Identification of Rapeseed (Brassica napus) Cultivars With a High Tolerance to Boron-Deficient Conditions
Boron (B) is an essential micronutrient for seed plants. Information on B-efficiency mechanisms and B-efficient crop and model plant genotypes is very scarce. Studies evaluating the basis and consequences of B-deficiency and B-efficiency are limited by the facts that B occurs as a trace contaminant...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-08-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01142/full |
| _version_ | 1828402928377921536 |
|---|---|
| author | Benjamin Pommerrenig Astrid Junker Isidro Abreu Annett Bieber Jacqueline Fuge Evelin Willner Manuela D. Bienert Thomas Altmann Gerd P. Bienert |
| author_facet | Benjamin Pommerrenig Astrid Junker Isidro Abreu Annett Bieber Jacqueline Fuge Evelin Willner Manuela D. Bienert Thomas Altmann Gerd P. Bienert |
| author_sort | Benjamin Pommerrenig |
| collection | DOAJ |
| description | Boron (B) is an essential micronutrient for seed plants. Information on B-efficiency mechanisms and B-efficient crop and model plant genotypes is very scarce. Studies evaluating the basis and consequences of B-deficiency and B-efficiency are limited by the facts that B occurs as a trace contaminant essentially everywhere, its bioavailability is difficult to control and soil-based B-deficiency growth systems allowing a high-throughput screening of plant populations have hitherto been lacking. The crop plant Brassica napus shows a very high sensitivity toward B-deficient conditions. To reduce B-deficiency-caused yield losses in a sustainable manner, the identification of B-efficient B. napus genotypes is indispensable. We developed a soil substrate-based cultivation system which is suitable to study plant growth in automated high-throughput phenotyping facilities under defined and repeatable soil B conditions. In a comprehensive screening, using this system with soil B concentrations below 0.1 mg B (kg soil)-1, we identified three highly B-deficiency tolerant B. napus cultivars (CR2267, CR2280, and CR2285) among a genetically diverse collection comprising 590 accessions from all over the world. The B-efficiency classification of cultivars was based on a detailed assessment of various physical and high-throughput imaging-based shoot and root growth parameters in soil substrate or in in vitro conditions, respectively. We identified cultivar-specific patterns of B-deficiency-responsive growth dynamics. Elemental analysis revealed striking differences only in B contents between contrasting genotypes when grown under B-deficient but not under standard conditions. Results indicate that B-deficiency tolerant cultivars can grow with a very limited amount of B which is clearly below previously described critical B-tissue concentration values. These results suggest a higher B utilization efficiency of CR2267, CR2280, and CR2285 which would represent a unique trait among so far identified B-efficient B. napus cultivars which are characterized by a higher B-uptake capacity. Testing various other nutrient deficiency treatments, we demonstrated that the tolerance is specific for B-deficient conditions and is not conferred by a general growth vigor at the seedling stage. The identified B-deficiency tolerant cultivars will serve as genetic and physiological “tools” to further understand the mechanisms regulating the B nutritional status in rapeseed and to develop B-efficient elite genotypes. |
| first_indexed | 2024-12-10T10:09:42Z |
| format | Article |
| id | doaj.art-d5986b677e4344cbb72a1720a4c29b7d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T10:09:42Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d5986b677e4344cbb72a1720a4c29b7d2022-12-22T01:53:10ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-08-01910.3389/fpls.2018.01142400023Identification of Rapeseed (Brassica napus) Cultivars With a High Tolerance to Boron-Deficient ConditionsBenjamin Pommerrenig0Astrid Junker1Isidro Abreu2Annett Bieber3Jacqueline Fuge4Evelin Willner5Manuela D. Bienert6Thomas Altmann7Gerd P. Bienert8Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, GermanyDepartment of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, GermanyDepartment of Biology, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, SpainDepartment of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, GermanyDepartment of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, GermanyGenebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, GermanyDepartment of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, GermanyDepartment of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, GermanyDepartment of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, GermanyBoron (B) is an essential micronutrient for seed plants. Information on B-efficiency mechanisms and B-efficient crop and model plant genotypes is very scarce. Studies evaluating the basis and consequences of B-deficiency and B-efficiency are limited by the facts that B occurs as a trace contaminant essentially everywhere, its bioavailability is difficult to control and soil-based B-deficiency growth systems allowing a high-throughput screening of plant populations have hitherto been lacking. The crop plant Brassica napus shows a very high sensitivity toward B-deficient conditions. To reduce B-deficiency-caused yield losses in a sustainable manner, the identification of B-efficient B. napus genotypes is indispensable. We developed a soil substrate-based cultivation system which is suitable to study plant growth in automated high-throughput phenotyping facilities under defined and repeatable soil B conditions. In a comprehensive screening, using this system with soil B concentrations below 0.1 mg B (kg soil)-1, we identified three highly B-deficiency tolerant B. napus cultivars (CR2267, CR2280, and CR2285) among a genetically diverse collection comprising 590 accessions from all over the world. The B-efficiency classification of cultivars was based on a detailed assessment of various physical and high-throughput imaging-based shoot and root growth parameters in soil substrate or in in vitro conditions, respectively. We identified cultivar-specific patterns of B-deficiency-responsive growth dynamics. Elemental analysis revealed striking differences only in B contents between contrasting genotypes when grown under B-deficient but not under standard conditions. Results indicate that B-deficiency tolerant cultivars can grow with a very limited amount of B which is clearly below previously described critical B-tissue concentration values. These results suggest a higher B utilization efficiency of CR2267, CR2280, and CR2285 which would represent a unique trait among so far identified B-efficient B. napus cultivars which are characterized by a higher B-uptake capacity. Testing various other nutrient deficiency treatments, we demonstrated that the tolerance is specific for B-deficient conditions and is not conferred by a general growth vigor at the seedling stage. The identified B-deficiency tolerant cultivars will serve as genetic and physiological “tools” to further understand the mechanisms regulating the B nutritional status in rapeseed and to develop B-efficient elite genotypes.https://www.frontiersin.org/article/10.3389/fpls.2018.01142/fullboronrapeseedBrassica napusphenotypingboron deficiency toleranceboron efficiency |
| spellingShingle | Benjamin Pommerrenig Astrid Junker Isidro Abreu Annett Bieber Jacqueline Fuge Evelin Willner Manuela D. Bienert Thomas Altmann Gerd P. Bienert Identification of Rapeseed (Brassica napus) Cultivars With a High Tolerance to Boron-Deficient Conditions Frontiers in Plant Science boron rapeseed Brassica napus phenotyping boron deficiency tolerance boron efficiency |
| title | Identification of Rapeseed (Brassica napus) Cultivars With a High Tolerance to Boron-Deficient Conditions |
| title_full | Identification of Rapeseed (Brassica napus) Cultivars With a High Tolerance to Boron-Deficient Conditions |
| title_fullStr | Identification of Rapeseed (Brassica napus) Cultivars With a High Tolerance to Boron-Deficient Conditions |
| title_full_unstemmed | Identification of Rapeseed (Brassica napus) Cultivars With a High Tolerance to Boron-Deficient Conditions |
| title_short | Identification of Rapeseed (Brassica napus) Cultivars With a High Tolerance to Boron-Deficient Conditions |
| title_sort | identification of rapeseed brassica napus cultivars with a high tolerance to boron deficient conditions |
| topic | boron rapeseed Brassica napus phenotyping boron deficiency tolerance boron efficiency |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01142/full |
| work_keys_str_mv | AT benjaminpommerrenig identificationofrapeseedbrassicanapuscultivarswithahightolerancetoborondeficientconditions AT astridjunker identificationofrapeseedbrassicanapuscultivarswithahightolerancetoborondeficientconditions AT isidroabreu identificationofrapeseedbrassicanapuscultivarswithahightolerancetoborondeficientconditions AT annettbieber identificationofrapeseedbrassicanapuscultivarswithahightolerancetoborondeficientconditions AT jacquelinefuge identificationofrapeseedbrassicanapuscultivarswithahightolerancetoborondeficientconditions AT evelinwillner identificationofrapeseedbrassicanapuscultivarswithahightolerancetoborondeficientconditions AT manueladbienert identificationofrapeseedbrassicanapuscultivarswithahightolerancetoborondeficientconditions AT thomasaltmann identificationofrapeseedbrassicanapuscultivarswithahightolerancetoborondeficientconditions AT gerdpbienert identificationofrapeseedbrassicanapuscultivarswithahightolerancetoborondeficientconditions |