Gut microbiota as predictors of the occurrence of high on-treatment platelet reactivity in acute ischemic stroke patients
IntroductionIn this study, we aimed to investigate the association between gut microbiota and high on-treatment platelet reactivity (HTPR) in patients with acute ischemic stroke (AIS).MethodsWe enrolled a total of 48 AIS patients, including 19 HTPR patients and 29 non-high on-treatment platelet reac...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-01-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1257317/full |
| _version_ | 1827391264472956928 |
|---|---|
| author | Zhenzhen Lou Huiying Ouyang Guixian Chen Xiaojun Li Haoxuan Chen Yibo Zhan Lilin Peng Chenghao Du Zequan Zheng Longlong Wen Haoyou Xu Min Zhao Yuanqi Zhao |
| author_facet | Zhenzhen Lou Huiying Ouyang Guixian Chen Xiaojun Li Haoxuan Chen Yibo Zhan Lilin Peng Chenghao Du Zequan Zheng Longlong Wen Haoyou Xu Min Zhao Yuanqi Zhao |
| author_sort | Zhenzhen Lou |
| collection | DOAJ |
| description | IntroductionIn this study, we aimed to investigate the association between gut microbiota and high on-treatment platelet reactivity (HTPR) in patients with acute ischemic stroke (AIS).MethodsWe enrolled a total of 48 AIS patients, including 19 HTPR patients and 29 non-high on-treatment platelet reactivity (NHTPR) patients, along with 10 healthy controls. Clinical and laboratory data, as well as stool samples, were collected from all participants. The composition and function of gut microbiota were assessed using 16S rRNA sequencing. Differences in the gut microbiota between the two groups were analyzed, and a diagnostic model based on the gut microbiota was established using random forest model.ResultsHTPR patients exhibited a decreased microbial richness compared to NHTPR patients. Additionally, the relative abundance of unidentified_Clostridia and Ralstonia was lower in HTPR patients. Significant differences in biological functions, such as toxoplasmosis, were observed between the two groups. The combination of Ralstonia, unidentified-Clostridia, Mailhella, Anaerofustis, and Aggregatibacter showed excellent predictive ability for HTPR occurrence (AUC=0.896). When comparing AIS patients with healthy controls, alterations in the microbiota structure were observed in AIS patients, with imbalances in short-chain fatty acid-producing bacteria and pathogenic bacteria. Significant differences in biological functions, such as oxidative phosphorylation, were noted between the two groups. The combination of Alloprevotella, Terrisporobacter, Streptococcus, Proteus, and unidentified_Bacteria exhibited strong predictive power for AIS occurrence (AUC=0.994).ConclusionsThis study is the first to uncover the microbial characteristics of HTPR in AIS patients and demonstrate the predictive potential of specific bacterial combinations for HTPR occurrence. |
| first_indexed | 2024-03-08T17:08:01Z |
| format | Article |
| id | doaj.art-d5884465cde44c46963102d47fd66370 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-08T17:08:01Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d5884465cde44c46963102d47fd663702024-01-04T04:30:48ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-01-011310.3389/fcimb.2023.12573171257317Gut microbiota as predictors of the occurrence of high on-treatment platelet reactivity in acute ischemic stroke patientsZhenzhen Lou0Huiying Ouyang1Guixian Chen2Xiaojun Li3Haoxuan Chen4Yibo Zhan5Lilin Peng6Chenghao Du7Zequan Zheng8Longlong Wen9Haoyou Xu10Min Zhao11Yuanqi Zhao12The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, ChinaThe Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, ChinaThe Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, ChinaThe Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, ChinaThe Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, ChinaIntroductionIn this study, we aimed to investigate the association between gut microbiota and high on-treatment platelet reactivity (HTPR) in patients with acute ischemic stroke (AIS).MethodsWe enrolled a total of 48 AIS patients, including 19 HTPR patients and 29 non-high on-treatment platelet reactivity (NHTPR) patients, along with 10 healthy controls. Clinical and laboratory data, as well as stool samples, were collected from all participants. The composition and function of gut microbiota were assessed using 16S rRNA sequencing. Differences in the gut microbiota between the two groups were analyzed, and a diagnostic model based on the gut microbiota was established using random forest model.ResultsHTPR patients exhibited a decreased microbial richness compared to NHTPR patients. Additionally, the relative abundance of unidentified_Clostridia and Ralstonia was lower in HTPR patients. Significant differences in biological functions, such as toxoplasmosis, were observed between the two groups. The combination of Ralstonia, unidentified-Clostridia, Mailhella, Anaerofustis, and Aggregatibacter showed excellent predictive ability for HTPR occurrence (AUC=0.896). When comparing AIS patients with healthy controls, alterations in the microbiota structure were observed in AIS patients, with imbalances in short-chain fatty acid-producing bacteria and pathogenic bacteria. Significant differences in biological functions, such as oxidative phosphorylation, were noted between the two groups. The combination of Alloprevotella, Terrisporobacter, Streptococcus, Proteus, and unidentified_Bacteria exhibited strong predictive power for AIS occurrence (AUC=0.994).ConclusionsThis study is the first to uncover the microbial characteristics of HTPR in AIS patients and demonstrate the predictive potential of specific bacterial combinations for HTPR occurrence.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1257317/fullacute ischemic strokehigh on-treatment platelet reactivitygut microbiota16S rRNA sequencingrandom forest model |
| spellingShingle | Zhenzhen Lou Huiying Ouyang Guixian Chen Xiaojun Li Haoxuan Chen Yibo Zhan Lilin Peng Chenghao Du Zequan Zheng Longlong Wen Haoyou Xu Min Zhao Yuanqi Zhao Gut microbiota as predictors of the occurrence of high on-treatment platelet reactivity in acute ischemic stroke patients Frontiers in Cellular and Infection Microbiology acute ischemic stroke high on-treatment platelet reactivity gut microbiota 16S rRNA sequencing random forest model |
| title | Gut microbiota as predictors of the occurrence of high on-treatment platelet reactivity in acute ischemic stroke patients |
| title_full | Gut microbiota as predictors of the occurrence of high on-treatment platelet reactivity in acute ischemic stroke patients |
| title_fullStr | Gut microbiota as predictors of the occurrence of high on-treatment platelet reactivity in acute ischemic stroke patients |
| title_full_unstemmed | Gut microbiota as predictors of the occurrence of high on-treatment platelet reactivity in acute ischemic stroke patients |
| title_short | Gut microbiota as predictors of the occurrence of high on-treatment platelet reactivity in acute ischemic stroke patients |
| title_sort | gut microbiota as predictors of the occurrence of high on treatment platelet reactivity in acute ischemic stroke patients |
| topic | acute ischemic stroke high on-treatment platelet reactivity gut microbiota 16S rRNA sequencing random forest model |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1257317/full |
| work_keys_str_mv | AT zhenzhenlou gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT huiyingouyang gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT guixianchen gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT xiaojunli gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT haoxuanchen gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT yibozhan gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT lilinpeng gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT chenghaodu gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT zequanzheng gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT longlongwen gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT haoyouxu gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT minzhao gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients AT yuanqizhao gutmicrobiotaaspredictorsoftheoccurrenceofhighontreatmentplateletreactivityinacuteischemicstrokepatients |