Contribution of <i>elovl5a</i> to Docosahexaenoic Acid (DHA) Synthesis at the Transcriptional Regulation Level in Common Carp, <i>Cyprinus carpio</i>
Docosahexaenoic acid (DHA) is an essential nutrient for humans and plays a critical role in human development and health. Freshwater fish, such as the common carp (<i>Cyprinus carpio</i>), have a certain degree of DHA biosynthesis ability and could be a supplemental source of human DHA n...
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MDPI AG
2024-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/4/544 |
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| author | Hanyuan Zhang Peizhen Li Youxiu Zhu Yanliang Jiang Jianxin Feng Zixia Zhao Jian Xu |
| author_facet | Hanyuan Zhang Peizhen Li Youxiu Zhu Yanliang Jiang Jianxin Feng Zixia Zhao Jian Xu |
| author_sort | Hanyuan Zhang |
| collection | DOAJ |
| description | Docosahexaenoic acid (DHA) is an essential nutrient for humans and plays a critical role in human development and health. Freshwater fish, such as the common carp (<i>Cyprinus carpio</i>), have a certain degree of DHA biosynthesis ability and could be a supplemental source of human DHA needs. The elongase of very-long-chain fatty acid 5 (Elovl5) is an important enzyme affecting polyunsaturated fatty acid (PUFA) biosynthesis. However, the function and regulatory mechanism of the <i>elovl5</i> gene related to DHA synthesis in freshwater fish is not clear yet. Previous studies have found that there are two copies of the <i>elovl5</i> gene, <i>elovl5a</i> and <i>elovl5b,</i> which have different functions. Our research group found significant DHA content differences among individuals in Yellow River carp (<i>Cyprinus carpio</i> var.), and four candidate genes were found to be related to DHA synthesis through screening. In this study, the expression level of <i>elovl5a</i> is decreased in the high-DHA group compared to the low-DHA group, which indicated the down-regulation of <i>elovl5a</i> in the DHA synthesis pathways of Yellow River carp. In addition, using a dual-luciferase reporter gene assay, we found that by targeting the 3’UTR region of <i>elovl5a</i>, miR-26a-5p could regulate DHA synthesis in common carp. After CRISPR/Cas9 disruption of <i>elovl5a</i>, the DHA content in the disrupted group was significantly higher than in the wildtype group; meanwhile, the expression level of <i>elovl5a</i> in the disrupted group was significantly reduced compared with the wildtype group. These results suggest that <i>elovl5a</i> may be down-regulating DHA synthesis in Yellow River carp. This study could provide useful information for future research on the genes and pathways that affect DHA synthesis. |
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| spelling | doaj.art-d5940b1b9a3e415b8b14d73f8a985ea42024-02-23T15:04:35ZengMDPI AGAnimals2076-26152024-02-0114454410.3390/ani14040544Contribution of <i>elovl5a</i> to Docosahexaenoic Acid (DHA) Synthesis at the Transcriptional Regulation Level in Common Carp, <i>Cyprinus carpio</i>Hanyuan Zhang0Peizhen Li1Youxiu Zhu2Yanliang Jiang3Jianxin Feng4Zixia Zhao5Jian Xu6Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, ChinaKey Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, ChinaKey Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, ChinaKey Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, ChinaHenan Academy of Fishery Sciences, Zhengzhou 450044, ChinaKey Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, ChinaFisheries Engineering Institute, Chinese Academy of Fishery Sciences, Beijing 100141, ChinaDocosahexaenoic acid (DHA) is an essential nutrient for humans and plays a critical role in human development and health. Freshwater fish, such as the common carp (<i>Cyprinus carpio</i>), have a certain degree of DHA biosynthesis ability and could be a supplemental source of human DHA needs. The elongase of very-long-chain fatty acid 5 (Elovl5) is an important enzyme affecting polyunsaturated fatty acid (PUFA) biosynthesis. However, the function and regulatory mechanism of the <i>elovl5</i> gene related to DHA synthesis in freshwater fish is not clear yet. Previous studies have found that there are two copies of the <i>elovl5</i> gene, <i>elovl5a</i> and <i>elovl5b,</i> which have different functions. Our research group found significant DHA content differences among individuals in Yellow River carp (<i>Cyprinus carpio</i> var.), and four candidate genes were found to be related to DHA synthesis through screening. In this study, the expression level of <i>elovl5a</i> is decreased in the high-DHA group compared to the low-DHA group, which indicated the down-regulation of <i>elovl5a</i> in the DHA synthesis pathways of Yellow River carp. In addition, using a dual-luciferase reporter gene assay, we found that by targeting the 3’UTR region of <i>elovl5a</i>, miR-26a-5p could regulate DHA synthesis in common carp. After CRISPR/Cas9 disruption of <i>elovl5a</i>, the DHA content in the disrupted group was significantly higher than in the wildtype group; meanwhile, the expression level of <i>elovl5a</i> in the disrupted group was significantly reduced compared with the wildtype group. These results suggest that <i>elovl5a</i> may be down-regulating DHA synthesis in Yellow River carp. This study could provide useful information for future research on the genes and pathways that affect DHA synthesis.https://www.mdpi.com/2076-2615/14/4/544DHAcommon carp<i>elovl5a</i>miR-26a-5pCRISPR/Cas9 |
| spellingShingle | Hanyuan Zhang Peizhen Li Youxiu Zhu Yanliang Jiang Jianxin Feng Zixia Zhao Jian Xu Contribution of <i>elovl5a</i> to Docosahexaenoic Acid (DHA) Synthesis at the Transcriptional Regulation Level in Common Carp, <i>Cyprinus carpio</i> Animals DHA common carp <i>elovl5a</i> miR-26a-5p CRISPR/Cas9 |
| title | Contribution of <i>elovl5a</i> to Docosahexaenoic Acid (DHA) Synthesis at the Transcriptional Regulation Level in Common Carp, <i>Cyprinus carpio</i> |
| title_full | Contribution of <i>elovl5a</i> to Docosahexaenoic Acid (DHA) Synthesis at the Transcriptional Regulation Level in Common Carp, <i>Cyprinus carpio</i> |
| title_fullStr | Contribution of <i>elovl5a</i> to Docosahexaenoic Acid (DHA) Synthesis at the Transcriptional Regulation Level in Common Carp, <i>Cyprinus carpio</i> |
| title_full_unstemmed | Contribution of <i>elovl5a</i> to Docosahexaenoic Acid (DHA) Synthesis at the Transcriptional Regulation Level in Common Carp, <i>Cyprinus carpio</i> |
| title_short | Contribution of <i>elovl5a</i> to Docosahexaenoic Acid (DHA) Synthesis at the Transcriptional Regulation Level in Common Carp, <i>Cyprinus carpio</i> |
| title_sort | contribution of i elovl5a i to docosahexaenoic acid dha synthesis at the transcriptional regulation level in common carp i cyprinus carpio i |
| topic | DHA common carp <i>elovl5a</i> miR-26a-5p CRISPR/Cas9 |
| url | https://www.mdpi.com/2076-2615/14/4/544 |
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