Immobilization of a Bienzymatic System via Crosslinking to a Metal-Organic Framework
A leading biotechnological advancement in the field of biocatalysis is the immobilization of enzymes on solid supports to create more stable and recyclable systems. Metal-organic frameworks (MOFs) are porous materials that have been explored as solid supports for enzyme immobilization. Composed of o...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Catalysts |
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| Online Access: | https://www.mdpi.com/2073-4344/12/9/969 |
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| author | Raneem Ahmad Sydnie Rizaldo Sarah E. Shaner Daniel S. Kissel Kari L. Stone |
| author_facet | Raneem Ahmad Sydnie Rizaldo Sarah E. Shaner Daniel S. Kissel Kari L. Stone |
| author_sort | Raneem Ahmad |
| collection | DOAJ |
| description | A leading biotechnological advancement in the field of biocatalysis is the immobilization of enzymes on solid supports to create more stable and recyclable systems. Metal-organic frameworks (MOFs) are porous materials that have been explored as solid supports for enzyme immobilization. Composed of organic linkers and inorganic nodes, MOFs feature empty void space with large surface areas and have the ability to be modified post-synthesis. Our target enzyme system for immobilization is glucose oxidase (GOx) and chloroperoxidase (CPO). Glucose oxidase catalyzes the oxidation of glucose and is used for many applications in biosensing, biofuel cells, and food production. Chloroperoxidase is a fungal heme enzyme that catalyzes peroxide-dependent halogenation, oxidation, and hydroxylation. These two enzymes work sequentially in this enzyme system by GOx producing peroxide, which activates CPO that reacts with a suitable substrate. This study focuses on using a zirconium-based MOF, UiO-66-NH<sub>2</sub>, to immobilize the enzyme system via crosslinking with the MOF’s amine group on the surface of the MOF. This study investigates two different crosslinkers: disuccinimidyl glutarate (DSG) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinidimide (NHS), providing stable crosslinking of the MOF to the enzymes. The two crosslinkers are used to covalently bond CPO and GOx onto UiO-66-NH<sub>2</sub>, and a comparison of the recyclability and enzymatic activity of the single immobilization of CPO and the doubly immobilized CPO and GOx is discussed through assays and characterization analyses. The DSG-crosslinked composites displayed enhanced activity relative to the free enzyme, and all crosslinked enzyme/MOF composites demonstrated recyclability, with at least 30% of the activity being retained after four catalytic cycles. The results of this report will aid researchers in utilizing CPO as a biocatalyst that is more active and has greater recyclability. |
| first_indexed | 2024-03-10T00:29:01Z |
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| id | doaj.art-d5a092c6cafa4323b97349a0731be117 |
| institution | Directory Open Access Journal |
| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-10T00:29:01Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-d5a092c6cafa4323b97349a0731be1172023-11-23T15:30:08ZengMDPI AGCatalysts2073-43442022-08-0112996910.3390/catal12090969Immobilization of a Bienzymatic System via Crosslinking to a Metal-Organic FrameworkRaneem Ahmad0Sydnie Rizaldo1Sarah E. Shaner2Daniel S. Kissel3Kari L. Stone4Department of Chemical Engineering, University of Michigan, 500 S. State St., Ann Arbor, MI 48109, USADepartment of Chemistry, Lewis University, One University Parkway, Romeoville, IL 60446, USADepartment of Chemistry and Physics, Southeast Missouri State University, One University Plaza, Cape Girardeau, MO 63701, USADepartment of Chemistry, Lewis University, One University Parkway, Romeoville, IL 60446, USADepartment of Chemistry, Lewis University, One University Parkway, Romeoville, IL 60446, USAA leading biotechnological advancement in the field of biocatalysis is the immobilization of enzymes on solid supports to create more stable and recyclable systems. Metal-organic frameworks (MOFs) are porous materials that have been explored as solid supports for enzyme immobilization. Composed of organic linkers and inorganic nodes, MOFs feature empty void space with large surface areas and have the ability to be modified post-synthesis. Our target enzyme system for immobilization is glucose oxidase (GOx) and chloroperoxidase (CPO). Glucose oxidase catalyzes the oxidation of glucose and is used for many applications in biosensing, biofuel cells, and food production. Chloroperoxidase is a fungal heme enzyme that catalyzes peroxide-dependent halogenation, oxidation, and hydroxylation. These two enzymes work sequentially in this enzyme system by GOx producing peroxide, which activates CPO that reacts with a suitable substrate. This study focuses on using a zirconium-based MOF, UiO-66-NH<sub>2</sub>, to immobilize the enzyme system via crosslinking with the MOF’s amine group on the surface of the MOF. This study investigates two different crosslinkers: disuccinimidyl glutarate (DSG) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinidimide (NHS), providing stable crosslinking of the MOF to the enzymes. The two crosslinkers are used to covalently bond CPO and GOx onto UiO-66-NH<sub>2</sub>, and a comparison of the recyclability and enzymatic activity of the single immobilization of CPO and the doubly immobilized CPO and GOx is discussed through assays and characterization analyses. The DSG-crosslinked composites displayed enhanced activity relative to the free enzyme, and all crosslinked enzyme/MOF composites demonstrated recyclability, with at least 30% of the activity being retained after four catalytic cycles. The results of this report will aid researchers in utilizing CPO as a biocatalyst that is more active and has greater recyclability.https://www.mdpi.com/2073-4344/12/9/969enzyme immobilizationmetal-organic frameworksbiocatalysiscrosslinking of enzymes |
| spellingShingle | Raneem Ahmad Sydnie Rizaldo Sarah E. Shaner Daniel S. Kissel Kari L. Stone Immobilization of a Bienzymatic System via Crosslinking to a Metal-Organic Framework Catalysts enzyme immobilization metal-organic frameworks biocatalysis crosslinking of enzymes |
| title | Immobilization of a Bienzymatic System via Crosslinking to a Metal-Organic Framework |
| title_full | Immobilization of a Bienzymatic System via Crosslinking to a Metal-Organic Framework |
| title_fullStr | Immobilization of a Bienzymatic System via Crosslinking to a Metal-Organic Framework |
| title_full_unstemmed | Immobilization of a Bienzymatic System via Crosslinking to a Metal-Organic Framework |
| title_short | Immobilization of a Bienzymatic System via Crosslinking to a Metal-Organic Framework |
| title_sort | immobilization of a bienzymatic system via crosslinking to a metal organic framework |
| topic | enzyme immobilization metal-organic frameworks biocatalysis crosslinking of enzymes |
| url | https://www.mdpi.com/2073-4344/12/9/969 |
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