Protective effect of grape seed and skin extract on high dosage garlic-induced renal oxidative stress
In this study, the protective role of grape seed and skin extract (GSSE) against high garlic dose-induced renal toxicity has been evaluated. Rats were intraperitoneally injected with garlic (5 g/kg bw) or GSSE (500 mg/kg bw) or a combination of garlic and GSSE at the same doses daily for one month....
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2012-06-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/10482 |
| _version_ | 1819034634646716416 |
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| author | Sonia Hamlaoui Oumayma Samti Meherzia Mokni Nadia Limam Alice Carrier Ferid Limam Mohamed Amri Ezzedine Aouani Lamjed Marzouki |
| author_facet | Sonia Hamlaoui Oumayma Samti Meherzia Mokni Nadia Limam Alice Carrier Ferid Limam Mohamed Amri Ezzedine Aouani Lamjed Marzouki |
| author_sort | Sonia Hamlaoui |
| collection | DOAJ |
| description | In this study, the protective role of grape seed and skin extract (GSSE) against high garlic dose-induced renal toxicity has been evaluated. Rats were intraperitoneally injected with garlic (5 g/kg bw) or GSSE (500 mg/kg bw) or a combination of garlic and GSSE at the same doses daily for one month. Renal oxidative stress markers and antioxidant status were evaluated. We also measured plasma creatinine and urea. Data showed that high garlic dose induced renal toxicity by increasing creatinine and urea and a pro-oxidative status characterized by increased malondialdehyde, carbonyl protein, calcium and H2O2, but decreased free iron. Unexpectedly garlic increased catalase but decreased peroxidase and superoxide dismutase activities. GSSE co-treatment counteracted almost all garlic-induced deleterious effects. In conclusion, high garlic dose induced a pro-oxidative state characterized by the Fenton reaction between H2O2 and free iron, inducing Ca2+ depletion, while GSSE exerted antioxidant properties and Ca2+ repletion. |
| first_indexed | 2024-12-21T07:36:51Z |
| format | Article |
| id | doaj.art-d58edae32ab94078898f3b50cc537f3e |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-21T07:36:51Z |
| publishDate | 2012-06-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-d58edae32ab94078898f3b50cc537f3e2022-12-21T19:11:26ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882012-06-017210.3329/bjp.v7i2.10482Protective effect of grape seed and skin extract on high dosage garlic-induced renal oxidative stressSonia Hamlaoui0Oumayma Samti1Meherzia Mokni2Nadia Limam3Alice Carrier4Ferid Limam5Mohamed Amri6Ezzedine Aouani7Lamjed Marzouki8Laboratoire de neurophysiologie fonctionnelle et pathologies, Département des sciences biologiques, Faculté des sciences de Tunis, Campus Universitaire El Manar II-2092 TunisLaboratoire de neurophysiologie fonctionnelle et pathologies, Département des sciences biologiques, Faculté des sciences de Tunis, Campus Universitaire El Manar II-2092 TunisLaboratoire de neurophysiologie fonctionnelle et pathologies, Département des sciences biologiques, Faculté des sciences de Tunis, Campus Universitaire El Manar II-2092 TunisLaboratoire de neurophysiologie fonctionnelle et pathologies, Département des sciences biologiques, Faculté des sciences de Tunis, Campus Universitaire El Manar II-2092 TunisINSERM, U624 « Stress cellulaire », Case 915 Parc Scientifique de Luminy, 13288 Marseille Cedex 9Laboratoire des substances bioactives, Centre de biotechnologie, Technopole Borj-Cedria, BP-901, 2050 Hammam-Lif, TunisLaboratoire de neurophysiologie fonctionnelle et pathologies, Département des sciences biologiques, Faculté des sciences de Tunis, Campus Universitaire El Manar II-2092 TunisLaboratoire des substances bioactives, Centre de biotechnologie, Technopole Borj-Cedria, BP-901, 2050 Hammam-Lif, TunisLaboratoire de neurophysiologie fonctionnelle et pathologies, Département des sciences biologiques, Faculté des sciences de Tunis, Campus Universitaire El Manar II-2092 TunisIn this study, the protective role of grape seed and skin extract (GSSE) against high garlic dose-induced renal toxicity has been evaluated. Rats were intraperitoneally injected with garlic (5 g/kg bw) or GSSE (500 mg/kg bw) or a combination of garlic and GSSE at the same doses daily for one month. Renal oxidative stress markers and antioxidant status were evaluated. We also measured plasma creatinine and urea. Data showed that high garlic dose induced renal toxicity by increasing creatinine and urea and a pro-oxidative status characterized by increased malondialdehyde, carbonyl protein, calcium and H2O2, but decreased free iron. Unexpectedly garlic increased catalase but decreased peroxidase and superoxide dismutase activities. GSSE co-treatment counteracted almost all garlic-induced deleterious effects. In conclusion, high garlic dose induced a pro-oxidative state characterized by the Fenton reaction between H2O2 and free iron, inducing Ca2+ depletion, while GSSE exerted antioxidant properties and Ca2+ repletion.https://www.banglajol.info/index.php/BJP/article/view/10482GarlicGrape seedKidneyLipid peroxidationOxidative stress |
| spellingShingle | Sonia Hamlaoui Oumayma Samti Meherzia Mokni Nadia Limam Alice Carrier Ferid Limam Mohamed Amri Ezzedine Aouani Lamjed Marzouki Protective effect of grape seed and skin extract on high dosage garlic-induced renal oxidative stress Bangladesh Journal of Pharmacology Garlic Grape seed Kidney Lipid peroxidation Oxidative stress |
| title | Protective effect of grape seed and skin extract on high dosage garlic-induced renal oxidative stress |
| title_full | Protective effect of grape seed and skin extract on high dosage garlic-induced renal oxidative stress |
| title_fullStr | Protective effect of grape seed and skin extract on high dosage garlic-induced renal oxidative stress |
| title_full_unstemmed | Protective effect of grape seed and skin extract on high dosage garlic-induced renal oxidative stress |
| title_short | Protective effect of grape seed and skin extract on high dosage garlic-induced renal oxidative stress |
| title_sort | protective effect of grape seed and skin extract on high dosage garlic induced renal oxidative stress |
| topic | Garlic Grape seed Kidney Lipid peroxidation Oxidative stress |
| url | https://www.banglajol.info/index.php/BJP/article/view/10482 |
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