Co-Treatments of Edible Curcumin from Turmeric Rhizomes and Chemotherapeutic Drugs on Cytotoxicity and FLT3 Protein Expression in Leukemic Stem Cells
This study aims to enhance efficacy and reduce toxicity of the combination treatment of a drug and curcumin (Cur) on leukemic stem cell and leukemic cell lines, including KG-1a and KG-1 (FLT3<sup>+</sup> LSCs), EoL-1 (FLT3<sup>+</sup> LCs), and U937 (FLT3<sup>−</sup&...
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MDPI AG
2021-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/19/5785 |
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| author | Fah Chueahongthong Singkome Tima Sawitree Chiampanichayakul Cory Berkland Songyot Anuchapreeda |
| author_facet | Fah Chueahongthong Singkome Tima Sawitree Chiampanichayakul Cory Berkland Songyot Anuchapreeda |
| author_sort | Fah Chueahongthong |
| collection | DOAJ |
| description | This study aims to enhance efficacy and reduce toxicity of the combination treatment of a drug and curcumin (Cur) on leukemic stem cell and leukemic cell lines, including KG-1a and KG-1 (FLT3<sup>+</sup> LSCs), EoL-1 (FLT3<sup>+</sup> LCs), and U937 (FLT3<sup>−</sup> LCs). The cytotoxicity of co-treatments of doxorubicin (Dox) or idarubicin (Ida) at concentrations of the IC<sub>10</sub>–IC<sub>80</sub> values and each concentration of Cur at the IC<sub>20</sub>, IC<sub>30</sub>, IC<sub>40</sub>, and IC<sub>50</sub> values (conditions 1, 2, 3, and 4) was determined by MTT assays. Dox–Cur increased cytotoxicity in leukemic cells. Dox–Cur co-treatment showed additive and synergistic effects in several conditions. The effect of this co-treatment on FLT3 expression in KG-1a, KG-1, and EoL-1 cells was examined by Western blotting. Dox–Cur decreased FLT3 protein levels and total cell numbers in all the cell lines in a dose-dependent manner. In summary, this study exhibits a novel report of Dox–Cur co-treatment in both enhancing cytotoxicity of Dox and inhibiting cell proliferation via FLT3 protein expression in leukemia stem cells and leukemic cells. This is the option of leukemia treatment with reducing side effects of chemotherapeutic drugs to leukemia patients. |
| first_indexed | 2024-03-10T06:55:26Z |
| format | Article |
| id | doaj.art-d58354dc858c4f44affd06ab7c903b90 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:55:26Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d58354dc858c4f44affd06ab7c903b902023-11-22T16:32:35ZengMDPI AGMolecules1420-30492021-09-012619578510.3390/molecules26195785Co-Treatments of Edible Curcumin from Turmeric Rhizomes and Chemotherapeutic Drugs on Cytotoxicity and FLT3 Protein Expression in Leukemic Stem CellsFah Chueahongthong0Singkome Tima1Sawitree Chiampanichayakul2Cory Berkland3Songyot Anuchapreeda4Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas, Lawrence, KS 66047, USADivision of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandThis study aims to enhance efficacy and reduce toxicity of the combination treatment of a drug and curcumin (Cur) on leukemic stem cell and leukemic cell lines, including KG-1a and KG-1 (FLT3<sup>+</sup> LSCs), EoL-1 (FLT3<sup>+</sup> LCs), and U937 (FLT3<sup>−</sup> LCs). The cytotoxicity of co-treatments of doxorubicin (Dox) or idarubicin (Ida) at concentrations of the IC<sub>10</sub>–IC<sub>80</sub> values and each concentration of Cur at the IC<sub>20</sub>, IC<sub>30</sub>, IC<sub>40</sub>, and IC<sub>50</sub> values (conditions 1, 2, 3, and 4) was determined by MTT assays. Dox–Cur increased cytotoxicity in leukemic cells. Dox–Cur co-treatment showed additive and synergistic effects in several conditions. The effect of this co-treatment on FLT3 expression in KG-1a, KG-1, and EoL-1 cells was examined by Western blotting. Dox–Cur decreased FLT3 protein levels and total cell numbers in all the cell lines in a dose-dependent manner. In summary, this study exhibits a novel report of Dox–Cur co-treatment in both enhancing cytotoxicity of Dox and inhibiting cell proliferation via FLT3 protein expression in leukemia stem cells and leukemic cells. This is the option of leukemia treatment with reducing side effects of chemotherapeutic drugs to leukemia patients.https://www.mdpi.com/1420-3049/26/19/5785leukemialeukemic stem cellFLT-3chemotherapeutic drugcurcuminco-treatment |
| spellingShingle | Fah Chueahongthong Singkome Tima Sawitree Chiampanichayakul Cory Berkland Songyot Anuchapreeda Co-Treatments of Edible Curcumin from Turmeric Rhizomes and Chemotherapeutic Drugs on Cytotoxicity and FLT3 Protein Expression in Leukemic Stem Cells Molecules leukemia leukemic stem cell FLT-3 chemotherapeutic drug curcumin co-treatment |
| title | Co-Treatments of Edible Curcumin from Turmeric Rhizomes and Chemotherapeutic Drugs on Cytotoxicity and FLT3 Protein Expression in Leukemic Stem Cells |
| title_full | Co-Treatments of Edible Curcumin from Turmeric Rhizomes and Chemotherapeutic Drugs on Cytotoxicity and FLT3 Protein Expression in Leukemic Stem Cells |
| title_fullStr | Co-Treatments of Edible Curcumin from Turmeric Rhizomes and Chemotherapeutic Drugs on Cytotoxicity and FLT3 Protein Expression in Leukemic Stem Cells |
| title_full_unstemmed | Co-Treatments of Edible Curcumin from Turmeric Rhizomes and Chemotherapeutic Drugs on Cytotoxicity and FLT3 Protein Expression in Leukemic Stem Cells |
| title_short | Co-Treatments of Edible Curcumin from Turmeric Rhizomes and Chemotherapeutic Drugs on Cytotoxicity and FLT3 Protein Expression in Leukemic Stem Cells |
| title_sort | co treatments of edible curcumin from turmeric rhizomes and chemotherapeutic drugs on cytotoxicity and flt3 protein expression in leukemic stem cells |
| topic | leukemia leukemic stem cell FLT-3 chemotherapeutic drug curcumin co-treatment |
| url | https://www.mdpi.com/1420-3049/26/19/5785 |
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