Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice
Leucine is an essential amino acid that has been investigated by its participation in the regulation of whole-body metabolism and mitochondrial function. Here, we evaluated acute leucine effects on mitochondrial respiration of skeletal muscle from male Swiss mice in vitro. Additionally, we further i...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-11-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464619304682 |
| _version_ | 1819291974071484416 |
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| author | Henver Simionato Brunetta Gabriela Cristina de Paula Matheus Fritzen Manuela Sozo Cecchini Gustavo Jorge dos Santos Evelise Maria Nazari Alex Rafacho Andreza Fabro de Bem Everson Araújo Nunes |
| author_facet | Henver Simionato Brunetta Gabriela Cristina de Paula Matheus Fritzen Manuela Sozo Cecchini Gustavo Jorge dos Santos Evelise Maria Nazari Alex Rafacho Andreza Fabro de Bem Everson Araújo Nunes |
| author_sort | Henver Simionato Brunetta |
| collection | DOAJ |
| description | Leucine is an essential amino acid that has been investigated by its participation in the regulation of whole-body metabolism and mitochondrial function. Here, we evaluated acute leucine effects on mitochondrial respiration of skeletal muscle from male Swiss mice in vitro. Additionally, we further investigated the effects of 4-wk leucine ingestion (2.5% on drinking water) on skeletal muscle mitochondrial respiration and morphology of diet-induced obesity (DIO) mice. In vitro, acute leucine increased mitochondrial respiration, and these effects were abolished in the presence of rapamycin. In DIO mice, ingestion of leucine for 4-wk improved glucose tolerance and insulin responsivity. Leucine supplementation also prevented the reduction in mitochondrial respiration, size, and complexity in the soleus skeletal muscle. We conclude that the positive effects of leucine on whole-body metabolism in DIO mice are associated with improvements in skeletal muscle mitochondrial function and morphology. Furthermore, leucine acute effects on mitochondrial respiration are mTORC1 dependent. |
| first_indexed | 2024-12-24T03:47:09Z |
| format | Article |
| id | doaj.art-d59091a79e904346bf1b3ceb2c88a1f4 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T03:47:09Z |
| publishDate | 2019-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-d59091a79e904346bf1b3ceb2c88a1f42022-12-21T17:16:41ZengElsevierJournal of Functional Foods1756-46462019-11-0162Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss miceHenver Simionato Brunetta0Gabriela Cristina de Paula1Matheus Fritzen2Manuela Sozo Cecchini3Gustavo Jorge dos Santos4Evelise Maria Nazari5Alex Rafacho6Andreza Fabro de Bem7Everson Araújo Nunes8Multicenter Graduate Program in Physiological Sciences, Federal University of Santa Catarina, Brazil; Laboratory of Investigation in Chronic Diseases – LIDoC, BrazilGraduate Program in Biochemistry, Federal University of Santa Catarina, BrazilLaboratory of Investigation in Chronic Diseases – LIDoC, BrazilGraduate Program in Cellular Biology and Development, Federal University of Santa Catarina, BrazilMulticenter Graduate Program in Physiological Sciences, Federal University of Santa Catarina, Brazil; Laboratory of Investigation in Chronic Diseases – LIDoC, BrazilGraduate Program in Cellular Biology and Development, Federal University of Santa Catarina, BrazilMulticenter Graduate Program in Physiological Sciences, Federal University of Santa Catarina, Brazil; Laboratory of Investigation in Chronic Diseases – LIDoC, BrazilGraduate Program in Biochemistry, Federal University of Santa Catarina, Brazil; Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, BrazilMulticenter Graduate Program in Physiological Sciences, Federal University of Santa Catarina, Brazil; Laboratory of Investigation in Chronic Diseases – LIDoC, Brazil; Corresponding author at: Department of Physiological Sciences, Centre of Biological Sciences, Federal University of Santa Catarina, 88040-370 Florianopolis, Santa Catarina, Brazil.Leucine is an essential amino acid that has been investigated by its participation in the regulation of whole-body metabolism and mitochondrial function. Here, we evaluated acute leucine effects on mitochondrial respiration of skeletal muscle from male Swiss mice in vitro. Additionally, we further investigated the effects of 4-wk leucine ingestion (2.5% on drinking water) on skeletal muscle mitochondrial respiration and morphology of diet-induced obesity (DIO) mice. In vitro, acute leucine increased mitochondrial respiration, and these effects were abolished in the presence of rapamycin. In DIO mice, ingestion of leucine for 4-wk improved glucose tolerance and insulin responsivity. Leucine supplementation also prevented the reduction in mitochondrial respiration, size, and complexity in the soleus skeletal muscle. We conclude that the positive effects of leucine on whole-body metabolism in DIO mice are associated with improvements in skeletal muscle mitochondrial function and morphology. Furthermore, leucine acute effects on mitochondrial respiration are mTORC1 dependent.http://www.sciencedirect.com/science/article/pii/S1756464619304682LeucineObesityMitochondriaInsulin resistanceHigh-fat diet |
| spellingShingle | Henver Simionato Brunetta Gabriela Cristina de Paula Matheus Fritzen Manuela Sozo Cecchini Gustavo Jorge dos Santos Evelise Maria Nazari Alex Rafacho Andreza Fabro de Bem Everson Araújo Nunes Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice Journal of Functional Foods Leucine Obesity Mitochondria Insulin resistance High-fat diet |
| title | Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice |
| title_full | Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice |
| title_fullStr | Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice |
| title_full_unstemmed | Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice |
| title_short | Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice |
| title_sort | leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet induced obesity in swiss mice |
| topic | Leucine Obesity Mitochondria Insulin resistance High-fat diet |
| url | http://www.sciencedirect.com/science/article/pii/S1756464619304682 |
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