Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction system
Abstract The development of the cardiac conduction system (CCS) is essential for correct heart function. However, critical details on the cell types populating the CCS in the mammalian heart during the development remain to be resolved. Using single-cell RNA sequencing, we generated a large dataset...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-023-02333-6 |
| _version_ | 1797453973018378240 |
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| author | Huiying Ren Xiaolin Zhou Jun Yang Kun Kou Tangting Chen Zhaoli Pu Kejun Ye Xuehui Fan Dan Zhang Xinjiang Kang Zhongcai Fan Ming Lei Tianyi Sun Xiaoqiu Tan Xianhong Ou |
| author_facet | Huiying Ren Xiaolin Zhou Jun Yang Kun Kou Tangting Chen Zhaoli Pu Kejun Ye Xuehui Fan Dan Zhang Xinjiang Kang Zhongcai Fan Ming Lei Tianyi Sun Xiaoqiu Tan Xianhong Ou |
| author_sort | Huiying Ren |
| collection | DOAJ |
| description | Abstract The development of the cardiac conduction system (CCS) is essential for correct heart function. However, critical details on the cell types populating the CCS in the mammalian heart during the development remain to be resolved. Using single-cell RNA sequencing, we generated a large dataset of transcriptomes of ~0.5 million individual cells isolated from murine hearts at six successive developmental corresponding to the early, middle and late stages of heart development. The dataset provides a powerful library for studying the development of the heart’s CCS and other cardiac components. Our initial analysis identified distinct cell types between 20 to 26 cell types across different stages, of which ten are involved in forming the CCS. Our dataset allows researchers to reuse the datasets for data mining and a wide range of analyses. Collectively, our data add valuable transcriptomic resources for further study of cardiac development, such as gene expression, transcriptional regulation and functional gene activity in developing hearts, particularly the CCS. |
| first_indexed | 2024-03-09T15:30:36Z |
| format | Article |
| id | doaj.art-d584a83cce90429bb34a534f89367688 |
| institution | Directory Open Access Journal |
| issn | 2052-4463 |
| language | English |
| last_indexed | 2024-03-09T15:30:36Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj.art-d584a83cce90429bb34a534f893676882023-11-26T12:18:25ZengNature PortfolioScientific Data2052-44632023-09-011011910.1038/s41597-023-02333-6Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction systemHuiying Ren0Xiaolin Zhou1Jun Yang2Kun Kou3Tangting Chen4Zhaoli Pu5Kejun Ye6Xuehui Fan7Dan Zhang8Xinjiang Kang9Zhongcai Fan10Ming Lei11Tianyi Sun12Xiaoqiu Tan13Xianhong Ou14Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityDepartment of Cardiology, The Affiliated Hospital of Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityDepartment of Pharmacology, University of OxfordKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityKey Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical UniversityAbstract The development of the cardiac conduction system (CCS) is essential for correct heart function. However, critical details on the cell types populating the CCS in the mammalian heart during the development remain to be resolved. Using single-cell RNA sequencing, we generated a large dataset of transcriptomes of ~0.5 million individual cells isolated from murine hearts at six successive developmental corresponding to the early, middle and late stages of heart development. The dataset provides a powerful library for studying the development of the heart’s CCS and other cardiac components. Our initial analysis identified distinct cell types between 20 to 26 cell types across different stages, of which ten are involved in forming the CCS. Our dataset allows researchers to reuse the datasets for data mining and a wide range of analyses. Collectively, our data add valuable transcriptomic resources for further study of cardiac development, such as gene expression, transcriptional regulation and functional gene activity in developing hearts, particularly the CCS.https://doi.org/10.1038/s41597-023-02333-6 |
| spellingShingle | Huiying Ren Xiaolin Zhou Jun Yang Kun Kou Tangting Chen Zhaoli Pu Kejun Ye Xuehui Fan Dan Zhang Xinjiang Kang Zhongcai Fan Ming Lei Tianyi Sun Xiaoqiu Tan Xianhong Ou Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction system Scientific Data |
| title | Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction system |
| title_full | Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction system |
| title_fullStr | Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction system |
| title_full_unstemmed | Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction system |
| title_short | Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction system |
| title_sort | single cell rna sequencing of murine hearts for studying the development of the cardiac conduction system |
| url | https://doi.org/10.1038/s41597-023-02333-6 |
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