Time-gated detection of protein-protein interactions with transcriptional readout
Transcriptional assays, such as yeast two-hybrid and TANGO, that convert transient protein-protein interactions (PPIs) into stable expression of transgenes are powerful tools for PPI discovery, screens, and analysis of cell populations. However, such assays often have high background and lose inform...
Main Authors: | Min Woo Kim, Wenjing Wang, Mateo I Sanchez, Robert Coukos, Mark von Zastrow, Alice Y Ting |
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Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2017-11-01
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Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/30233 |
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