Investigating neuronal function with optically controllable proteins
In the nervous system, protein activities are highly regulated in space and time. This regulation allows for fine modulation of neuronal structure and function during development and adaptive responses. For example, neurite extension and synaptogenesis both involve localized and transient activation...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-07-01
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| Series: | Frontiers in Molecular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnmol.2015.00037/full |
| _version_ | 1818023402832658432 |
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| author | Xin X Zhou Michael ePan Michael Z Lin |
| author_facet | Xin X Zhou Michael ePan Michael Z Lin |
| author_sort | Xin X Zhou |
| collection | DOAJ |
| description | In the nervous system, protein activities are highly regulated in space and time. This regulation allows for fine modulation of neuronal structure and function during development and adaptive responses. For example, neurite extension and synaptogenesis both involve localized and transient activation of cytoskeletal and signaling proteins, allowing changes in microarchitecture to occur rapidly and in a localized manner. To investigate the role of specific protein regulation events in these processes, scientists have been developing tools for optical control of the activity of specific proteins. In this review, we focus on how some recently developed optical control modules have been used in neuroscience applications. These tools have demonstrated impressive versatility in controlling various protein families and thereby cellular functions, and possess enormous potential for future applications in nervous systems. Just as optogenetic control of neuronal firings using rhodopsins has changed how we investigate circuitry function in vivo, optical control may yet yield another revolution in how we study intracellular signaling activities in the brain. |
| first_indexed | 2024-12-10T03:43:45Z |
| format | Article |
| id | doaj.art-d592015d66a14faa9ffc6fef7d9917c4 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-10T03:43:45Z |
| publishDate | 2015-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-d592015d66a14faa9ffc6fef7d9917c42022-12-22T02:03:29ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992015-07-01810.3389/fnmol.2015.00037145842Investigating neuronal function with optically controllable proteinsXin X Zhou0Michael ePan1Michael Z Lin2Stanford UniversityStanford UniversityStanford UniversityIn the nervous system, protein activities are highly regulated in space and time. This regulation allows for fine modulation of neuronal structure and function during development and adaptive responses. For example, neurite extension and synaptogenesis both involve localized and transient activation of cytoskeletal and signaling proteins, allowing changes in microarchitecture to occur rapidly and in a localized manner. To investigate the role of specific protein regulation events in these processes, scientists have been developing tools for optical control of the activity of specific proteins. In this review, we focus on how some recently developed optical control modules have been used in neuroscience applications. These tools have demonstrated impressive versatility in controlling various protein families and thereby cellular functions, and possess enormous potential for future applications in nervous systems. Just as optogenetic control of neuronal firings using rhodopsins has changed how we investigate circuitry function in vivo, optical control may yet yield another revolution in how we study intracellular signaling activities in the brain.http://journal.frontiersin.org/Journal/10.3389/fnmol.2015.00037/fullPhytochromeRhodopsinoptogeneticsG proteinssignal transduction.fluorescent proteins |
| spellingShingle | Xin X Zhou Michael ePan Michael Z Lin Investigating neuronal function with optically controllable proteins Frontiers in Molecular Neuroscience Phytochrome Rhodopsin optogenetics G proteins signal transduction. fluorescent proteins |
| title | Investigating neuronal function with optically controllable proteins |
| title_full | Investigating neuronal function with optically controllable proteins |
| title_fullStr | Investigating neuronal function with optically controllable proteins |
| title_full_unstemmed | Investigating neuronal function with optically controllable proteins |
| title_short | Investigating neuronal function with optically controllable proteins |
| title_sort | investigating neuronal function with optically controllable proteins |
| topic | Phytochrome Rhodopsin optogenetics G proteins signal transduction. fluorescent proteins |
| url | http://journal.frontiersin.org/Journal/10.3389/fnmol.2015.00037/full |
| work_keys_str_mv | AT xinxzhou investigatingneuronalfunctionwithopticallycontrollableproteins AT michaelepan investigatingneuronalfunctionwithopticallycontrollableproteins AT michaelzlin investigatingneuronalfunctionwithopticallycontrollableproteins |