β-caryophyllene and docosahexaenoic acid, isolated or associated, have potential antinociceptive and anti-inflammatory effects in vitro and in vivo
Abstract Inflammation is a complex biological response involving the immune, autonomic, vascular, and somatosensory systems that occurs through the synthesis of inflammatory mediators and pain induction by the activation of nociceptors. Staphylococcus aureus, the main cause of bacteremia, is one of...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-23842-1 |
| _version_ | 1797988442934607872 |
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| author | Laís Ferraz Brito Sousa Hellen Braga Martins Oliveira Nathan das Neves Selis Lorena Lobo Brito Morbeck Talita Costa Santos Lucas Santana Coelho da Silva Jully Chayra Santos Viana Mariane Mares Reis Beatriz Almeida Sampaio Guilherme Barreto Campos Jorge Timenetsky Regiane Yatsuda Lucas Miranda Marques |
| author_facet | Laís Ferraz Brito Sousa Hellen Braga Martins Oliveira Nathan das Neves Selis Lorena Lobo Brito Morbeck Talita Costa Santos Lucas Santana Coelho da Silva Jully Chayra Santos Viana Mariane Mares Reis Beatriz Almeida Sampaio Guilherme Barreto Campos Jorge Timenetsky Regiane Yatsuda Lucas Miranda Marques |
| author_sort | Laís Ferraz Brito Sousa |
| collection | DOAJ |
| description | Abstract Inflammation is a complex biological response involving the immune, autonomic, vascular, and somatosensory systems that occurs through the synthesis of inflammatory mediators and pain induction by the activation of nociceptors. Staphylococcus aureus, the main cause of bacteremia, is one of the most common and potent causes of inflammation in public health, with worse clinical outcomes in hospitals. Antioxidant substances have been evaluated as alternative therapeutic analgesics, antioxidants, anti-inflammatory agents, antitumor agents, and bactericides. Among these, we highlight the essential oils of aromatic plants, such as β-caryophyllene (BCP), and polyunsaturated fatty acids, such as docosahexaenoic acid (DHA). The objective of this study was to evaluate the biological activities of BCP–DHA association in in vitro and in vivo experimental models of antinociception and inflammation. To determine the anti-inflammatory effects, monocytes isolated from the peripheral blood of adult male volunteers were infected with methicillin-resistant S. aureus and incubated with treatment for cytokine dosage and gene expression analysis. Antinociceptive effects were observed in the three models when comparing the control (saline) and the BCP-DHA treatment groups. For this purpose, the antinociceptive effects were evaluated in animal models using the following tests: acetic acid-induced abdominal writhing, paw edema induced by formalin intraplantar injection, and von Frey hypernociception. There was a significant reduction in the GM-CSF, TNFα, IL-1, IL-6, and IL-12 levels and an increase in IL-10 levels in the BCP-DHA treatment groups, in addition to negative regulation of the expression of the genes involved in the intracellular inflammatory signaling cascade (IL-2, IL-6, IRF7, NLRP3, and TYK2) in all groups receiving treatment, regardless of the presence of infection. Statistically significant results (p < 0.05) were obtained in the acetic acid-induced abdominal writhing test, evaluation of paw edema, evaluation of paw flinching and licking in the formalin intraplantar injection model, and the von Frey hypernociception test. Therefore, BCP and DHA, either administered individually or combined, demonstrate potent anti-inflammatory and antinociceptive effects. |
| first_indexed | 2024-04-11T08:04:10Z |
| format | Article |
| id | doaj.art-d59d8f00844b49dc94695ecec4ed44c9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T08:04:10Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d59d8f00844b49dc94695ecec4ed44c92022-12-22T04:35:37ZengNature PortfolioScientific Reports2045-23222022-11-0112111410.1038/s41598-022-23842-1β-caryophyllene and docosahexaenoic acid, isolated or associated, have potential antinociceptive and anti-inflammatory effects in vitro and in vivoLaís Ferraz Brito Sousa0Hellen Braga Martins Oliveira1Nathan das Neves Selis2Lorena Lobo Brito Morbeck3Talita Costa Santos4Lucas Santana Coelho da Silva5Jully Chayra Santos Viana6Mariane Mares Reis7Beatriz Almeida Sampaio8Guilherme Barreto Campos9Jorge Timenetsky10Regiane Yatsuda11Lucas Miranda Marques12Multidisciplinary Institute of Health, Federal University of Bahia (UFBA)University of Santa Cruz (UESC)University of Santa Cruz (UESC)Multidisciplinary Institute of Health, Federal University of Bahia (UFBA)Multidisciplinary Institute of Health, Federal University of Bahia (UFBA)University of Santa Cruz (UESC)Multidisciplinary Institute of Health, Federal University of Bahia (UFBA)Multidisciplinary Institute of Health, Federal University of Bahia (UFBA)Multidisciplinary Institute of Health, Federal University of Bahia (UFBA)Multidisciplinary Institute of Health, Federal University of Bahia (UFBA)Department of Microbiology, Institute of Biomedical Science, University of São PauloMultidisciplinary Institute of Health, Federal University of Bahia (UFBA)Multidisciplinary Institute of Health, Federal University of Bahia (UFBA)Abstract Inflammation is a complex biological response involving the immune, autonomic, vascular, and somatosensory systems that occurs through the synthesis of inflammatory mediators and pain induction by the activation of nociceptors. Staphylococcus aureus, the main cause of bacteremia, is one of the most common and potent causes of inflammation in public health, with worse clinical outcomes in hospitals. Antioxidant substances have been evaluated as alternative therapeutic analgesics, antioxidants, anti-inflammatory agents, antitumor agents, and bactericides. Among these, we highlight the essential oils of aromatic plants, such as β-caryophyllene (BCP), and polyunsaturated fatty acids, such as docosahexaenoic acid (DHA). The objective of this study was to evaluate the biological activities of BCP–DHA association in in vitro and in vivo experimental models of antinociception and inflammation. To determine the anti-inflammatory effects, monocytes isolated from the peripheral blood of adult male volunteers were infected with methicillin-resistant S. aureus and incubated with treatment for cytokine dosage and gene expression analysis. Antinociceptive effects were observed in the three models when comparing the control (saline) and the BCP-DHA treatment groups. For this purpose, the antinociceptive effects were evaluated in animal models using the following tests: acetic acid-induced abdominal writhing, paw edema induced by formalin intraplantar injection, and von Frey hypernociception. There was a significant reduction in the GM-CSF, TNFα, IL-1, IL-6, and IL-12 levels and an increase in IL-10 levels in the BCP-DHA treatment groups, in addition to negative regulation of the expression of the genes involved in the intracellular inflammatory signaling cascade (IL-2, IL-6, IRF7, NLRP3, and TYK2) in all groups receiving treatment, regardless of the presence of infection. Statistically significant results (p < 0.05) were obtained in the acetic acid-induced abdominal writhing test, evaluation of paw edema, evaluation of paw flinching and licking in the formalin intraplantar injection model, and the von Frey hypernociception test. Therefore, BCP and DHA, either administered individually or combined, demonstrate potent anti-inflammatory and antinociceptive effects.https://doi.org/10.1038/s41598-022-23842-1 |
| spellingShingle | Laís Ferraz Brito Sousa Hellen Braga Martins Oliveira Nathan das Neves Selis Lorena Lobo Brito Morbeck Talita Costa Santos Lucas Santana Coelho da Silva Jully Chayra Santos Viana Mariane Mares Reis Beatriz Almeida Sampaio Guilherme Barreto Campos Jorge Timenetsky Regiane Yatsuda Lucas Miranda Marques β-caryophyllene and docosahexaenoic acid, isolated or associated, have potential antinociceptive and anti-inflammatory effects in vitro and in vivo Scientific Reports |
| title | β-caryophyllene and docosahexaenoic acid, isolated or associated, have potential antinociceptive and anti-inflammatory effects in vitro and in vivo |
| title_full | β-caryophyllene and docosahexaenoic acid, isolated or associated, have potential antinociceptive and anti-inflammatory effects in vitro and in vivo |
| title_fullStr | β-caryophyllene and docosahexaenoic acid, isolated or associated, have potential antinociceptive and anti-inflammatory effects in vitro and in vivo |
| title_full_unstemmed | β-caryophyllene and docosahexaenoic acid, isolated or associated, have potential antinociceptive and anti-inflammatory effects in vitro and in vivo |
| title_short | β-caryophyllene and docosahexaenoic acid, isolated or associated, have potential antinociceptive and anti-inflammatory effects in vitro and in vivo |
| title_sort | β caryophyllene and docosahexaenoic acid isolated or associated have potential antinociceptive and anti inflammatory effects in vitro and in vivo |
| url | https://doi.org/10.1038/s41598-022-23842-1 |
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