QTL Analysis for Chlorophyll Content in Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Leaves
Chlorophyll is an important factor facilitating plants to capture, allocate and transforms light energy and plays a major role in yield formation. Strawberry is one of the most important fruit crops worldwide. Breeding strawberry for better light utilization by improving photosynthetic efficiency ca...
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MDPI AG
2021-11-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/11/11/1163 |
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| author | Muhammad Irfan Siddique Koeun Han Jieun Lee Eun Su Lee Ye-Rin Lee Hye-Eun Lee Sun Yi Lee Do-Sun Kim |
| author_facet | Muhammad Irfan Siddique Koeun Han Jieun Lee Eun Su Lee Ye-Rin Lee Hye-Eun Lee Sun Yi Lee Do-Sun Kim |
| author_sort | Muhammad Irfan Siddique |
| collection | DOAJ |
| description | Chlorophyll is an important factor facilitating plants to capture, allocate and transforms light energy and plays a major role in yield formation. Strawberry is one of the most important fruit crops worldwide. Breeding strawberry for better light utilization by improving photosynthetic efficiency can improve the yield potential. In strawberry, genetic studies have been done for several traits, but no reports on the genetic mapping of chlorophyll content in leaves. In the present study, we used two independent F<sub>2</sub> mapping populations (BS-F<sub>2</sub> and BC-F<sub>2</sub>) and, Axiom 35 K strawberry chip and genotyping-by-sequencing derived single nucleotide polymorphisms based linkage maps to identify the quantitative trait loci (QTLs) controlling leaf chlorophyll content. SPAD values were used to estimate the leaf chlorophyll content of parental lines and F<sub>2</sub> populations. A total of seven QTLs, including major and minor effects, common and specific to populations, were identified across the strawberry genome explaining phenotypic variation (R<sup>2</sup>) ranging from 1.4 to 26.4%. Candidate genes associated with the photosynthesis and chlorophyll content were inferred in commonly detected QTLs. This work thus provides not only information for novel loci controlling chlorophyll content in strawberry leaves but also forms the basis for future marker assisted breeding in strawberry to select the plants for required chlorophyll content. |
| first_indexed | 2024-03-10T05:48:29Z |
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| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T05:48:29Z |
| publishDate | 2021-11-01 |
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| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-d58cea2575664cd489a4d4c8ed828d3a2023-11-22T22:00:09ZengMDPI AGAgriculture2077-04722021-11-011111116310.3390/agriculture11111163QTL Analysis for Chlorophyll Content in Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) LeavesMuhammad Irfan Siddique0Koeun Han1Jieun Lee2Eun Su Lee3Ye-Rin Lee4Hye-Eun Lee5Sun Yi Lee6Do-Sun Kim7Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, KoreaVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, KoreaVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, KoreaVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, KoreaVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, KoreaVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, KoreaVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, KoreaVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, KoreaChlorophyll is an important factor facilitating plants to capture, allocate and transforms light energy and plays a major role in yield formation. Strawberry is one of the most important fruit crops worldwide. Breeding strawberry for better light utilization by improving photosynthetic efficiency can improve the yield potential. In strawberry, genetic studies have been done for several traits, but no reports on the genetic mapping of chlorophyll content in leaves. In the present study, we used two independent F<sub>2</sub> mapping populations (BS-F<sub>2</sub> and BC-F<sub>2</sub>) and, Axiom 35 K strawberry chip and genotyping-by-sequencing derived single nucleotide polymorphisms based linkage maps to identify the quantitative trait loci (QTLs) controlling leaf chlorophyll content. SPAD values were used to estimate the leaf chlorophyll content of parental lines and F<sub>2</sub> populations. A total of seven QTLs, including major and minor effects, common and specific to populations, were identified across the strawberry genome explaining phenotypic variation (R<sup>2</sup>) ranging from 1.4 to 26.4%. Candidate genes associated with the photosynthesis and chlorophyll content were inferred in commonly detected QTLs. This work thus provides not only information for novel loci controlling chlorophyll content in strawberry leaves but also forms the basis for future marker assisted breeding in strawberry to select the plants for required chlorophyll content.https://www.mdpi.com/2077-0472/11/11/1163<i>Fragaria</i> × <i>ananassa</i>chlorophyll contentQTL analysisleaf colorSPAD values |
| spellingShingle | Muhammad Irfan Siddique Koeun Han Jieun Lee Eun Su Lee Ye-Rin Lee Hye-Eun Lee Sun Yi Lee Do-Sun Kim QTL Analysis for Chlorophyll Content in Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Leaves Agriculture <i>Fragaria</i> × <i>ananassa</i> chlorophyll content QTL analysis leaf color SPAD values |
| title | QTL Analysis for Chlorophyll Content in Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Leaves |
| title_full | QTL Analysis for Chlorophyll Content in Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Leaves |
| title_fullStr | QTL Analysis for Chlorophyll Content in Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Leaves |
| title_full_unstemmed | QTL Analysis for Chlorophyll Content in Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Leaves |
| title_short | QTL Analysis for Chlorophyll Content in Strawberry (<i>Fragaria</i> × <i>ananassa</i> Duch.) Leaves |
| title_sort | qtl analysis for chlorophyll content in strawberry i fragaria i i ananassa i duch leaves |
| topic | <i>Fragaria</i> × <i>ananassa</i> chlorophyll content QTL analysis leaf color SPAD values |
| url | https://www.mdpi.com/2077-0472/11/11/1163 |
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