableelectronicdeviceinfectionsclinicalcharacteristicsmanagementandgenomicinsights AT huiwang unravellingstaphylococcalsmallcolonyvariantsincardiacimplantableelectronicdeviceinfectionsclinicalcharacteristicsmanagementandgenomicinsights |