Phytochemical Characterization, Antioxidant and Anti-Inflammatory Effects of <i>Cleome arabica</i> L. Fruits Extract against Formalin Induced Chronic Inflammation in Female Wistar Rat: Biochemical, Histological, and In Silico Studies
In recent decades, the use of herbs and plants has been of great interest, as they have been the sources of natural products, commonly named as bioactive compounds. In specific, the natural compounds from the Capparaceae family which has been proved to have antioxidant, anti-inflammatory, antimicrob...
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2022-12-01
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| author | Ikram Allagui Mabrouk Horchani Nourhene Zammel Maroua Jalouli Abdelfatteh Elfeki Choumous Kallel Lamjed Mansour Salah Alwasel Abdel Halim Harrath Hichem Ben Jannet Mohamed Salah Allagui Kheiria Hcini |
| author_facet | Ikram Allagui Mabrouk Horchani Nourhene Zammel Maroua Jalouli Abdelfatteh Elfeki Choumous Kallel Lamjed Mansour Salah Alwasel Abdel Halim Harrath Hichem Ben Jannet Mohamed Salah Allagui Kheiria Hcini |
| author_sort | Ikram Allagui |
| collection | DOAJ |
| description | In recent decades, the use of herbs and plants has been of great interest, as they have been the sources of natural products, commonly named as bioactive compounds. In specific, the natural compounds from the Capparaceae family which has been proved to have antioxidant, anti-inflammatory, antimicrobial and anti-carcinogenic activities, by several studies. <i>Cleome arabica</i> L. (CA) specie is the most used medicinal plants in Tunisia and elsewhere in North African countries for treatment of various diseases including diabetes, rheumatism, inflammation, cancer, and digestive disorders. The current work was undertaken to estimate the total phenolic, flavonoid and condensed tannin contents, to identify and quantify the polyphenolic compounds, and to evaluate the antioxidant and the anti-inflammatory proprieties of CA fruits extract against formalin induced chronic inflammation in Female Wistar rats. In fact, the antioxidant activity was tested by Diphenyl-1-Picrylhydrazyl free radical scavenging (DPPH), Ferric reducing antioxidant power (FRAP) and Nitric Oxide radical (NO·). Anti-inflammatory effect of fruits extract was examined using formalin (2%) induced paw edema in rats. Molecular docking tools were used to investigate the interaction of some compounds from CA fruits extract with the cyclooxygenase-2 (COX-2) target protein. Our results showed that, the total phenolic, flavonoid and tannins contents, which were assessed by the Folin-Ciocalteu, Quercetin, and Catechin methods, respectively, were 230.22 mg gallic acid equivalent/g dry weight (mg GAE/g DW), 55.08 mg quercetin equivalent/g dry weight (QE/g DW) and 15.17 mg catechin equivalents/g dry weight (CatE/g DW), respectively. HPLC analysis revealed the presence of five polyphenolic compounds whose catechin was found to be the most abundant compounds. The antioxidant activity of extract was quantified by DPPH, FRAP and NO· tests and IC<sub>50</sub> reached the values of 3.346 mg/mL, 2.306 and 0.023 mg/mL, respectively. Cleome fruits ameliorated the histological integrity of the skin and alleviated the disruptions in hematological parameters (WBC, LYM, RBC, and HGB), inflammatory cytokines (IL-1β, IL-6, TNF-α), C-reactive protein, and some oxidative stress markers (TBARS (−49%) and AOPP (−42%) levels, SOD (+33%) and GPx (+75%) activities, and GSH (+49%) content) induced by formalin injection. Moreover, the in-silico investigation had shown that CA fruits extract compounds have a stronger interaction with COX-2 active site, more than the reference drug “indomethacin” (two H-bonds). Our research gives pharmacological backing to the healthcare utilization of Cleome plant in the treatment of inflammatory diseases and oxidative harm. |
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| spelling | doaj.art-d578c35941a94ca28c7f408a1a278f882023-11-30T22:56:06ZengMDPI AGMolecules1420-30492022-12-012812610.3390/molecules28010026Phytochemical Characterization, Antioxidant and Anti-Inflammatory Effects of <i>Cleome arabica</i> L. Fruits Extract against Formalin Induced Chronic Inflammation in Female Wistar Rat: Biochemical, Histological, and In Silico StudiesIkram Allagui0Mabrouk Horchani1Nourhene Zammel2Maroua Jalouli3Abdelfatteh Elfeki4Choumous Kallel5Lamjed Mansour6Salah Alwasel7Abdel Halim Harrath8Hichem Ben Jannet9Mohamed Salah Allagui10Kheiria Hcini11Laboratory of Animal Physiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, Sfax 3052, TunisiaLaboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11Es39), Medicinal Chemistry and Natural Products, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, Monastir 5000, TunisiaLaboratory of Histo-Embryology and Cytogenetics, Medicine Faculty of Sfax, University of Sfax, Sfax 3029, TunisiaDepartment of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaLaboratory of Animal Physiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, Sfax 3052, TunisiaLaboratory of Hematology, University of Sfax, CHU Habib Bourguiba, Sfax 3029, TunisiaDepartment of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaLaboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11Es39), Medicinal Chemistry and Natural Products, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, Monastir 5000, TunisiaLaboratory of Animal Physiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, Sfax 3052, TunisiaBiodiversity, Biotechnology and Climate Change Laboratory (LR11ES09), Department of Life Sciences, Faculty of Science of Tunis, University of Tunis El Manar, Tunis 2092, TunisiaIn recent decades, the use of herbs and plants has been of great interest, as they have been the sources of natural products, commonly named as bioactive compounds. In specific, the natural compounds from the Capparaceae family which has been proved to have antioxidant, anti-inflammatory, antimicrobial and anti-carcinogenic activities, by several studies. <i>Cleome arabica</i> L. (CA) specie is the most used medicinal plants in Tunisia and elsewhere in North African countries for treatment of various diseases including diabetes, rheumatism, inflammation, cancer, and digestive disorders. The current work was undertaken to estimate the total phenolic, flavonoid and condensed tannin contents, to identify and quantify the polyphenolic compounds, and to evaluate the antioxidant and the anti-inflammatory proprieties of CA fruits extract against formalin induced chronic inflammation in Female Wistar rats. In fact, the antioxidant activity was tested by Diphenyl-1-Picrylhydrazyl free radical scavenging (DPPH), Ferric reducing antioxidant power (FRAP) and Nitric Oxide radical (NO·). Anti-inflammatory effect of fruits extract was examined using formalin (2%) induced paw edema in rats. Molecular docking tools were used to investigate the interaction of some compounds from CA fruits extract with the cyclooxygenase-2 (COX-2) target protein. Our results showed that, the total phenolic, flavonoid and tannins contents, which were assessed by the Folin-Ciocalteu, Quercetin, and Catechin methods, respectively, were 230.22 mg gallic acid equivalent/g dry weight (mg GAE/g DW), 55.08 mg quercetin equivalent/g dry weight (QE/g DW) and 15.17 mg catechin equivalents/g dry weight (CatE/g DW), respectively. HPLC analysis revealed the presence of five polyphenolic compounds whose catechin was found to be the most abundant compounds. The antioxidant activity of extract was quantified by DPPH, FRAP and NO· tests and IC<sub>50</sub> reached the values of 3.346 mg/mL, 2.306 and 0.023 mg/mL, respectively. Cleome fruits ameliorated the histological integrity of the skin and alleviated the disruptions in hematological parameters (WBC, LYM, RBC, and HGB), inflammatory cytokines (IL-1β, IL-6, TNF-α), C-reactive protein, and some oxidative stress markers (TBARS (−49%) and AOPP (−42%) levels, SOD (+33%) and GPx (+75%) activities, and GSH (+49%) content) induced by formalin injection. Moreover, the in-silico investigation had shown that CA fruits extract compounds have a stronger interaction with COX-2 active site, more than the reference drug “indomethacin” (two H-bonds). Our research gives pharmacological backing to the healthcare utilization of Cleome plant in the treatment of inflammatory diseases and oxidative harm.https://www.mdpi.com/1420-3049/28/1/26<i>Cleome arabica</i> L.phenolic compoundsHPLCantioxidant activitypotential anti-inflammatorychronic inflammation |
| spellingShingle | Ikram Allagui Mabrouk Horchani Nourhene Zammel Maroua Jalouli Abdelfatteh Elfeki Choumous Kallel Lamjed Mansour Salah Alwasel Abdel Halim Harrath Hichem Ben Jannet Mohamed Salah Allagui Kheiria Hcini Phytochemical Characterization, Antioxidant and Anti-Inflammatory Effects of <i>Cleome arabica</i> L. Fruits Extract against Formalin Induced Chronic Inflammation in Female Wistar Rat: Biochemical, Histological, and In Silico Studies Molecules <i>Cleome arabica</i> L. phenolic compounds HPLC antioxidant activity potential anti-inflammatory chronic inflammation |
| title | Phytochemical Characterization, Antioxidant and Anti-Inflammatory Effects of <i>Cleome arabica</i> L. Fruits Extract against Formalin Induced Chronic Inflammation in Female Wistar Rat: Biochemical, Histological, and In Silico Studies |
| title_full | Phytochemical Characterization, Antioxidant and Anti-Inflammatory Effects of <i>Cleome arabica</i> L. Fruits Extract against Formalin Induced Chronic Inflammation in Female Wistar Rat: Biochemical, Histological, and In Silico Studies |
| title_fullStr | Phytochemical Characterization, Antioxidant and Anti-Inflammatory Effects of <i>Cleome arabica</i> L. Fruits Extract against Formalin Induced Chronic Inflammation in Female Wistar Rat: Biochemical, Histological, and In Silico Studies |
| title_full_unstemmed | Phytochemical Characterization, Antioxidant and Anti-Inflammatory Effects of <i>Cleome arabica</i> L. Fruits Extract against Formalin Induced Chronic Inflammation in Female Wistar Rat: Biochemical, Histological, and In Silico Studies |
| title_short | Phytochemical Characterization, Antioxidant and Anti-Inflammatory Effects of <i>Cleome arabica</i> L. Fruits Extract against Formalin Induced Chronic Inflammation in Female Wistar Rat: Biochemical, Histological, and In Silico Studies |
| title_sort | phytochemical characterization antioxidant and anti inflammatory effects of i cleome arabica i l fruits extract against formalin induced chronic inflammation in female wistar rat biochemical histological and in silico studies |
| topic | <i>Cleome arabica</i> L. phenolic compounds HPLC antioxidant activity potential anti-inflammatory chronic inflammation |
| url | https://www.mdpi.com/1420-3049/28/1/26 |
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