Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo Investigation
Bacterial-associated wound infections are an obstacle for individuals and the medical industry. Developing versatile, antibiotic-free therapies helps heal wounds more quickly and efficiently. In the current study, fungal metabolites were employed as a reducing agent in fabricating selenium nanoparti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/9/2341 |
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| author | Heba El-Sayed Mostafa Y. Morad Hana Sonbol Olfat A. Hammam Rehab M. Abd El-Hameed Rania A. Ellethy Amina M. Ibrahim Marwa A. Hamada |
| author_facet | Heba El-Sayed Mostafa Y. Morad Hana Sonbol Olfat A. Hammam Rehab M. Abd El-Hameed Rania A. Ellethy Amina M. Ibrahim Marwa A. Hamada |
| author_sort | Heba El-Sayed |
| collection | DOAJ |
| description | Bacterial-associated wound infections are an obstacle for individuals and the medical industry. Developing versatile, antibiotic-free therapies helps heal wounds more quickly and efficiently. In the current study, fungal metabolites were employed as a reducing agent in fabricating selenium nanoparticles (SeNPs) for improved antibacterial and wound healing properties. Utilizing UV-visible spectroscopy, dynamic light scattering (DLS), zeta potential, X-ray diffraction (XRD), and electron microscopic examination, the properties of the synthesized nanoparticles were extensively evaluated. Myco-synthesized SeNPs demonstrated strong antibacterial activity against <i>Staphylococcus aureus</i> ATCC 6538 with a minimum inhibitory concentration of 0.3125 mg/mL, reducing cell number and shape distortion in scanning electron microscope (SEM) images. SeNPs’ topical administration significantly reduced wound area and healing time, exhibiting the least bacterial load after six days compared to controls. After six and 11 days of treatment, SeNPs could decrease proinflammatory cytokines IL-6 and TNF-α production. The histopathological investigation showed a healed ulcer with moderate infiltration of inflammatory cells after exposing mice’s skin to SeNPs for six and 11 days. The docking interaction indicated that SeNPs were highly efficient against the IL-6 and TNF-α binding receptors. These findings imply that myco-fabricated SeNPs might be used as topically applied antimicrobial agents for treating skin infections and wounds. |
| first_indexed | 2024-03-10T22:26:32Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T22:26:32Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d59009359d4a4c96a33d4f4b1bafa1d52023-11-19T12:04:00ZengMDPI AGMicroorganisms2076-26072023-09-01119234110.3390/microorganisms11092341Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo InvestigationHeba El-Sayed0Mostafa Y. Morad1Hana Sonbol2Olfat A. Hammam3Rehab M. Abd El-Hameed4Rania A. Ellethy5Amina M. Ibrahim6Marwa A. Hamada7Botany and Microbiology Department, Faculty of Science, Helwan University, Helwan 11795, EgyptZoology and Entomology Department, Faculty of Science, Helwan University, Helwan 11795, EgyptDepartment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaPathology Department, Theodor Bilharz Research Institute, Giza 12411, EgyptBotany and Microbiology Department, Faculty of Science, Helwan University, Helwan 11795, EgyptChemistry Department, Faculty of Science, Helwan University, Ain Helwan, Cairo 11795, EgyptMedical Malacology Department, Theodor Bilharz Research Institute, Giza 12411, EgyptBotany and Microbiology Department, Faculty of Science, Helwan University, Helwan 11795, EgyptBacterial-associated wound infections are an obstacle for individuals and the medical industry. Developing versatile, antibiotic-free therapies helps heal wounds more quickly and efficiently. In the current study, fungal metabolites were employed as a reducing agent in fabricating selenium nanoparticles (SeNPs) for improved antibacterial and wound healing properties. Utilizing UV-visible spectroscopy, dynamic light scattering (DLS), zeta potential, X-ray diffraction (XRD), and electron microscopic examination, the properties of the synthesized nanoparticles were extensively evaluated. Myco-synthesized SeNPs demonstrated strong antibacterial activity against <i>Staphylococcus aureus</i> ATCC 6538 with a minimum inhibitory concentration of 0.3125 mg/mL, reducing cell number and shape distortion in scanning electron microscope (SEM) images. SeNPs’ topical administration significantly reduced wound area and healing time, exhibiting the least bacterial load after six days compared to controls. After six and 11 days of treatment, SeNPs could decrease proinflammatory cytokines IL-6 and TNF-α production. The histopathological investigation showed a healed ulcer with moderate infiltration of inflammatory cells after exposing mice’s skin to SeNPs for six and 11 days. The docking interaction indicated that SeNPs were highly efficient against the IL-6 and TNF-α binding receptors. These findings imply that myco-fabricated SeNPs might be used as topically applied antimicrobial agents for treating skin infections and wounds.https://www.mdpi.com/2076-2607/11/9/2341selenium nanoparticleswound healingantibacterial<i>Staphylococcus aureus</i>pro-inflammatory cytokinesdocking study |
| spellingShingle | Heba El-Sayed Mostafa Y. Morad Hana Sonbol Olfat A. Hammam Rehab M. Abd El-Hameed Rania A. Ellethy Amina M. Ibrahim Marwa A. Hamada Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo Investigation Microorganisms selenium nanoparticles wound healing antibacterial <i>Staphylococcus aureus</i> pro-inflammatory cytokines docking study |
| title | Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo Investigation |
| title_full | Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo Investigation |
| title_fullStr | Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo Investigation |
| title_full_unstemmed | Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo Investigation |
| title_short | Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo Investigation |
| title_sort | myco synthesized selenium nanoparticles as wound healing and antibacterial agent an in vitro and in vivo investigation |
| topic | selenium nanoparticles wound healing antibacterial <i>Staphylococcus aureus</i> pro-inflammatory cytokines docking study |
| url | https://www.mdpi.com/2076-2607/11/9/2341 |
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