Critical Steps of Plasmodium falciparum Ookinete Maturation
The egress and fertilization of Plasmodium gametes and development of a motile ookinete are the first crucial steps that mediate the successful transmission of the malaria parasites from humans to the Anopheles vector. However, limited information exists about the cell biology and regulation of this...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00269/full |
| _version_ | 1818925415470727168 |
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| author | Giulia Siciliano Giulia Costa Pablo Suárez-Cortés Pablo Suárez-Cortés Angelo Valleriani Angelo Valleriani Pietro Alano Elena A. Levashina |
| author_facet | Giulia Siciliano Giulia Costa Pablo Suárez-Cortés Pablo Suárez-Cortés Angelo Valleriani Angelo Valleriani Pietro Alano Elena A. Levashina |
| author_sort | Giulia Siciliano |
| collection | DOAJ |
| description | The egress and fertilization of Plasmodium gametes and development of a motile ookinete are the first crucial steps that mediate the successful transmission of the malaria parasites from humans to the Anopheles vector. However, limited information exists about the cell biology and regulation of this process. Technical impediments in the establishment of in vitro conditions for ookinete maturation in Plasmodium falciparum and other human malaria parasites further constrain a detailed characterization of ookinete maturation. Here, using fluorescence microscopy and immunolabeling, we compared P. falciparum ookinete maturation in Anopheles coluzzii mosquitoes in vivo and in cell culture in vitro. Our results identified two critical steps in ookinete maturation that are regulated by distinct mosquito factors, thereby highlighting the role of the mosquito environment in the transmission efficiency of malaria parasites. |
| first_indexed | 2024-12-20T02:40:52Z |
| format | Article |
| id | doaj.art-d5760d93eb7144cfa4ca709f83e9c3de |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T02:40:52Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d5760d93eb7144cfa4ca709f83e9c3de2022-12-21T19:56:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-03-011110.3389/fmicb.2020.00269513816Critical Steps of Plasmodium falciparum Ookinete MaturationGiulia Siciliano0Giulia Costa1Pablo Suárez-Cortés2Pablo Suárez-Cortés3Angelo Valleriani4Angelo Valleriani5Pietro Alano6Elena A. Levashina7Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, ItalyVector Biology, Max Planck Institute for Infection Biology, Berlin, GermanyDipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, ItalyVector Biology, Max Planck Institute for Infection Biology, Berlin, GermanyVector Biology, Max Planck Institute for Infection Biology, Berlin, GermanyDepartment of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, GermanyDipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, ItalyVector Biology, Max Planck Institute for Infection Biology, Berlin, GermanyThe egress and fertilization of Plasmodium gametes and development of a motile ookinete are the first crucial steps that mediate the successful transmission of the malaria parasites from humans to the Anopheles vector. However, limited information exists about the cell biology and regulation of this process. Technical impediments in the establishment of in vitro conditions for ookinete maturation in Plasmodium falciparum and other human malaria parasites further constrain a detailed characterization of ookinete maturation. Here, using fluorescence microscopy and immunolabeling, we compared P. falciparum ookinete maturation in Anopheles coluzzii mosquitoes in vivo and in cell culture in vitro. Our results identified two critical steps in ookinete maturation that are regulated by distinct mosquito factors, thereby highlighting the role of the mosquito environment in the transmission efficiency of malaria parasites.https://www.frontiersin.org/article/10.3389/fmicb.2020.00269/fullmalaria parasite transmissionPlasmodium falciparumAnopheles coluzziiookinete maturationtime course of zygote developmentdevelopmental bottleneck |
| spellingShingle | Giulia Siciliano Giulia Costa Pablo Suárez-Cortés Pablo Suárez-Cortés Angelo Valleriani Angelo Valleriani Pietro Alano Elena A. Levashina Critical Steps of Plasmodium falciparum Ookinete Maturation Frontiers in Microbiology malaria parasite transmission Plasmodium falciparum Anopheles coluzzii ookinete maturation time course of zygote development developmental bottleneck |
| title | Critical Steps of Plasmodium falciparum Ookinete Maturation |
| title_full | Critical Steps of Plasmodium falciparum Ookinete Maturation |
| title_fullStr | Critical Steps of Plasmodium falciparum Ookinete Maturation |
| title_full_unstemmed | Critical Steps of Plasmodium falciparum Ookinete Maturation |
| title_short | Critical Steps of Plasmodium falciparum Ookinete Maturation |
| title_sort | critical steps of plasmodium falciparum ookinete maturation |
| topic | malaria parasite transmission Plasmodium falciparum Anopheles coluzzii ookinete maturation time course of zygote development developmental bottleneck |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00269/full |
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