Caenorhabditis Elegans Exhibits Morphine Addiction-like Behavior via the Opioid-like Receptor NPR-17
Addiction has become a profound societal problem worldwide, and few effective treatments are available. The nematode Caenorhabditis elegans (C. elegans) is an excellent invertebrate model to study neurobiological disease states. C. elegans reportedly developed a preference for cues that had previous...
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| Format: | Article |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.802701/full |
| _version_ | 1819173478936346624 |
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| author | Soichiro Ide Hirofumi Kunitomo Yuichi Iino Kazutaka Ikeda |
| author_facet | Soichiro Ide Hirofumi Kunitomo Yuichi Iino Kazutaka Ikeda |
| author_sort | Soichiro Ide |
| collection | DOAJ |
| description | Addiction has become a profound societal problem worldwide, and few effective treatments are available. The nematode Caenorhabditis elegans (C. elegans) is an excellent invertebrate model to study neurobiological disease states. C. elegans reportedly developed a preference for cues that had previously been paired with addictive drugs, similar to place conditioning findings in rodents. Moreover, several recent studies discovered and reported the existence of an opioid-like system in C. elegans. Still unclear, however, is whether C. elegans exhibits addictive-like behaviors for opioids, such as morphine. In the present study, we found that C. elegans exhibited dose-dependent preference for morphine using the conditioned chemosensory-cue preference (CCP) test. This preference was blocked by co-treatment with the opioid receptor antagonist naloxone. C. elegans also exhibited aversion to naloxone-precipitated withdrawal from chronic morphine exposure. The expression of morphine-induced CCP and morphine withdrawal were abolished in worms that lacked the opioid-like receptor NPR-17. Dopamine-deficient mutant (cat-2 (e1112)) worms also did not exhibit morphine-induced CCP. These results indicate that the addictive function of the opioid system exists in C. elegans, which may serve as a useful model of opioid addiction. |
| first_indexed | 2024-12-22T20:23:43Z |
| format | Article |
| id | doaj.art-d59a2f586e194e9fb3238f5ab2714d03 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-22T20:23:43Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-d59a2f586e194e9fb3238f5ab2714d032022-12-21T18:13:46ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-01-011210.3389/fphar.2021.802701802701Caenorhabditis Elegans Exhibits Morphine Addiction-like Behavior via the Opioid-like Receptor NPR-17Soichiro Ide0Hirofumi Kunitomo1Yuichi Iino2Kazutaka Ikeda3Addictive Substance Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanDepartment of Biological Sciences, Faculty of Science, Graduate School of Science, The University of Tokyo, Tokyo, JapanDepartment of Biological Sciences, Faculty of Science, Graduate School of Science, The University of Tokyo, Tokyo, JapanAddictive Substance Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanAddiction has become a profound societal problem worldwide, and few effective treatments are available. The nematode Caenorhabditis elegans (C. elegans) is an excellent invertebrate model to study neurobiological disease states. C. elegans reportedly developed a preference for cues that had previously been paired with addictive drugs, similar to place conditioning findings in rodents. Moreover, several recent studies discovered and reported the existence of an opioid-like system in C. elegans. Still unclear, however, is whether C. elegans exhibits addictive-like behaviors for opioids, such as morphine. In the present study, we found that C. elegans exhibited dose-dependent preference for morphine using the conditioned chemosensory-cue preference (CCP) test. This preference was blocked by co-treatment with the opioid receptor antagonist naloxone. C. elegans also exhibited aversion to naloxone-precipitated withdrawal from chronic morphine exposure. The expression of morphine-induced CCP and morphine withdrawal were abolished in worms that lacked the opioid-like receptor NPR-17. Dopamine-deficient mutant (cat-2 (e1112)) worms also did not exhibit morphine-induced CCP. These results indicate that the addictive function of the opioid system exists in C. elegans, which may serve as a useful model of opioid addiction.https://www.frontiersin.org/articles/10.3389/fphar.2021.802701/fullC. elegansmorphinepreferenceaddictionNPR-17 |
| spellingShingle | Soichiro Ide Hirofumi Kunitomo Yuichi Iino Kazutaka Ikeda Caenorhabditis Elegans Exhibits Morphine Addiction-like Behavior via the Opioid-like Receptor NPR-17 Frontiers in Pharmacology C. elegans morphine preference addiction NPR-17 |
| title | Caenorhabditis Elegans Exhibits Morphine Addiction-like Behavior via the Opioid-like Receptor NPR-17 |
| title_full | Caenorhabditis Elegans Exhibits Morphine Addiction-like Behavior via the Opioid-like Receptor NPR-17 |
| title_fullStr | Caenorhabditis Elegans Exhibits Morphine Addiction-like Behavior via the Opioid-like Receptor NPR-17 |
| title_full_unstemmed | Caenorhabditis Elegans Exhibits Morphine Addiction-like Behavior via the Opioid-like Receptor NPR-17 |
| title_short | Caenorhabditis Elegans Exhibits Morphine Addiction-like Behavior via the Opioid-like Receptor NPR-17 |
| title_sort | caenorhabditis elegans exhibits morphine addiction like behavior via the opioid like receptor npr 17 |
| topic | C. elegans morphine preference addiction NPR-17 |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.802701/full |
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