Comparison of Ultra-Magnifying Endocytoscopic and Hematoxylin-Eosin-Stained Images of Lung Specimens
Endocytoscopy enables real-time observation of lesions at ultra-magnification. In the gastrointestinal and respiratory fields, endocytoscopic images are similar to hematoxylin-eosin-stained images. This study aimed to compare the nuclear features of pulmonary lesions in endocytoscopic and hematoxyli...
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MDPI AG
2023-03-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/5/1003 |
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| author | Misato Kobayashi Noriaki Kurimoto Ryosuke Tanino Yohei Shiratsuki Takae Okuno Mika Nakao Takamasa Hotta Yukari Tsubata Makoto Nagasaki Takashi Nishisaka Takeshi Isobe |
| author_facet | Misato Kobayashi Noriaki Kurimoto Ryosuke Tanino Yohei Shiratsuki Takae Okuno Mika Nakao Takamasa Hotta Yukari Tsubata Makoto Nagasaki Takashi Nishisaka Takeshi Isobe |
| author_sort | Misato Kobayashi |
| collection | DOAJ |
| description | Endocytoscopy enables real-time observation of lesions at ultra-magnification. In the gastrointestinal and respiratory fields, endocytoscopic images are similar to hematoxylin-eosin-stained images. This study aimed to compare the nuclear features of pulmonary lesions in endocytoscopic and hematoxylin-eosin-stained images. We performed an endocytoscopy to observe resected specimens of normal lung tissue and lesions. Nuclear features were extracted using ImageJ. We analyzed five nuclear features: nuclear number per area, mean nucleus area, median circularity, coefficient of variation of roundness, and median Voronoi area. We conducted dimensionality reduction analyses for these features, followed by assessments of the inter-observer agreement among two pathologists and two pulmonologists to evaluate endocytoscopic videos. We analyzed the nuclear features of hematoxylin-eosin-stained and endocytoscopic images from 40 and 33 cases, respectively. Endocytoscopic and hematoxylin-eosin-stained images displayed a similar tendency for each feature, despite there being no correlation. Conversely, the dimensionality reduction analyses demonstrated similar distributions of normal lung and malignant clusters in both images, thus differentiating between the clusters. The diagnostic accuracy of the pathologists was 58.3% and 52.8% (κ-value 0.38, fair), and that of the pulmonologists was 50% and 47.2% (κ-value 0.33, fair). The five nuclear features of pulmonary lesions were similar in the endocytoscopic and hematoxylin-eosin-stained images. |
| first_indexed | 2024-03-11T07:28:02Z |
| format | Article |
| id | doaj.art-d57d5099d2c3457cbc8de9c539c6634f |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T07:28:02Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-d57d5099d2c3457cbc8de9c539c6634f2023-11-17T07:31:07ZengMDPI AGDiagnostics2075-44182023-03-01135100310.3390/diagnostics13051003Comparison of Ultra-Magnifying Endocytoscopic and Hematoxylin-Eosin-Stained Images of Lung SpecimensMisato Kobayashi0Noriaki Kurimoto1Ryosuke Tanino2Yohei Shiratsuki3Takae Okuno4Mika Nakao5Takamasa Hotta6Yukari Tsubata7Makoto Nagasaki8Takashi Nishisaka9Takeshi Isobe10Division of Medical Oncology & Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, JapanDivision of Medical Oncology & Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, JapanDivision of Medical Oncology & Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, JapanDivision of Medical Oncology & Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, JapanDivision of Medical Oncology & Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, JapanDivision of Medical Oncology & Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, JapanDivision of Medical Oncology & Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, JapanDivision of Medical Oncology & Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, JapanDivision of Pathology, National Hospital Organization Hamada Medical Center, Hamada 697-8511, JapanDepartment of Pathology and Laboratory Medicine, Hiroshima Prefectural Hospital, Hiroshima 734-8530, JapanDivision of Medical Oncology & Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, JapanEndocytoscopy enables real-time observation of lesions at ultra-magnification. In the gastrointestinal and respiratory fields, endocytoscopic images are similar to hematoxylin-eosin-stained images. This study aimed to compare the nuclear features of pulmonary lesions in endocytoscopic and hematoxylin-eosin-stained images. We performed an endocytoscopy to observe resected specimens of normal lung tissue and lesions. Nuclear features were extracted using ImageJ. We analyzed five nuclear features: nuclear number per area, mean nucleus area, median circularity, coefficient of variation of roundness, and median Voronoi area. We conducted dimensionality reduction analyses for these features, followed by assessments of the inter-observer agreement among two pathologists and two pulmonologists to evaluate endocytoscopic videos. We analyzed the nuclear features of hematoxylin-eosin-stained and endocytoscopic images from 40 and 33 cases, respectively. Endocytoscopic and hematoxylin-eosin-stained images displayed a similar tendency for each feature, despite there being no correlation. Conversely, the dimensionality reduction analyses demonstrated similar distributions of normal lung and malignant clusters in both images, thus differentiating between the clusters. The diagnostic accuracy of the pathologists was 58.3% and 52.8% (κ-value 0.38, fair), and that of the pulmonologists was 50% and 47.2% (κ-value 0.33, fair). The five nuclear features of pulmonary lesions were similar in the endocytoscopic and hematoxylin-eosin-stained images.https://www.mdpi.com/2075-4418/13/5/1003endocytoscopynuclear featureperipheral pulmonary lesionlung cancer |
| spellingShingle | Misato Kobayashi Noriaki Kurimoto Ryosuke Tanino Yohei Shiratsuki Takae Okuno Mika Nakao Takamasa Hotta Yukari Tsubata Makoto Nagasaki Takashi Nishisaka Takeshi Isobe Comparison of Ultra-Magnifying Endocytoscopic and Hematoxylin-Eosin-Stained Images of Lung Specimens Diagnostics endocytoscopy nuclear feature peripheral pulmonary lesion lung cancer |
| title | Comparison of Ultra-Magnifying Endocytoscopic and Hematoxylin-Eosin-Stained Images of Lung Specimens |
| title_full | Comparison of Ultra-Magnifying Endocytoscopic and Hematoxylin-Eosin-Stained Images of Lung Specimens |
| title_fullStr | Comparison of Ultra-Magnifying Endocytoscopic and Hematoxylin-Eosin-Stained Images of Lung Specimens |
| title_full_unstemmed | Comparison of Ultra-Magnifying Endocytoscopic and Hematoxylin-Eosin-Stained Images of Lung Specimens |
| title_short | Comparison of Ultra-Magnifying Endocytoscopic and Hematoxylin-Eosin-Stained Images of Lung Specimens |
| title_sort | comparison of ultra magnifying endocytoscopic and hematoxylin eosin stained images of lung specimens |
| topic | endocytoscopy nuclear feature peripheral pulmonary lesion lung cancer |
| url | https://www.mdpi.com/2075-4418/13/5/1003 |
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