Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure
The hepatitis C virus (HCV) encodes approximately 10 different structural and non-structural proteins, including the envelope glycoprotein 2 (E2). HCV proteins, especially the envelope proteins, bind to cell receptors and can damage tissues. Endothelial inflammation is the most important determinant...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2014-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600748&lng=en&tlng=en |
| _version_ | 1797718111647956992 |
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| author | Ana Carolina Urbaczek Lívia Carolina de Abreu Ribeiro Valdecir Farias Ximenes Ana Afonso Camila Tita Nogueira Wesley Cardoso Generoso Juliana Vieira Alberice Martina Rudnicki Renila Ferrer Luiz Marcos da Fonseca Paulo Inácio da Costa |
| author_facet | Ana Carolina Urbaczek Lívia Carolina de Abreu Ribeiro Valdecir Farias Ximenes Ana Afonso Camila Tita Nogueira Wesley Cardoso Generoso Juliana Vieira Alberice Martina Rudnicki Renila Ferrer Luiz Marcos da Fonseca Paulo Inácio da Costa |
| author_sort | Ana Carolina Urbaczek |
| collection | DOAJ |
| description | The hepatitis C virus (HCV) encodes approximately 10 different structural and non-structural proteins, including the envelope glycoprotein 2 (E2). HCV proteins, especially the envelope proteins, bind to cell receptors and can damage tissues. Endothelial inflammation is the most important determinant of fibrosis progression and, consequently, cirrhosis. The aim of this study was to evaluate and compare the inflammatory response of endothelial cells to two recombinant forms of the HCV E2 protein produced in different expression systems (Escherichia coli and Pichia pastoris). We observed the induction of cell death and the production of nitric oxide, hydrogen peroxide, interleukin-8 and vascular endothelial growth factor A in human umbilical vein endothelial cells (HUVECs) stimulated by the two recombinant E2 proteins. The E2-induced apoptosis of HUVECs was confirmed using the molecular marker PARP. The apoptosis rescue observed when the antioxidant N-acetylcysteine was used suggests that reactive oxygen species are involved in E2-induced apoptosis. We propose that these proteins are involved in the chronic inflammation caused by HCV. |
| first_indexed | 2024-03-12T08:46:14Z |
| format | Article |
| id | doaj.art-d59507b40df14732b1ee010e9c9b5e37 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:46:14Z |
| publishDate | 2014-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-d59507b40df14732b1ee010e9c9b5e372023-09-02T16:28:30ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602014-09-01109674875610.1590/0074-0276140090S0074-02762014000600748Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposureAna Carolina UrbaczekLívia Carolina de Abreu RibeiroValdecir Farias XimenesAna AfonsoCamila Tita NogueiraWesley Cardoso GenerosoJuliana Vieira AlbericeMartina RudnickiRenila FerrerLuiz Marcos da FonsecaPaulo Inácio da CostaThe hepatitis C virus (HCV) encodes approximately 10 different structural and non-structural proteins, including the envelope glycoprotein 2 (E2). HCV proteins, especially the envelope proteins, bind to cell receptors and can damage tissues. Endothelial inflammation is the most important determinant of fibrosis progression and, consequently, cirrhosis. The aim of this study was to evaluate and compare the inflammatory response of endothelial cells to two recombinant forms of the HCV E2 protein produced in different expression systems (Escherichia coli and Pichia pastoris). We observed the induction of cell death and the production of nitric oxide, hydrogen peroxide, interleukin-8 and vascular endothelial growth factor A in human umbilical vein endothelial cells (HUVECs) stimulated by the two recombinant E2 proteins. The E2-induced apoptosis of HUVECs was confirmed using the molecular marker PARP. The apoptosis rescue observed when the antioxidant N-acetylcysteine was used suggests that reactive oxygen species are involved in E2-induced apoptosis. We propose that these proteins are involved in the chronic inflammation caused by HCV.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600748&lng=en&tlng=enHCV -E2 protein -inflammation -HUVEC |
| spellingShingle | Ana Carolina Urbaczek Lívia Carolina de Abreu Ribeiro Valdecir Farias Ximenes Ana Afonso Camila Tita Nogueira Wesley Cardoso Generoso Juliana Vieira Alberice Martina Rudnicki Renila Ferrer Luiz Marcos da Fonseca Paulo Inácio da Costa Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure Memorias do Instituto Oswaldo Cruz HCV - E2 protein - inflammation - HUVEC |
| title | Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure |
| title_full | Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure |
| title_fullStr | Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure |
| title_full_unstemmed | Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure |
| title_short | Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure |
| title_sort | inflammatory response of endothelial cells to hepatitis c virus recombinant envelope glycoprotein 2 protein exposure |
| topic | HCV - E2 protein - inflammation - HUVEC |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600748&lng=en&tlng=en |
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