Mass-Spectrometry-Based Lipidome and Proteome Profiling of <i>Hottentotta saulcyi</i> (Scorpiones: Buthidae) Venom
Scorpion venom is a complex secretory mixture of components with potential biological and physiological properties that attracted many researchers due to promising applications from clinical and pharmacological perspectives. In this study, we investigated the venom of the Iranian scorpion <i>H...
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MDPI AG
2022-05-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/6/370 |
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| author | Parviz Ghezellou Kevin Jakob Javad Atashi Alireza Ghassempour Bernhard Spengler |
| author_facet | Parviz Ghezellou Kevin Jakob Javad Atashi Alireza Ghassempour Bernhard Spengler |
| author_sort | Parviz Ghezellou |
| collection | DOAJ |
| description | Scorpion venom is a complex secretory mixture of components with potential biological and physiological properties that attracted many researchers due to promising applications from clinical and pharmacological perspectives. In this study, we investigated the venom of the Iranian scorpion <i>Hottentotta saulcyi</i> (Simon, 1880) by applying mass-spectrometry-based proteomic and lipidomic approaches to assess the diversity of components present in the venom. The data revealed that the venom’s proteome composition is largely dominated by Na<sup>+</sup>- and K<sup>+</sup>-channel-impairing toxic peptides, following the enzymatic and non-enzymatic protein families, e.g., angiotensin-converting enzyme, serine protease, metalloprotease, hyaluronidase, carboxypeptidase, and cysteine-rich secretory peptide. Furthermore, lipids comprise ~1.2% of the dry weight of the crude venom. Phospholipids, ether-phospholipids, oxidized-phospholipids, triacylglycerol, cardiolipins, very-long-chain sphingomyelins, and ceramides were the most intensely detected lipid species in the scorpion venom, may acting either independently or synergistically during the envenomation alongside proteins and peptides. The results provide detailed information on the chemical makeup of the venom, helping to improve our understanding of biological molecules present in it, leading to a better insight of the medical significance of the venom, and improving the medical care of patients suffering from scorpion accidents in the relevant regions such as Iran, Iraq, Turkey, and Afghanistan. |
| first_indexed | 2024-03-09T22:19:39Z |
| format | Article |
| id | doaj.art-d588887cf3f74482913ec96662a5838b |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T22:19:39Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-d588887cf3f74482913ec96662a5838b2023-11-23T19:16:47ZengMDPI AGToxins2072-66512022-05-0114637010.3390/toxins14060370Mass-Spectrometry-Based Lipidome and Proteome Profiling of <i>Hottentotta saulcyi</i> (Scorpiones: Buthidae) VenomParviz Ghezellou0Kevin Jakob1Javad Atashi2Alireza Ghassempour3Bernhard Spengler4Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, 35392 Giessen, GermanyInstitute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, 35392 Giessen, GermanyMedicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran 1983969411, IranMedicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran 1983969411, IranInstitute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, 35392 Giessen, GermanyScorpion venom is a complex secretory mixture of components with potential biological and physiological properties that attracted many researchers due to promising applications from clinical and pharmacological perspectives. In this study, we investigated the venom of the Iranian scorpion <i>Hottentotta saulcyi</i> (Simon, 1880) by applying mass-spectrometry-based proteomic and lipidomic approaches to assess the diversity of components present in the venom. The data revealed that the venom’s proteome composition is largely dominated by Na<sup>+</sup>- and K<sup>+</sup>-channel-impairing toxic peptides, following the enzymatic and non-enzymatic protein families, e.g., angiotensin-converting enzyme, serine protease, metalloprotease, hyaluronidase, carboxypeptidase, and cysteine-rich secretory peptide. Furthermore, lipids comprise ~1.2% of the dry weight of the crude venom. Phospholipids, ether-phospholipids, oxidized-phospholipids, triacylglycerol, cardiolipins, very-long-chain sphingomyelins, and ceramides were the most intensely detected lipid species in the scorpion venom, may acting either independently or synergistically during the envenomation alongside proteins and peptides. The results provide detailed information on the chemical makeup of the venom, helping to improve our understanding of biological molecules present in it, leading to a better insight of the medical significance of the venom, and improving the medical care of patients suffering from scorpion accidents in the relevant regions such as Iran, Iraq, Turkey, and Afghanistan.https://www.mdpi.com/2072-6651/14/6/370scorpionvenomproteomicslipidomics<i>Hottentotta saulcyi</i>Iran |
| spellingShingle | Parviz Ghezellou Kevin Jakob Javad Atashi Alireza Ghassempour Bernhard Spengler Mass-Spectrometry-Based Lipidome and Proteome Profiling of <i>Hottentotta saulcyi</i> (Scorpiones: Buthidae) Venom Toxins scorpion venom proteomics lipidomics <i>Hottentotta saulcyi</i> Iran |
| title | Mass-Spectrometry-Based Lipidome and Proteome Profiling of <i>Hottentotta saulcyi</i> (Scorpiones: Buthidae) Venom |
| title_full | Mass-Spectrometry-Based Lipidome and Proteome Profiling of <i>Hottentotta saulcyi</i> (Scorpiones: Buthidae) Venom |
| title_fullStr | Mass-Spectrometry-Based Lipidome and Proteome Profiling of <i>Hottentotta saulcyi</i> (Scorpiones: Buthidae) Venom |
| title_full_unstemmed | Mass-Spectrometry-Based Lipidome and Proteome Profiling of <i>Hottentotta saulcyi</i> (Scorpiones: Buthidae) Venom |
| title_short | Mass-Spectrometry-Based Lipidome and Proteome Profiling of <i>Hottentotta saulcyi</i> (Scorpiones: Buthidae) Venom |
| title_sort | mass spectrometry based lipidome and proteome profiling of i hottentotta saulcyi i scorpiones buthidae venom |
| topic | scorpion venom proteomics lipidomics <i>Hottentotta saulcyi</i> Iran |
| url | https://www.mdpi.com/2072-6651/14/6/370 |
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