Functional characterization of thermotolerant microbial consortium for lignocellulolytic enzymes with central role of Firmicutes in rice straw depolymerization
Abstract Rice (Oryza sativa L.) straw, an agricultural waste of high yield, is a sustainable source of fermentable sugars for biofuel and other chemicals. However, it shows recalcitrance to microbial catalysed depolymerization. We herein describe development of thermotolerant microbial consortium (R...
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-82163-x |
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| author | Parmeshwar V. Gavande Arijita Basak Subhajit Sen Khusboo Lepcha Nensina Murmu Vijeta Rai Deepika Mazumdar Shyama Prasad Saha Vaskar Das Shilpi Ghosh |
| author_facet | Parmeshwar V. Gavande Arijita Basak Subhajit Sen Khusboo Lepcha Nensina Murmu Vijeta Rai Deepika Mazumdar Shyama Prasad Saha Vaskar Das Shilpi Ghosh |
| author_sort | Parmeshwar V. Gavande |
| collection | DOAJ |
| description | Abstract Rice (Oryza sativa L.) straw, an agricultural waste of high yield, is a sustainable source of fermentable sugars for biofuel and other chemicals. However, it shows recalcitrance to microbial catalysed depolymerization. We herein describe development of thermotolerant microbial consortium (RSV) from vermicompost with ability to degrade rice straw and analysis of its metagenome for bacterial diversity, and lignocellulolytic carbohydrate active enzymes (CAZymes) and their phylogenetic affiliations. RSV secretome exhibited cellulases and hemicellulases with higher activity at 60 °C. It catalysed depolymerization of chemical pretreated rice straw as revealed by scanning electron microscopy and saccharification yield of 460 mg g−1 rice straw. Microbial diversity of RSV was distinct from other compost habitats, with predominance of members of phyla Firmicutes, Proteobacteria and Bacteroidetes; and Pseudoclostridium, Thermoanaerobacterium, Chelatococcus and Algoriphagus being most abundant genera. RSV harboured 1389 CAZyme encoding ORFs of glycoside hydrolase, carbohydrate esterase, glycosyl transferase, carbohydrate binding module and auxiliary activity functions. Microorganisms of Firmicutes showed central role in lignocellulose deconstruction with importance in hemicellulose degradation; whereas representatives of Proteobacteria and Bacteroidetes contributed to cellulose and lignin degradation, respectively. RSV consortium could be a resource for mining thermotolerant cellulolytic bacteria or enzymes and studying their synergism in deconstruction of chemically pretreated rice straw. |
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| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T09:48:02Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-d57a92867ee747098c7a2bdfb10fdf7c2022-12-21T19:44:41ZengNature PortfolioScientific Reports2045-23222021-02-0111111310.1038/s41598-021-82163-xFunctional characterization of thermotolerant microbial consortium for lignocellulolytic enzymes with central role of Firmicutes in rice straw depolymerizationParmeshwar V. Gavande0Arijita Basak1Subhajit Sen2Khusboo Lepcha3Nensina Murmu4Vijeta Rai5Deepika Mazumdar6Shyama Prasad Saha7Vaskar Das8Shilpi Ghosh9Department of Biotechnology, University of North BengalDepartment of Biotechnology, University of North BengalDepartment of Biotechnology, University of North BengalDepartment of Biotechnology, University of North BengalDepartment of Biotechnology, University of North BengalDepartment of Biotechnology, University of North BengalDepartment of Biotechnology, University of North BengalDepartment of Biotechnology, University of North BengalDepartment of Biotechnology, University of North BengalDepartment of Biotechnology, University of North BengalAbstract Rice (Oryza sativa L.) straw, an agricultural waste of high yield, is a sustainable source of fermentable sugars for biofuel and other chemicals. However, it shows recalcitrance to microbial catalysed depolymerization. We herein describe development of thermotolerant microbial consortium (RSV) from vermicompost with ability to degrade rice straw and analysis of its metagenome for bacterial diversity, and lignocellulolytic carbohydrate active enzymes (CAZymes) and their phylogenetic affiliations. RSV secretome exhibited cellulases and hemicellulases with higher activity at 60 °C. It catalysed depolymerization of chemical pretreated rice straw as revealed by scanning electron microscopy and saccharification yield of 460 mg g−1 rice straw. Microbial diversity of RSV was distinct from other compost habitats, with predominance of members of phyla Firmicutes, Proteobacteria and Bacteroidetes; and Pseudoclostridium, Thermoanaerobacterium, Chelatococcus and Algoriphagus being most abundant genera. RSV harboured 1389 CAZyme encoding ORFs of glycoside hydrolase, carbohydrate esterase, glycosyl transferase, carbohydrate binding module and auxiliary activity functions. Microorganisms of Firmicutes showed central role in lignocellulose deconstruction with importance in hemicellulose degradation; whereas representatives of Proteobacteria and Bacteroidetes contributed to cellulose and lignin degradation, respectively. RSV consortium could be a resource for mining thermotolerant cellulolytic bacteria or enzymes and studying their synergism in deconstruction of chemically pretreated rice straw.https://doi.org/10.1038/s41598-021-82163-x |
| spellingShingle | Parmeshwar V. Gavande Arijita Basak Subhajit Sen Khusboo Lepcha Nensina Murmu Vijeta Rai Deepika Mazumdar Shyama Prasad Saha Vaskar Das Shilpi Ghosh Functional characterization of thermotolerant microbial consortium for lignocellulolytic enzymes with central role of Firmicutes in rice straw depolymerization Scientific Reports |
| title | Functional characterization of thermotolerant microbial consortium for lignocellulolytic enzymes with central role of Firmicutes in rice straw depolymerization |
| title_full | Functional characterization of thermotolerant microbial consortium for lignocellulolytic enzymes with central role of Firmicutes in rice straw depolymerization |
| title_fullStr | Functional characterization of thermotolerant microbial consortium for lignocellulolytic enzymes with central role of Firmicutes in rice straw depolymerization |
| title_full_unstemmed | Functional characterization of thermotolerant microbial consortium for lignocellulolytic enzymes with central role of Firmicutes in rice straw depolymerization |
| title_short | Functional characterization of thermotolerant microbial consortium for lignocellulolytic enzymes with central role of Firmicutes in rice straw depolymerization |
| title_sort | functional characterization of thermotolerant microbial consortium for lignocellulolytic enzymes with central role of firmicutes in rice straw depolymerization |
| url | https://doi.org/10.1038/s41598-021-82163-x |
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