Augmented myocardial methionine-enkephalin in a murine model of cardiac angiotensin II-overexpression
Introduction. The endogenous opioid system has been reported to interact with both the cardiac sympathetic and renin-angiotensin systems in exerting a local regulatory action on the heart.The goal of this investigation was to examine how cardiac levels of enkephalin production are altered in the dev...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2007-12-01
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| Series: | Journal of the Renin-Angiotensin-Aldosterone System |
| Online Access: | https://doi.org/10.3317/jraas.2007.030 |
| _version_ | 1797282045465985024 |
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| author | Olivier WV van den Brink Lea M Durham Delbridge Thierry Pedrazzini Franklin L Rosenfeldt Salvatore Pepe |
| author_facet | Olivier WV van den Brink Lea M Durham Delbridge Thierry Pedrazzini Franklin L Rosenfeldt Salvatore Pepe |
| author_sort | Olivier WV van den Brink |
| collection | DOAJ |
| description | Introduction. The endogenous opioid system has been reported to interact with both the cardiac sympathetic and renin-angiotensin systems in exerting a local regulatory action on the heart.The goal of this investigation was to examine how cardiac levels of enkephalin production are altered in the development of normotensive primary hypertrophy due to elevated intra-cardiac angiotensin II (Ang II) production. Methods. Atrial and ventricular methionine-enkephalin (ME) levels were measured by quantitative radioimmunoassay in 14 and 28-week-old male transgenic mice (TG1306/1R) and control mice.The TG1306/1R exhibit cardiac specific Ang II overexpression and cardiac hypertrophy, but not hypertension. Results. TG1306/1R mice had significantly higher heart/body weight ratios H (15—20%) than control littermates at both 14 (p=0.02) and 28 weeks (p=0.04). Relative to controls, ME content was significantly elevated (approximately twofold) in atria and ventricles in the older 28-week TG1306/1R mice only. A significant inverse correlation between heart size and ME level was observed for 28-week TG1306/1R only. Conclusions. We have provided evidence that a marked elevation of myocardial enkephalin level is observed in the established (but not early) phase of cardiac hypertrophy associated with cardiac-specific Ang II-overexpression.This study identifies a potentially important relationship between two endogenous peptidergic signalling systems involved in the regulation of growth and function of the hypertrophic heart. |
| first_indexed | 2024-03-07T17:06:18Z |
| format | Article |
| id | doaj.art-d5736b7dac9c40c3a6191eda17593872 |
| institution | Directory Open Access Journal |
| issn | 1470-3203 |
| language | English |
| last_indexed | 2024-03-07T17:06:18Z |
| publishDate | 2007-12-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Journal of the Renin-Angiotensin-Aldosterone System |
| spelling | doaj.art-d5736b7dac9c40c3a6191eda175938722024-03-03T02:38:23ZengSAGE PublicationsJournal of the Renin-Angiotensin-Aldosterone System1470-32032007-12-01810.3317/jraas.2007.030Augmented myocardial methionine-enkephalin in a murine model of cardiac angiotensin II-overexpressionOlivier WV van den BrinkLea M Durham DelbridgeThierry PedrazziniFranklin L RosenfeldtSalvatore PepeIntroduction. The endogenous opioid system has been reported to interact with both the cardiac sympathetic and renin-angiotensin systems in exerting a local regulatory action on the heart.The goal of this investigation was to examine how cardiac levels of enkephalin production are altered in the development of normotensive primary hypertrophy due to elevated intra-cardiac angiotensin II (Ang II) production. Methods. Atrial and ventricular methionine-enkephalin (ME) levels were measured by quantitative radioimmunoassay in 14 and 28-week-old male transgenic mice (TG1306/1R) and control mice.The TG1306/1R exhibit cardiac specific Ang II overexpression and cardiac hypertrophy, but not hypertension. Results. TG1306/1R mice had significantly higher heart/body weight ratios H (15—20%) than control littermates at both 14 (p=0.02) and 28 weeks (p=0.04). Relative to controls, ME content was significantly elevated (approximately twofold) in atria and ventricles in the older 28-week TG1306/1R mice only. A significant inverse correlation between heart size and ME level was observed for 28-week TG1306/1R only. Conclusions. We have provided evidence that a marked elevation of myocardial enkephalin level is observed in the established (but not early) phase of cardiac hypertrophy associated with cardiac-specific Ang II-overexpression.This study identifies a potentially important relationship between two endogenous peptidergic signalling systems involved in the regulation of growth and function of the hypertrophic heart.https://doi.org/10.3317/jraas.2007.030 |
| spellingShingle | Olivier WV van den Brink Lea M Durham Delbridge Thierry Pedrazzini Franklin L Rosenfeldt Salvatore Pepe Augmented myocardial methionine-enkephalin in a murine model of cardiac angiotensin II-overexpression Journal of the Renin-Angiotensin-Aldosterone System |
| title | Augmented myocardial methionine-enkephalin in a murine model of cardiac angiotensin II-overexpression |
| title_full | Augmented myocardial methionine-enkephalin in a murine model of cardiac angiotensin II-overexpression |
| title_fullStr | Augmented myocardial methionine-enkephalin in a murine model of cardiac angiotensin II-overexpression |
| title_full_unstemmed | Augmented myocardial methionine-enkephalin in a murine model of cardiac angiotensin II-overexpression |
| title_short | Augmented myocardial methionine-enkephalin in a murine model of cardiac angiotensin II-overexpression |
| title_sort | augmented myocardial methionine enkephalin in a murine model of cardiac angiotensin ii overexpression |
| url | https://doi.org/10.3317/jraas.2007.030 |
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