Hyperdimensional Imaging Contrast Using an Optical Fiber
Fluorescence properties of a molecule can be used to study the structural and functional nature of biological processes. Physical properties, including fluorescence lifetime, emission spectrum, emission polarization, and others, help researchers probe a molecule, produce desired effects, and infer c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/21/4/1201 |
| _version_ | 1797412400538845184 |
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| author | Jenu V. Chacko Han Nim Lee Wenxin Wu Marisa S. Otegui Kevin W. Eliceiri |
| author_facet | Jenu V. Chacko Han Nim Lee Wenxin Wu Marisa S. Otegui Kevin W. Eliceiri |
| author_sort | Jenu V. Chacko |
| collection | DOAJ |
| description | Fluorescence properties of a molecule can be used to study the structural and functional nature of biological processes. Physical properties, including fluorescence lifetime, emission spectrum, emission polarization, and others, help researchers probe a molecule, produce desired effects, and infer causes and consequences. Correlative imaging techniques such as hyperdimensional imaging microscopy (HDIM) combine the physical properties and biochemical states of a fluorophore. Here we present a fiber-based imaging system that can generate hyper-dimensional contrast by combining multiple fluorescence properties into a single fluorescence lifetime decay curve. Fluorescence lifetime imaging microscopy (FLIM) with controlled excitation polarization and temporally dispersed emission can generate a spectrally coded, polarization-filtered lifetime distribution for a pixel. This HDIM scheme generates a better contrast between different molecules than that from individual techniques. This setup uses only a single detector and is simpler to implement, modular, cost-efficient, and adaptable to any existing FLIM microscope. We present higher contrast data from <i>Arabidopsis thaliana</i> epidermal cells based on intrinsic anthocyanin emission properties under multiphoton excitation. This work lays the foundation for an alternative hyperdimensional imaging system and demonstrates that contrast-based imaging is useful to study cellular heterogeneity in biological samples. |
| first_indexed | 2024-03-09T05:02:30Z |
| format | Article |
| id | doaj.art-d582e070086940b3ad84b258880b8171 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T05:02:30Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-d582e070086940b3ad84b258880b81712023-12-03T12:59:09ZengMDPI AGSensors1424-82202021-02-01214120110.3390/s21041201Hyperdimensional Imaging Contrast Using an Optical FiberJenu V. Chacko0Han Nim Lee1Wenxin Wu2Marisa S. Otegui3Kevin W. Eliceiri4Center for Quantitative Cell Imaging, University of Wisconsin, Madison, WI 53706, USACenter for Quantitative Cell Imaging, University of Wisconsin, Madison, WI 53706, USACenter for Quantitative Cell Imaging, University of Wisconsin, Madison, WI 53706, USACenter for Quantitative Cell Imaging, University of Wisconsin, Madison, WI 53706, USACenter for Quantitative Cell Imaging, University of Wisconsin, Madison, WI 53706, USAFluorescence properties of a molecule can be used to study the structural and functional nature of biological processes. Physical properties, including fluorescence lifetime, emission spectrum, emission polarization, and others, help researchers probe a molecule, produce desired effects, and infer causes and consequences. Correlative imaging techniques such as hyperdimensional imaging microscopy (HDIM) combine the physical properties and biochemical states of a fluorophore. Here we present a fiber-based imaging system that can generate hyper-dimensional contrast by combining multiple fluorescence properties into a single fluorescence lifetime decay curve. Fluorescence lifetime imaging microscopy (FLIM) with controlled excitation polarization and temporally dispersed emission can generate a spectrally coded, polarization-filtered lifetime distribution for a pixel. This HDIM scheme generates a better contrast between different molecules than that from individual techniques. This setup uses only a single detector and is simpler to implement, modular, cost-efficient, and adaptable to any existing FLIM microscope. We present higher contrast data from <i>Arabidopsis thaliana</i> epidermal cells based on intrinsic anthocyanin emission properties under multiphoton excitation. This work lays the foundation for an alternative hyperdimensional imaging system and demonstrates that contrast-based imaging is useful to study cellular heterogeneity in biological samples.https://www.mdpi.com/1424-8220/21/4/1201hyper dimensional imagingfluorescence imaginganthocyanin imaginghyper dimensional contrast imagingfluorescence lifetimepolarization |
| spellingShingle | Jenu V. Chacko Han Nim Lee Wenxin Wu Marisa S. Otegui Kevin W. Eliceiri Hyperdimensional Imaging Contrast Using an Optical Fiber Sensors hyper dimensional imaging fluorescence imaging anthocyanin imaging hyper dimensional contrast imaging fluorescence lifetime polarization |
| title | Hyperdimensional Imaging Contrast Using an Optical Fiber |
| title_full | Hyperdimensional Imaging Contrast Using an Optical Fiber |
| title_fullStr | Hyperdimensional Imaging Contrast Using an Optical Fiber |
| title_full_unstemmed | Hyperdimensional Imaging Contrast Using an Optical Fiber |
| title_short | Hyperdimensional Imaging Contrast Using an Optical Fiber |
| title_sort | hyperdimensional imaging contrast using an optical fiber |
| topic | hyper dimensional imaging fluorescence imaging anthocyanin imaging hyper dimensional contrast imaging fluorescence lifetime polarization |
| url | https://www.mdpi.com/1424-8220/21/4/1201 |
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