The Protective Role of <i>Scorias spongiosa</i> Polysaccharide-Based Microcapsules on Intestinal Barrier Integrity in DSS-Induced Colitis in Mice
<i>Scorias spongiosa</i>, a type of edible fungus, is beneficial for intestinal health. However, the mechanisms by which polysaccharides derived from <i>S</i>. <i>spongiosa</i> contribute to the integrity of the intestinal barrier have been little investigated. In...
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MDPI AG
2023-02-01
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| Series: | Foods |
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| author | Yingyin Xu Huiyu Feng Zhiyuan Zhang Qian Zhang Jie Tang Jie Zhou Yong Wang Weihong Peng |
| author_facet | Yingyin Xu Huiyu Feng Zhiyuan Zhang Qian Zhang Jie Tang Jie Zhou Yong Wang Weihong Peng |
| author_sort | Yingyin Xu |
| collection | DOAJ |
| description | <i>Scorias spongiosa</i>, a type of edible fungus, is beneficial for intestinal health. However, the mechanisms by which polysaccharides derived from <i>S</i>. <i>spongiosa</i> contribute to the integrity of the intestinal barrier have been little investigated. In the present study, 40 C57BL/6J mice were assigned into five groups: (1) Normal; (2) Dextran sulfate sodium (DSS)Administration; (3) DSS + Uncapped polysaccharides; (4) DSS + Low microcapsules; (5) DSS + High microcapsules. After one week of administration of <i>S. spongiosa</i> polysaccharides, all mice, excluding the Normal group, had free access to the drinking water of 3.5% DSS for seven days. Serum and feces were then taken for analysis. Scanning electron microscopy analysis indicated the structure of the micro-capped polysaccharides with curcumin was completed with a rough surface, which differs from the uncapped polysaccharides. Noticeably, <i>S. spongiosa</i> polysaccharides enhanced intestinal barrier integrity as evidenced by increasing the protein levels of Claudin-1, ZO-1 and ZO-2. Low-capped polysaccharides mitigated the DSS-induced oxidative stress by increasing catalase (CAT) concentration and decreasing malondialdehyde (MDA) and myeloperoxidase (MPO) concentrations. Besides, DSS treatment caused a disturbance of inflammation and the contents of IL-1β, IL-6, TNF-α and CRP were downregulated and the contents of IL-4, IL-10 and IFN-γ were upregulated by <i>S. spongiosa</i> polysaccharides. Research on the potential mechanisms indicated that <i>S. spongiosa</i> polysaccharides inhibited the DSS-triggered activation of NF-κB signaling. Moreover, the JAK/STAT1 and MAPK pathways were suppressed by <i>S. spongiosa</i> polysaccharides in DSS-challenged mice, with Lcap showing the strongest efficacy. 16S rDNA amplicon sequencing revealed that the richness and diversity of the microbial community were reshaped by <i>S. spongiosa</i> polysaccharide ingestion. Therefore, our study substantiated that <i>S. spongiosa</i> polysaccharides exhibited protective effects against colitis mice by reshaping the intestinal microbiome and maintaining the balance of intestinal barrier integrity, antioxidant capacity and colonic inflammation through regulation of the NF-κB–STAT1–MAPK axis. |
| first_indexed | 2024-03-11T09:44:25Z |
| format | Article |
| id | doaj.art-d58267b46d694bd6be639bd09ac8df68 |
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| last_indexed | 2024-03-11T09:44:25Z |
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| spelling | doaj.art-d58267b46d694bd6be639bd09ac8df682023-11-16T16:42:38ZengMDPI AGFoods2304-81582023-02-0112366910.3390/foods12030669The Protective Role of <i>Scorias spongiosa</i> Polysaccharide-Based Microcapsules on Intestinal Barrier Integrity in DSS-Induced Colitis in MiceYingyin Xu0Huiyu Feng1Zhiyuan Zhang2Qian Zhang3Jie Tang4Jie Zhou5Yong Wang6Weihong Peng7Sichuan Institute of Edible Fungi, Chengdu 610066, ChinaCollege of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaSichuan Institute of Edible Fungi, Chengdu 610066, ChinaSichuan Institute of Edible Fungi, Chengdu 610066, ChinaSichuan Institute of Edible Fungi, Chengdu 610066, ChinaSichuan Institute of Edible Fungi, Chengdu 610066, ChinaSichuan Institute of Edible Fungi, Chengdu 610066, ChinaSichuan Institute of Edible Fungi, Chengdu 610066, China<i>Scorias spongiosa</i>, a type of edible fungus, is beneficial for intestinal health. However, the mechanisms by which polysaccharides derived from <i>S</i>. <i>spongiosa</i> contribute to the integrity of the intestinal barrier have been little investigated. In the present study, 40 C57BL/6J mice were assigned into five groups: (1) Normal; (2) Dextran sulfate sodium (DSS)Administration; (3) DSS + Uncapped polysaccharides; (4) DSS + Low microcapsules; (5) DSS + High microcapsules. After one week of administration of <i>S. spongiosa</i> polysaccharides, all mice, excluding the Normal group, had free access to the drinking water of 3.5% DSS for seven days. Serum and feces were then taken for analysis. Scanning electron microscopy analysis indicated the structure of the micro-capped polysaccharides with curcumin was completed with a rough surface, which differs from the uncapped polysaccharides. Noticeably, <i>S. spongiosa</i> polysaccharides enhanced intestinal barrier integrity as evidenced by increasing the protein levels of Claudin-1, ZO-1 and ZO-2. Low-capped polysaccharides mitigated the DSS-induced oxidative stress by increasing catalase (CAT) concentration and decreasing malondialdehyde (MDA) and myeloperoxidase (MPO) concentrations. Besides, DSS treatment caused a disturbance of inflammation and the contents of IL-1β, IL-6, TNF-α and CRP were downregulated and the contents of IL-4, IL-10 and IFN-γ were upregulated by <i>S. spongiosa</i> polysaccharides. Research on the potential mechanisms indicated that <i>S. spongiosa</i> polysaccharides inhibited the DSS-triggered activation of NF-κB signaling. Moreover, the JAK/STAT1 and MAPK pathways were suppressed by <i>S. spongiosa</i> polysaccharides in DSS-challenged mice, with Lcap showing the strongest efficacy. 16S rDNA amplicon sequencing revealed that the richness and diversity of the microbial community were reshaped by <i>S. spongiosa</i> polysaccharide ingestion. Therefore, our study substantiated that <i>S. spongiosa</i> polysaccharides exhibited protective effects against colitis mice by reshaping the intestinal microbiome and maintaining the balance of intestinal barrier integrity, antioxidant capacity and colonic inflammation through regulation of the NF-κB–STAT1–MAPK axis.https://www.mdpi.com/2304-8158/12/3/669<i>S. spongiosa</i> polysaccharidesmicrocapsulesulcerative colitisintestinal microbiometight junctionNF-κB–STAT1–MAPK |
| spellingShingle | Yingyin Xu Huiyu Feng Zhiyuan Zhang Qian Zhang Jie Tang Jie Zhou Yong Wang Weihong Peng The Protective Role of <i>Scorias spongiosa</i> Polysaccharide-Based Microcapsules on Intestinal Barrier Integrity in DSS-Induced Colitis in Mice Foods <i>S. spongiosa</i> polysaccharides microcapsules ulcerative colitis intestinal microbiome tight junction NF-κB–STAT1–MAPK |
| title | The Protective Role of <i>Scorias spongiosa</i> Polysaccharide-Based Microcapsules on Intestinal Barrier Integrity in DSS-Induced Colitis in Mice |
| title_full | The Protective Role of <i>Scorias spongiosa</i> Polysaccharide-Based Microcapsules on Intestinal Barrier Integrity in DSS-Induced Colitis in Mice |
| title_fullStr | The Protective Role of <i>Scorias spongiosa</i> Polysaccharide-Based Microcapsules on Intestinal Barrier Integrity in DSS-Induced Colitis in Mice |
| title_full_unstemmed | The Protective Role of <i>Scorias spongiosa</i> Polysaccharide-Based Microcapsules on Intestinal Barrier Integrity in DSS-Induced Colitis in Mice |
| title_short | The Protective Role of <i>Scorias spongiosa</i> Polysaccharide-Based Microcapsules on Intestinal Barrier Integrity in DSS-Induced Colitis in Mice |
| title_sort | protective role of i scorias spongiosa i polysaccharide based microcapsules on intestinal barrier integrity in dss induced colitis in mice |
| topic | <i>S. spongiosa</i> polysaccharides microcapsules ulcerative colitis intestinal microbiome tight junction NF-κB–STAT1–MAPK |
| url | https://www.mdpi.com/2304-8158/12/3/669 |
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