Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase
Many marine animals, ranging from corals to fishes, synchronise reproduction to lunar cycles. In the annelid Platynereis dumerilii, this timing is orchestrated by an endogenous monthly (circalunar) clock entrained by moonlight. Whereas daily (circadian) clocks cause extensive transcriptomic and prot...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-02-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/41556 |
| _version_ | 1811199788262096896 |
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| author | Sven Schenk Stephanie C Bannister Fritz J Sedlazeck Dorothea Anrather Bui Quang Minh Andrea Bileck Markus Hartl Arndt von Haeseler Christopher Gerner Florian Raible Kristin Tessmar-Raible |
| author_facet | Sven Schenk Stephanie C Bannister Fritz J Sedlazeck Dorothea Anrather Bui Quang Minh Andrea Bileck Markus Hartl Arndt von Haeseler Christopher Gerner Florian Raible Kristin Tessmar-Raible |
| author_sort | Sven Schenk |
| collection | DOAJ |
| description | Many marine animals, ranging from corals to fishes, synchronise reproduction to lunar cycles. In the annelid Platynereis dumerilii, this timing is orchestrated by an endogenous monthly (circalunar) clock entrained by moonlight. Whereas daily (circadian) clocks cause extensive transcriptomic and proteomic changes, the quality and quantity of regulations by circalunar clocks have remained largely elusive. By establishing a combined transcriptomic and proteomic profiling approach, we provide first systematic insight into the molecular changes in Platynereis heads between circalunar phases, and across sexual differentiation and maturation. Whereas maturation elicits large transcriptomic and proteomic changes, the circalunar clock exhibits only minor transcriptomic, but strong proteomic regulation. Our study provides a versatile extraction technique and comprehensive resources. It corroborates that circadian and circalunar clock effects are likely distinct and identifies key molecular brain signatures for reproduction, sex and circalunar clock phase. Examples include prepro-whitnin/proctolin and ependymin-related proteins as circalunar clock targets. |
| first_indexed | 2024-04-12T01:54:14Z |
| format | Article |
| id | doaj.art-d5a24dc19a0a43e5b43c0a2bec5a1254 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:54:14Z |
| publishDate | 2019-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-d5a24dc19a0a43e5b43c0a2bec5a12542022-12-22T03:52:52ZengeLife Sciences Publications LtdeLife2050-084X2019-02-01810.7554/eLife.41556Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phaseSven Schenk0https://orcid.org/0000-0002-7689-5854Stephanie C Bannister1Fritz J Sedlazeck2https://orcid.org/0000-0001-6040-2691Dorothea Anrather3Bui Quang Minh4https://orcid.org/0000-0002-5535-6560Andrea Bileck5https://orcid.org/0000-0002-7053-8856Markus Hartl6https://orcid.org/0000-0002-4970-7336Arndt von Haeseler7https://orcid.org/0000-0002-3366-4458Christopher Gerner8Florian Raible9https://orcid.org/0000-0002-4515-6485Kristin Tessmar-Raible10https://orcid.org/0000-0002-8038-1741Max F Perutz Laboratories, University of Vienna, Vienna BioCenter, Vienna, Austria; Research Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Vienna, AustriaMax F Perutz Laboratories, University of Vienna, Vienna BioCenter, Vienna, Austria; Research Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Vienna, AustriaCenter of Integrative Bioinformatics Vienna, Max F Perutz Laboratories, University of Vienna, Medical University of Vienna, Vienna BioCenter, Vienna, AustriaMax F Perutz Laboratories, University of Vienna, Vienna BioCenter, Vienna, Austria; Mass Spectrometry Facility, Max F Perutz Laboratories, Vienna, AustriaCenter of Integrative Bioinformatics Vienna, Max F Perutz Laboratories, University of Vienna, Medical University of Vienna, Vienna BioCenter, Vienna, AustriaResearch Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Vienna, Austria; Department of Analytical Chemistry, University of Vienna, Vienna, AustriaMax F Perutz Laboratories, University of Vienna, Vienna BioCenter, Vienna, Austria; Mass Spectrometry Facility, Max F Perutz Laboratories, Vienna, AustriaResearch Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Vienna, Austria; Center of Integrative Bioinformatics Vienna, Max F Perutz Laboratories, University of Vienna, Medical University of Vienna, Vienna BioCenter, Vienna, Austria; Bioinformatics and Computational Biology, Faculty of Computer Science, University of Vienna, Vienna, AustriaResearch Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Vienna, Austria; Department of Analytical Chemistry, University of Vienna, Vienna, AustriaMax F Perutz Laboratories, University of Vienna, Vienna BioCenter, Vienna, Austria; Research Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Vienna, AustriaResearch Platform 'Rhythms of Life', University of Vienna, Vienna BioCenter, Vienna, AustriaMany marine animals, ranging from corals to fishes, synchronise reproduction to lunar cycles. In the annelid Platynereis dumerilii, this timing is orchestrated by an endogenous monthly (circalunar) clock entrained by moonlight. Whereas daily (circadian) clocks cause extensive transcriptomic and proteomic changes, the quality and quantity of regulations by circalunar clocks have remained largely elusive. By establishing a combined transcriptomic and proteomic profiling approach, we provide first systematic insight into the molecular changes in Platynereis heads between circalunar phases, and across sexual differentiation and maturation. Whereas maturation elicits large transcriptomic and proteomic changes, the circalunar clock exhibits only minor transcriptomic, but strong proteomic regulation. Our study provides a versatile extraction technique and comprehensive resources. It corroborates that circadian and circalunar clock effects are likely distinct and identifies key molecular brain signatures for reproduction, sex and circalunar clock phase. Examples include prepro-whitnin/proctolin and ependymin-related proteins as circalunar clock targets.https://elifesciences.org/articles/41556marine biologychronobiologydevelopmentsexual differentiationproteomicstranscriptomics |
| spellingShingle | Sven Schenk Stephanie C Bannister Fritz J Sedlazeck Dorothea Anrather Bui Quang Minh Andrea Bileck Markus Hartl Arndt von Haeseler Christopher Gerner Florian Raible Kristin Tessmar-Raible Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase eLife marine biology chronobiology development sexual differentiation proteomics transcriptomics |
| title | Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase |
| title_full | Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase |
| title_fullStr | Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase |
| title_full_unstemmed | Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase |
| title_short | Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase |
| title_sort | combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex maturation and circalunar clock phase |
| topic | marine biology chronobiology development sexual differentiation proteomics transcriptomics |
| url | https://elifesciences.org/articles/41556 |
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