Efficient induction and rapid identification of haploid grains in tetraploid wheat by editing genes TtMTL and pyramiding anthocyanin markers

Efficient induction and rapid identification of haploid grains in tetraploid wheat by editing genes TtMTL and pyramiding anthocyanin markers

Doubled haploid (DH) technology provides an effective way to generate homozygous genetic and breeding materials over a short period of time. We produced three types of homozygous TtMTL gene-edited mutants (mtl-a, mtl-b, and mtl-ab) by CRISPR/Cas9 in durum wheat. PCR restriction enzymes and sequencin...

Full description

Bibliographic Details
Main Authors: Yanan Chang, Huali Tang, Surong Wang, Xi Li, Peipei Huang, Jiahui Zhang, Ke Wang, Yueming Yan, Xingguo Ye
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-03-01
Series:Frontiers in Plant Science
Subjects:
durum wheat
TtMTL genes
CRISPR/Cas9
haploid induction
purple embryo
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2024.1346364/full

Similar Items

Similar Items